BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0104.p (425 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 96 4e-21 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 92 6e-20 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 75 9e-15 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 61 2e-10 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 59 6e-10 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 96.3 bits (238), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 94/350 (26%), Positives = 159/350 (45%), Gaps = 35/350 (10%) Query: 14 LAASFSIAALSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVPNLP 73 L A+ +A A Q+ +KI + PLSG A G + ++ +A D +N AKG Sbjct: 10 LTAAIGLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELN--AKG----- 62 Query: 74 FSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATTSQV 133 F+ GG K K +LV D +P+ A +L+ Q KV AV+G S T S++ Sbjct: 63 FTIGG-----KKVKFELVGEDDASDPKQATAVATKLVDQ-KVAAVIGHLNSGTTIPASKI 116 Query: 134 AERYKVPYLNPESSSASLTQRGFKWFFRTTAHDDLFVTNFYEFFKELEARKGIKISKLGA 193 +P ++P +++ TQ+GFK FR A+D ++ A + + ++ Sbjct: 117 YSDAGIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKY-----AVQKLGTKQIAV 171 Query: 194 FNENTLWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLKAAAPQVVMQSSYL 253 ++ T +G K +K G +V T K+T + + LK P VV Sbjct: 172 IDDRTAYGQGVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGM- 230 Query: 254 GDAILS--MKTYKELGFTPDMILANDAGFIDTEFVKTLGKDA---DFIITRDVWSLDLAG 308 DA+ ++ K+LG + + + I T + L D D +I + +D AG Sbjct: 231 -DAVGGPMLRQMKQLGISAKFMGGDG---ICTGSLPGLAGDGLGDDQVICAEAGGVDEAG 286 Query: 309 KNPLIKQVNDL---FNARFKINFTGNSSRTFTGLMTLADAINRAGSTEPE 355 K K ++D + +F I+ N++ + MT+ADA+ +AGS +P+ Sbjct: 287 K----KGMDDFRAAYKKKFGIDVVYNAAYAYDATMTVADAMAKAGSADPK 332 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 92.4 bits (228), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 102/371 (27%), Positives = 164/371 (44%), Gaps = 34/371 (9%) Query: 5 VFKRVFKAGL---AASFSIAALSAQAQQ-QEIKIGVIYPLSGAAASTGAEMKDALELAAD 60 +FKRV +GL AAS ++ A AQ+ Q +KIG PL+G AS G + + L +A + Sbjct: 1 MFKRV--SGLNIIAASLALIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIE 58 Query: 61 IINNGAKGVPNLPFSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVG 120 +N P + GG K K ++ D Q +P+ G A++L Q V A+VG Sbjct: 59 RLNA-------QPITVGG-----KKIKFDVIAEDDQADPKSGVAVAQKLADQ-GVKAIVG 105 Query: 121 AYFSNVTATTSQVAERYKVPYLNPESSSASLTQRGFKWFFRTTAHDDLFVTNFYEFFKEL 180 Y S VT S+V + + +S+ +TQ+GF FR A D L Sbjct: 106 PYNSGVTIPASRVYNDAGI-VVATVASNPKITQQGFATLFRVAASDSQLGGKM-----AL 159 Query: 181 EARKGIKISKLGAFNENTLWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLK 240 A K +K ++ ++ T +G + K++K G D+V T K+T T+ + +K Sbjct: 160 YAAKELKFKRVAVIDDRTAYGQGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIK 219 Query: 241 AAAPQVVMQSSYLGDAILSMKTYKELGFTPDMILANDAGFIDTEFVKTLGKDA---DFII 297 P V Y + K+LG D+ L G E + LG DA Sbjct: 220 GKKPDAVFLGGYAPQGGPIKRQMKQLGV--DVPLMGGDGICSPEMGR-LGGDAIGESVYC 276 Query: 298 TRDVWSLDLAGKNPLIKQVNDLFNARFKINFTGNSSRTFTGLMTLADAINRAGSTEPEAI 357 T+ LD A + K +D + ++ + + G+M +A A+ +A S +P+ Sbjct: 277 TQGGTMLDKAKEG---KVFSDEYQKKYNRPAETYAVSFYDGMMLIAQAMKQANSVDPKQF 333 Query: 358 RKALSETNIPG 368 AL++ + G Sbjct: 334 GPALAKISYKG 344 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 75.1 bits (183), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 98/406 (24%), Positives = 181/406 (44%), Gaps = 49/406 (12%) Query: 11 KAGLAASFSIAALSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVP 70 +A +AA+ AL++QA IKIGV P +G +A G M+D ++LA IN Sbjct: 6 QAFVAAALMSLALTSQAAD-PIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEIN------- 57 Query: 71 NLPFSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATT 130 +AGG L G +I+L+ D + + G A+ LI +EKVVA VG + V + Sbjct: 58 ----AAGG----LLGRQIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALAS 109 Query: 131 SQVAERYKVPYLNPESSSASLTQRGFK----WFFRTTAHDDLFVTNFYEFFKELEARKGI 186 + + K+P +N ++ +T++ K + FRT+A D + + EA Sbjct: 110 QRFYQEAKIPVINNVATGTVITKQFEKEKDNYVFRTSAADKIQAKMIAD-----EAVDKQ 164 Query: 187 KISKLGAFNENTLWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLKAAAPQV 246 K +K+ ++T +G + K+ D+ V Y K +T+++ K Q Sbjct: 165 KFTKVAILADSTNYGQLGREDLEKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQA 224 Query: 247 VMQSSYLGDAILSMKTYKE--------LGFTPDMILANDAGFIDTEFVKTLGKDADFIIT 298 V+ + +G + + E +G P + + N FID GK+ + Sbjct: 225 VL-TYGIGPELAQIANGMEKLNWHVPIIGSWP-LSMGN---FIDNS-----GKNGNGARM 274 Query: 299 RDVWSLDLAGKNPLIKQVNDLFNARFKINFTG---NSSRTFTGLMTLADAINRAGSTEPE 355 + D G P K + + +K++ ++++ + ++ LA AI +AG+T+ + Sbjct: 275 PQTFIQD--GNTPKRKAFIEAYQKAYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGD 332 Query: 356 AIRKALSETNIPGNKLIMPW-KGVKFDEHGQNTLSSGILLQIINGK 400 +R AL N ++ + K D+H + ++ ++ NG+ Sbjct: 333 KVRAALENLNTKVEGVVTTYDKPFTHDDHEAIKPAMVVMGEVKNGR 378 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 84/400 (21%), Positives = 158/400 (39%), Gaps = 43/400 (10%) Query: 21 AALSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVPNLPFSAGGGL 80 A L + + + IG PLSG + G M++ ++LA N +G+ GG Sbjct: 29 APLPDRQETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANR--RGL-----RIGG-- 79 Query: 81 PNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATTSQVAERYKVP 140 K +++L+ D + +P A L+ Q++V+AVVG + S + + V R V Sbjct: 80 ---KSWQLRLIAQDDRADPATAEFVARSLV-QQQVIAVVGHWTSGTSLAAAPVYHRAGVI 135 Query: 141 YLNPESSSASLTQRGFKWFFRTTAHDDLF--VTNFYEFFKELEARKGIKISKLGAFNENT 198 + P + S LT + FRT +++ + Y K LE R + I ++ T Sbjct: 136 QVTPSAMSRRLTAMSYPRIFRTIPNNESLGRLAAGYAVDK-LETRTIVTI------DDRT 188 Query: 199 LWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLKAAAPQVVM------QSSY 252 +G + + + +RG +V K++ + + + P ++ Q+ Sbjct: 189 PFGQGLAEQFARTASERGAQVVGRYSVSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGV 248 Query: 253 LGDAILSMKTYKELGFTPDMILANDAGFIDTEFVKTLGKDADFIITRDVWSLDLAGKNPL 312 L +I +K + L +P G + F+ G DA+ + + K P Sbjct: 249 LAKSIRRLKLHARLMASP--------GTLGLPFLMRAGPDANGALVLEPGPPQ--DKMPG 298 Query: 313 IKQVNDLFNARFKINFTGNSSRTFTGLMTLADAINRAGSTEPEAIRKALSETNIPGNKLI 372 K ++ F + + + + I +AGS +PE I +A+ G Sbjct: 299 WKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIRQAGSADPERIAEAMHRLRFEGVS-- 356 Query: 373 MPWKGVKFDEHGQNTLSSGILLQIINGKYYTVWPFDMATR 412 V F+E G S + ++ + ++ V F+ R Sbjct: 357 ---GPVAFNEEGDLLQPSFTMYEVKDQRWQPVARFNGLPR 393 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 59.3 bits (142), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 87/373 (23%), Positives = 149/373 (39%), Gaps = 60/373 (16%) Query: 14 LAASFSIAALSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVPNLP 73 + A+ S AAL+A +IK+GV L+G AA G +K+ LA++ IN AKG Sbjct: 15 IGATLSQAALAA-----DIKLGVAEALTGPAAKYGVAIKNGFTLASEEIN--AKG----- 62 Query: 74 FSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATTSQV 133 + G K+ LV D QG + ++LI Q+KV+AV G SN + Sbjct: 63 --------GVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPI 114 Query: 134 AERYKVPYLNPESSSASLTQRGFKWFFRTTAHDDLFVTNFYEFFKELEARKGIKISKLGA 193 A KV +++ +T G F + D+ K GIK + Sbjct: 115 ANAAKVVVFGTSNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHF----GIKKVAV-- 168 Query: 194 FNENTLWGNETTKLETKLSKDRGYDLVK------------TIVYPAKSTQLTSEVQLLKA 241 ++GN+ ++ GYD+ K T Y +++ +KA Sbjct: 169 -----IYGNDDAFTKS------GYDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKA 217 Query: 242 AAPQVVMQSSYLGDAILSMKTYKELGFTPDMILANDAGFIDTEFVKTLGKDA-DFIITRD 300 P ++ S +A + + LG I N +++ + + KDA D + Sbjct: 218 GNPDAIVCSCLAEEAANIILQTRALGMKQPFIGGNG---LNSPKLFEIAKDAGDNTLMGS 274 Query: 301 VWSLDLAGKNPLIKQVNDLFNARFKINFTGNSSRTFTGLMTLADAINRAG-----STEPE 355 WS + + P K + A+F + +++ + + +ADA+ + + + Sbjct: 275 PWSAE--NQTPANKAFITAYKAKFGADPDQFAAQAYDAMYIVADALKNVKLSGNLAKDRD 332 Query: 356 AIRKALSETNIPG 368 A+R AL I G Sbjct: 333 ALRAALPAVKIDG 345 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,197,496 Number of extensions: 47645 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 9 Length of query: 425 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 337 Effective length of database: 1,203,190 Effective search space: 405475030 Effective search space used: 405475030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)