BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0107.p (412 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313067|ref|YP_003777159.1| methyl-accepting chemotaxis tra... 481 e-137 gi|300312531|ref|YP_003776623.1| methyl-accepting chemotaxis tra... 247 1e-66 gi|300311479|ref|YP_003775571.1| methyl-accepting chemotaxis tra... 189 4e-49 gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sen... 135 7e-33 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 118 8e-28 >gi|300313067|ref|YP_003777159.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 532 Score = 481 bits (1238), Expect = e-137, Method: Compositional matrix adjust. Identities = 229/366 (62%), Positives = 286/366 (78%), Gaps = 12/366 (3%) Query: 1 VNNMSLKQKLWLPLVFAWIALFALSLWNIYETRNLQIADRQHALIDITEMSYSIVAGLHK 60 +NN+SLK+KLWLPLVF+W+AL LS+W+ Y+TR+LQIA+R+ L D+TEM+Y+ +AG K Sbjct: 1 MNNLSLKKKLWLPLVFSWMALLFLSVWHAYQTRDLQIAERERGLQDVTEMAYTTIAGYAK 60 Query: 61 QSTEGKLSADDARKEAIARVADQRYTGSGYITLVGADSVVTMHPMSPKLNGKNMLDFKDA 120 ++GKLS +DA+K AIAR+ DQRYTG GY+TLV A+SV+ MHP++ KL+G+NMLDFKDA Sbjct: 61 LESDGKLSREDAQKAAIARIRDQRYTGDGYVTLVDANSVIVMHPINSKLDGRNMLDFKDA 120 Query: 121 KGNELYKMIAATGSSAAGEGFLEYWWPRPNETKPSPKMGFVKRFKPWNLDFVASVYQDDI 180 KGNELYKMIAATGSS AG+G+LEYWWPRP +PSPKMGFVKRFKPWN D + SVY DDI Sbjct: 121 KGNELYKMIAATGSSGAGKGYLEYWWPRPGSDQPSPKMGFVKRFKPWNWDLICSVYSDDI 180 Query: 181 QADFYRSLIKAGVVLLILGAVMSAIATLVVRNIYRSIGGEPGEASQIARKIAEGDLTGAI 240 QADF R+LI+A VVL+ LG ++S IA +V RNI+RSIGGEPGEA+++ARKIA GDLTG I Sbjct: 181 QADFRRALIQAVVVLIGLGVLLSLIAGMVSRNIFRSIGGEPGEAAEMARKIAAGDLTGVI 240 Query: 241 AVRKGDEASLVSSIAYTRDRMVDTVNNILKATDSIKESVDEIAHGNMDLSNRTEEQASSL 300 +GDE SLV+++AYTRDR+VDTV+ I +AT+SIK SVDEIA GNMDLSNRTE+QA SL Sbjct: 241 HTTQGDEHSLVAAVAYTRDRLVDTVHGIKQATESIKTSVDEIATGNMDLSNRTEQQAGSL 300 Query: 301 EETAS------------AXAAKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVS 348 EETAS A A+ L G+ Q G ++VGQ QTM + S RK+ Sbjct: 301 EETASTMEELTATVKQNAANAQHANQLASGAAQVAIQGGEVVGQVVQTMESINASSRKIV 360 Query: 349 DMIKEI 354 D+I I Sbjct: 361 DIISVI 366 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 74/108 (68%), Positives = 87/108 (80%) Query: 305 SAXAAKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVSDMIKEIAVASHEQSEG 364 SA AAKEIK LID SV +V AG+ LV +AGQTM EVV SI+ VS ++ EIA ASHEQS G Sbjct: 408 SAAAAKEIKQLIDDSVGKVDAGTHLVQRAGQTMQEVVSSIQGVSTVVSEIATASHEQSSG 467 Query: 365 IDQVNLAITQMDEVTQQNAALVEEAAAASKSMQQQGDNLVKVVGVFKL 412 I+QV AITQMDE TQQNAALVE+AAAA++S+Q Q D LV++VGVF L Sbjct: 468 IEQVGEAITQMDETTQQNAALVEQAAAAAQSLQDQADKLVRLVGVFTL 515 >gi|300312531|ref|YP_003776623.