BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0096.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 170 2e-43 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 169 4e-43 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 166 2e-42 gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 166 3e-42 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 158 7e-40 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 170 bits (430), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 150/398 (37%), Positives = 195/398 (48%), Gaps = 48/398 (12%) Query: 1 MKHKVLAAAAFAAVGIQVVPALAQSSVTIYGLIDTGIVVESG-----GAAGHVTKLTSGI 55 MK +LA A +G A AQSSVTIYG++DTG+V +S G G L SG+ Sbjct: 1 MKKSLLA---LAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGV 57 Query: 56 SGGSRIGFKGTEDLGGGMSALFLLETGFQNDTGALGQ---GGLLFGRQSYVGLGGGFGTV 112 GSRIGFKGTEDLGGG+SA+F LE GF NDTGAL LF R+S VGL G FG+V Sbjct: 58 IQGSRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSV 117 Query: 113 TAGRQ--YTPQYLVLSAVDPFG--TGYAGDAANLMPNTGNGASRMDNTIKYASPNFGGVT 168 GRQ + ++V FG TG AG + + T R +N+I Y + N G T Sbjct: 118 LLGRQTDFADTISSYTSVQDFGGWTGAAGHNLDRLEGT-----RTNNSISYTTANLNGFT 172 Query: 169 GELAYGFGETAGSTTAASQLGAAIGYANGPLNVRLGFH-----------------GRNND 211 G L YGFGETAG T+A G Y NGPL + + ++ G N Sbjct: 173 GNLMYGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQF 232 Query: 212 TVTTQTGSGRSTLL--GATYNFGPAKLHAAYG-VNKGLNSSPLRNTVNPYGTTPI----V 264 T T GS +L A+Y FGPA+++ + V + LN+ I + Sbjct: 233 TNGTNNGSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTL 292 Query: 265 ASTDSADIL-VGVTVPFGVQHTVLAS--YIRKN-DKTALNQDADQFALGYRYALSKRTDL 320 A+++ ADI +G +LA+ + R D + Q +LG Y LSKRTDL Sbjct: 293 AASNKADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDL 352 Query: 321 YAMYAHIKNKNGAPYTVGSSIEAGSGNSAWNMGIRHTF 358 YA A+ K + V S A +GIRH F Sbjct: 353 YAFLAYSKATDLNNPGVADSSGTDGNQGAVAVGIRHKF 390 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 169 bits (428), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 140/354 (39%), Positives = 182/354 (51%), Gaps = 38/354 (10%) Query: 23 AQSSVTIYGLIDTGI-VVESGG--AAGHVTKLTSGISGGSRIGFKGTEDLGGGMSALFLL 79 AQSSVTIYGL+DTG+ + + G AG VT++TSG + SRIGF+GTEDLGGG+ ALF L Sbjct: 21 AQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKALFQL 80 Query: 80 ETGFQNDTGAL-GQGGLLFGRQSYVGLGGGFGTVTAGRQYTPQYLVLSAVDPFGTGYAGD 138 E GF+ D G+ G LF RQS VGL GGFG V AGR Y+ Y L DP G Sbjct: 81 ENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYSANYS 140 Query: 139 AANLMPNTGNGASRM----DNTIKYASPNFGGVTGELAYGFGETAGSTTAASQLGAAIGY 194 A TGN M N +KY F GV YGFGE AG T ++ G +G+ Sbjct: 141 WATTAGATGNRKDGMPTGVSNLVKYQG-EFSGVKIGAMYGFGEVAGETRNGAKYGVGLGW 199 Query: 195 ANGPLNVRLGFHGRNNDTVTTQTGSGRSTLLGATYNFGPAKLHAAYGVNKGLNSSPLRNT 254 GP +V + + RNN T T R+T + P++ A+ N G Sbjct: 200 GTGPFSV-VATYDRNNSATATNTPYDRATSAHLAAGYQPSE---AWKFNLG--------- 246 Query: 255 VNPYGTTPIVASTDSADILV-----GVTVPFGVQHTVLASYIRKNDKTALNQDAD--QFA 307 Y +T +AD+ G F T++ + +N K N DAD ++ Sbjct: 247 ---YRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVAN-DADPIMYS 302 Query: 308 LGYRYALSKRTDLYAMYAHIKNKNGAPYTVG-SSIEAGSGNSAWNM--GIRHTF 358 L +YA+SKRTDLY A+ K KNG VG S +AG G+S + M G++H F Sbjct: 303 LRLKYAMSKRTDLYLSTAYAKAKNGQ--LVGLSRDDAGYGSSQFGMTLGMQHRF 354 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 166 bits (421), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 143/379 (37%), Positives = 190/379 (50%), Gaps = 48/379 (12%) Query: 1 