BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0100.p (180 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312615|ref|YP_003776707.1| hypothetical protein Hsero_3318... 158 3e-40 >gi|300312615|ref|YP_003776707.1| hypothetical protein Hsero_3318 [Herbaspirillum seropedicae SmR1] Length = 284 Score = 158 bits (399), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 77/148 (52%), Positives = 99/148 (66%), Gaps = 1/148 (0%) Query: 14 SAAGLRALCRTGAFDGPTAGYADGHVQANLMIVPREYAFDFLLFCQRNPKPCPLVEVLEP 73 +A +R R+GAF G T+G A HVQ NL I+P A DFL FC+RNP PCPL+ V EP Sbjct: 26 TALEVRLAARSGAFSGHTSGVASAHVQVNLAILPAALADDFLAFCERNPIPCPLLAVSEP 85 Query: 74 GR-FEPRSAPGADLRTDVPGYRIFENGVLQQEVADISAYWRDDLVSFLIGCSFSFESALV 132 G P D+R+DVP Y ++ +G EV+D+ WRDDLV+F +GCSFSFE AL+ Sbjct: 86 GSPALPALGLDLDVRSDVPRYHLWRHGERVAEVSDLREVWRDDLVTFALGCSFSFEHALL 145 Query: 133 EGGIPLRHIEQQRNVAMYKTSMPCTPAG 160 GIPLRH+ + RNVAMY+T++P P G Sbjct: 146 AAGIPLRHVSEGRNVAMYRTNIPTQPVG 173 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 619,998 Number of extensions: 24885 Number of successful extensions: 51 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 1 Length of query: 180 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 101 Effective length of database: 1,245,805 Effective search space: 125826305 Effective search space used: 125826305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)