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 609 Score = 247 bits (630), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 131/366 (35%), Positives = 210/366 (57%), Gaps = 13/366 (3%) Query: 1 VNNMSLKQKLWLPLVFAWIALFALSLWNIYETRNLQIADRQHALIDITEMSYSIVAGLHK 60 + +S +KLWLPLV + IAL A+S+++ RN+++ +R+ L +I + + S+V G Sbjct: 1 MGQLSFNKKLWLPLVLSLIALLAISIFSAVMVRNIRLEERKADLTNIGQTALSLVKGYGA 60 Query: 61 QSTEGKLSADDARKEAIARVADQRYTGSGYITLVGADSVVTMHPMSPKLNGKNMLDFKDA 120 + G LS +DA+K A + RY GY ++ + ++ MHP+ +L+GK++ +FKD Sbjct: 61 LADSGALSKEDAQKLAKQAIKSLRYGTDGYFSISTSGQIIVMHPIKTELDGKDLSNFKDP 120 Query: 121 KGNELYKMIAATGSSAAGEGFLEYWWPRPNETKPSPKMGFVKRFKPWNLDFVASVYQDDI 180 GN L+ I GS G GF+ Y WP+ +P PK FV +KPW+ + Y DDI Sbjct: 121 AGNNLFVEITKAGSKPEG-GFVHYLWPKVGAAEPVPKTSFVIAYKPWDWNLTTGAYMDDI 179 Query: 181 QADFYRSLIKAGVVLLILGAVMSAIATLVVRNIYRSIGGEPGEASQIARKIAEGDLTGAI 240 A F R+L + ++ + A + A+ ++ I R+IGG+P +A+++A +IA+GDLT I Sbjct: 180 NAAFLRTLWQMALLFAAVSAALVALVIVINHGILRTIGGDPAQAAEVANRIAQGDLTPNI 239 Query: 241 AVRKGDEASLVSSIAYTRDRMVDTVNNILKATDSIKESVDEIAHGNMDLSNRTEEQASSL 300 GD +SL+ ++ RD ++ T+ NI + +I + EIA GN+DLS RTE+QA S+ Sbjct: 240 ETAPGDSSSLLYAVRSMRDSLLSTITNIKASAGTIASASQEIASGNLDLSGRTEQQAGSI 299 Query: 301 EETASAXA------------AKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVS 348 EETASA A++ L + + + G +V Q QTM + DS RK++ Sbjct: 300 EETASAMEELTATVKQNADNARQANQLAESASEVAIQGGSVVSQVVQTMEGITDSSRKIA 359 Query: 349 DMIKEI 354 D+I I Sbjct: 360 DIIGVI 365 Score = 97.8 bits (242), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 86/202 (42%), Positives = 120/202 (59%), Gaps = 21/202 (10%) Query: 223 EASQIARKIAEGDLTGAIAVRKGDEASLVSSIAYTRDRMVDT---VNNILKATDSIKESV 279 +A+Q+A +E +A++ G S+VS + T + + D+ + +I+ D I Sbjct: 322 QANQLAESASE------VAIQGG---SVVSQVVQTMEGITDSSRKIADIIGVIDGIAFQT 372 Query: 280 DEIAHGNMDLSNRTEEQASSLEETAS---------AXAAKEIKSLIDGSVQEVHAGSDLV 330 + +A + R EQ AS A AAKEIK+LID SV +V GS LV Sbjct: 373 NILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKTLIDDSVAKVDTGSKLV 432 Query: 331 GQAGQTMSEVVDSIRKVSDMIKEIAVASHEQSEGIDQVNLAITQMDEVTQQNAALVEEAA 390 QAG TM+EVV S+R+V+D++ EI+ AS EQS GI +V AITQMDE TQQNAALVE+AA Sbjct: 433 EQAGATMTEVVASVRRVTDIVGEISSASQEQSAGIAEVGRAITQMDEGTQQNAALVEQAA 492 Query: 391 AASKSMQQQGDNLVKVVGVFKL 412 AA++S+Q Q L +VG F + Sbjct: 493 AAAQSLQDQAATLAGLVGRFHV 514 >gi|300311479|ref|YP_003775571.