BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0103.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 65 2e-12 gi|300310287|ref|YP_003774379.1| branched-chain amino acid ABC t... 56 1e-09 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 65.1 bits (157), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 38/107 (35%), Positives = 63/107 (58%), Gaps = 1/107 (0%) Query: 17 YYYIALGLLLLTLLANFIIERSFLGYYFRAIKDEPDAARSLGVSLSKYKQIAFAVSSFFT 76 ++Y+ L++L++L I +S LG F+A++D P A+ +GVS+ +YK AF +S+ Sbjct: 156 FFYLVAVLMVLSMLVVHRILKSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAALA 215 Query: 77 AMGGSLYAQKELYIDPASVLGTGMSIKMALVAILGGIGTLFGPLVGA 123 + G+LYA E YI P + ++I L I+GG + G L+GA Sbjct: 216 GLSGALYAYSEEYISP-NTYNFELTIMFLLAVIMGGRKSRIGSLIGA 261 >gi|300310287|ref|YP_003774379.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 347 Score = 55.8 bits (133), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 35/110 (31%), Positives = 62/110 (56%), Gaps = 2/110 (1%) Query: 18 YYIALGLLLLTLLANFIIERSFLGYYFRAIKDEPDAARSLGVSLSKYKQIAFAVSSFFTA 77 Y AL ++ + L + + +S G++F A++D AAR +GV + + K +AFA+SSF Sbjct: 178 YLFALAIVTVLTLLCWRLVQSQTGHHFIAVRDNEQAARVIGVPVLRTKLLAFAISSFVIG 237 Query: 78 MGGSLYAQKEL-YIDPASVLGTGMSIKMALVAILGGIGTLFGPLVGAGLL 126 + G L+ L ++PA S+++ + I+GG+ T+ G GA L+ Sbjct: 238 VAGVLWGFVYLRTVEPAG-FNLDRSLQILFIVIIGGLATIRGAFFGAALI 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 335,740 Number of extensions: 11060 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 112 Number of HSP's successfully gapped: 13 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)