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 603 Score = 189 bits (479), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 123/361 (34%), Positives = 189/361 (52%), Gaps = 15/361 (4%) Query: 5 SLKQKLWLPLVFAWIALFALSLWNIYETRNLQIADRQHALIDITEMSYSIVAGLHKQSTE 64 SLK +L LV W L L+ W+ + R I +R+ L + E + +V Sbjct: 3 SLKAQLLSALVVMWAGLLLLAAWSAFSARENMIDERKDGLRRVVEAANGVVKSYVADVAA 62 Query: 65 GKLSADDARKEAIARVADQRYTGSGYITLVGADSVVTMHPMSPKLNGKNMLDFKDAKGNE 124 G++S ++A+K A+ R+A RY G Y+ + + VV M P + + GKN+ D KD++G Sbjct: 63 GRVSKEEAQKNALERIAAMRYDGDNYLFIFDSRPVVLMLPSNKAMVGKNVADRKDSEGKA 122 Query: 125 LYKMIAATGSSAAGEGFLEYW--WPRPNETKPSPKMGFVKRFKPWNLDFVASVYQDDIQA 182 Y + G GF++Y P +++ +PKM +V + W+ V V+ D+QA Sbjct: 123 YYVEMMKVGQEKQ-HGFVQYMGRLPGTDDSNRTPKMSYVVHNQDWDWFIVTGVFLSDVQA 181 Query: 183 DFYRSLIKAGVVLLILGAVMSAIATLVVRNIYRSIGGEPGEASQIARKIAEGDLTGAIAV 242 F L + +LL++G V+S + ++R I S+GGEP A IA +IA GDLT + Sbjct: 182 QFRADLFRLLGILLVVGVVVSVMMLAIIRRITGSLGGEPAYAVDIATRIANGDLTVQVQT 241 Query: 243 RKGDEASLVSSIAYTRDRMVDTVNNILKATDSIKESVDEIAHGNMDLSNRTEEQASSLEE 302 R GD SL+ ++A R+R+ + I + TD+I EIA GN+DLS+RTEEQA SL Sbjct: 242 RPGDTRSLLYAMATMRERLAGVIKGIRQGTDAIDIGAREIAAGNLDLSSRTEEQAGSLAT 301 Query: 303 TAS------------AXAAKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVSDM 350 TAS A A++ L + G ++VGQ QTM + S RK++D+ Sbjct: 302 TASNMDGLTATVKQNADNARQAGQLAHSASDIATRGGEVVGQVVQTMEGITGSSRKIADI 361 Query: 351 I 351 I Sbjct: 362 I 362 Score = 103 bits (257), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 63/108 (58%), Positives = 84/108 (77%) Query: 305 SAXAAKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVSDMIKEIAVASHEQSEG 364 SA AAKEIK+LI+ SVQ V +GSD V +AGQTM EVV ++R ++D++ EI+ AS EQ G Sbjct: 407 SAQAAKEIKALIEDSVQRVGSGSDQVARAGQTMGEVVAAVRSLTDIVGEISAASEEQRNG 466 Query: 365 IDQVNLAITQMDEVTQQNAALVEEAAAASKSMQQQGDNLVKVVGVFKL 412 I+QVNLAI +MD+VTQQNAALVE+AAAA+ S+++Q L V F++ Sbjct: 467 IEQVNLAIGEMDQVTQQNAALVEQAAAAAGSLEEQARRLKAAVSTFRV 514 >gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 135 bits (339), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 70/108 (64%), Positives = 90/108 (83%) Query: 305 SAXAAKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVSDMIKEIAVASHEQSEG 364 SA AAKEIK+LID SVQ+V GS LV +AG T+SEVV S+++V+D++ EI+ AS EQ++G Sbjct: 408 SATAAKEIKTLIDNSVQQVGLGSGLVQKAGVTISEVVSSVQRVTDVMSEISAASGEQTQG 467 Query: 365 IDQVNLAITQMDEVTQQNAALVEEAAAASKSMQQQGDNLVKVVGVFKL 412 I+QVN AITQMDEVTQQNAALVEEAA+AS+S+ Q L ++VGVF+L Sbjct: 468 IEQVNQAITQMDEVTQQNAALVEEAASASQSLSHQAQRLSELVGVFRL 515 Score = 69.3 bits (168), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 51/144 (35%), Positives = 73/144 (50%), Gaps = 13/144 (9%) Query: 224 ASQIARKIAEGDLTGAIAVRKGDE-ASLVSSIAYTRDRMVDTVNNILKATDSIKESVDEI 282 A + A IA GDLT I R DE L +++A + V+ + TD+I + +I Sbjct: 223 AGEFAHLIAAGDLTADITARSDDETGQLTAALAAMNTSLRRIVSQVRSGTDAIATASGQI 282 Query: 283 AHGNMDLSNRTEEQASSLEETASAXA------------AKEIKSLIDGSVQEVHAGSDLV 330 + GN+DLS+RTEEQASSLEETASA A++ L + G +V Sbjct: 283 SSGNLDLSSRTEEQASSLEETASAMEELTSTVRQNAENARQANQLALSASTIAEEGGKVV 342 Query: 331 GQAGQTMSEVVDSIRKVSDMIKEI 354 Q +TM + S +K+ D+I I Sbjct: 343 SQVIETMGSIDASSKKIVDIISVI 366 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 118 bits (296), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 72/108 (66%), Positives = 88/108 (81%) Query: 305 SAXAAKEIKSLIDGSVQEVHAGSDLVGQAGQTMSEVVDSIRKVSDMIKEIAVASHEQSEG 364 SA AAKEIK LID SVQ+V AGS LV +AG TM+EVV S+R+V+D++ EI+ A+ EQS G Sbjct: 407 SAGAAKEIKELIDDSVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGG 466 Query: 365 IDQVNLAITQMDEVTQQNAALVEEAAAASKSMQQQGDNLVKVVGVFKL 412 I++VNLAITQMDE TQQNAALVE+AAAA+ S++ Q NL VVG FKL Sbjct: 467 INEVNLAITQMDETTQQNAALVEQAAAAADSLKGQASNLAAVVGKFKL 514 Score = 88.6 bits (218), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 72/207 (34%), Positives = 108/207 (52%), Gaps = 24/207 (11%) Query: 168 NLDFVASVYQD-DIQADFYRSLIKAG----VVLLILGAVM--SAIATLVVRNIYRSIGGE 220 NLD + + QAD S + G +V L+ V+ S +A LV N+ R + Sbjct: 163 NLDAIVKINTSGSAQADEEASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLK-- 220 Query: 221 PGEASQIARKIAEGDLTGAIAVRKGDE-ASLVSSIAYTRDRMVDTVNNILKATDSIKESV 279 EA QIA+++A GDLT I DE +L+ ++ + + D V+ + + TD+I + Sbjct: 221 --EAVQIAQRVARGDLTLRIRPAGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTAS 278 Query: 280 DEIAHGNMDLSNRTEEQASSLEETASAXA------------AKEIKSLIDGSVQEVHAGS 327 EIA GN+DLSNRTE+QA SLEETASA A++ L + G Sbjct: 279 HEIARGNLDLSNRTEQQAGSLEETASAMEELTSTVTQNADNARQANQLAATASDIASQGG 338 Query: 328 DLVGQAGQTMSEVVDSIRKVSDMIKEI 354 ++VGQ QTM + +S R+++D+I I Sbjct: 339 EVVGQVVQTMEGITESSRRIADIISVI 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,076,008 Number of extensions: 40262 Number of successful extensions: 399 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 128 Length of query: 412 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 324 Effective length of database: 1,203,190 Effective search space: 389833560 Effective search space used: 389833560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)