MKHKVLAAAAFAAVGIQVVPALAQSSVTIYGLIDTGIVV-----ESGGAAGHVTKLTSGI 55 MK +L AA A A AQSSV+IYGLID G+ +SGG + ++ SGI Sbjct: 1 MKKTLLVAAMLAG---GATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGI 57 Query: 56 SGGSRIGFKGTEDLGGGMSALFLLETGFQNDTGALGQGGLLFGRQSYVGLGG-GFGTVTA 114 + GSRIGFKGTEDLGGGM+A F LETGF D G+LGQGGLLFGRQS+VGLG +G+++ Sbjct: 58 AQGSRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSV 117 Query: 115 GRQYT------PQYLV--LSAVDPFGTGYAGDAAN--LMPNTGNGASRMDNTIKYASPNF 164 GRQY QY + LS FG G DAA+ + N R +N+IKY S F Sbjct: 118 GRQYDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKF 177 Query: 165 GGVTGELAYGFGETAGSTTAASQLGAAIGYANGPLNVRLGFHGRNNDTVTTQTGSGRSTL 224 GG + G GETAG+ A + A Y NGP + L + + TGS + + Sbjct: 178 GGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAY-----TDIKDSTGSFSTRI 232 Query: 225 L--GATYNFGPAKLHAAYGVNKGLNSSPLRNTVNPYGTTPIVASTDSADILVGVTVPFGV 282 GA Y G +GV + ++ Y + A T D+ Sbjct: 233 YGGGARYQIGA---FTPFGVVTQVKNTNTSAKATTYELGTVYALTPLWDL---------- 279 Query: 283 QHTVLASYIRKNDKTALNQDADQFALGYRYALSKRTDLY--AMYAHIKNKNGAPYTVGSS 340 A +K ++ N A + Y+ +SKRTDLY A+Y K N P G Sbjct: 280 ---AAAYQFQKRNQGVGNAQALVATVDYK--ISKRTDLYVGAVYDRDKGYNAYP-VFGGG 333 Query: 341 IEAGSGN-SAWNMGIRHTF 358 I++ +GN +A +G+RH F Sbjct: 334 IQSATGNQTALRLGMRHRF 352 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 166 bits (420), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 131/350 (37%), Positives = 176/350 (50%), Gaps = 33/350 (9%) Query: 21 ALAQSSVTIYGLIDTGIVVESGGAAGHVTKLTSGISGGSRIGFKGTEDLGGGMSALFLLE 80 ALAQS+VT+YG+IDT + + G V L SG SR+GFKG+EDLGGG+ A F +E Sbjct: 18 ALAQSNVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGFKGSEDLGGGLRANFQME 77 Query: 81 TGFQNDTGALGQGGLLFGRQSYVGLGGGFGTVTAGRQYTPQYLVLSAVDPF-GTGYAGDA 139 GF +D+GA+ LF RQS+VGLG G + GRQ +P +L+++ +D F G Y Sbjct: 78 NGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFLMIARLDAFAGATY---- 133 Query: 140 ANLMPNTGNGASRMDNTIKYASPNFGGVTGELAYGFGETAGSTTAASQLGAAIGYANGPL 199 A+ + N R DN I Y SP G + YG GE S A Y +GPL Sbjct: 134 ASFLNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAPRNGLSTAMLAGEYESGPL 193 Query: 200 NVRLGFHGRN--NDTVTTQTGSGRSTLLGATYNFGPAKLHAAYGVNKGLNSSPLRNTVNP 257 + + +N ND +T RST G Y++G K+ Y L ++ NT Sbjct: 194 YLGVSSSAQNSANDNITI-----RSTFAGGAYDYGSGKVFLGYYRGNNLGAAASANTPGS 248 Query: 258 YGTTPIVASTDSADI-LVGVTVPFGVQHTVLASYIRKNDKTALNQDADQFALGYRYALSK 316 Y A + SA+ L G Q T+ A Y D T ++A QF+L Y LS+ Sbjct: 249 Y----YSAYSLSANYRLTG-------QATLGAGYGWAKDSTGAGRNARQFSLIGTYDLSR 297 Query: 317 RTDLYAMYAHIKNKNG--------APYTVGSSIEAGSGNSAWNMGIRHTF 358 RT LYA YAH+ N NG AP T + + G+ N +GIRH F Sbjct: 298 RTMLYATYAHLANNNGASFSLAGAAPITKNAPVSGGTVNGV-QVGIRHLF 346 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 158 bits (399), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 132/368 (35%), Positives = 187/368 (50%), Gaps = 42/368 (11%) Query: 21 ALAQSSVTIYGLIDTGI-VVESGGAAGHV-TKLTSGISGGSRIGFKGTEDLGGGMSALFL 78 A AQS+VTIYG++DT I + + A G+ ++ +G SRIGFKGTEDLGGG+ A+F Sbjct: 19 AFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLKAMFQ 78 Query: 79 LETGFQNDTGALGQGGLLFGRQSYVGLGGGFGTVTAGRQYTPQY-LVLSAVDPFGTG-YA 136 LE GF +DTGA+ QGG LFGRQS+VGL GGFG V GRQ TP + L+ DP G YA Sbjct: 79 LENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQNTPLFDLLADHFDPLTVGNYA 138 Query: 137 GDAANLMPNTGNGASRMDNTIKYASPNFGGVTGELAYGFGETAGSTTAASQLGAAIGYAN 196 ++ +P + R N ++Y +FG V ++ FGE AGS SQ +A+ Y Sbjct: 139 SNS--WLPAAAS-LVRSPNMLRY-DGDFGPVAASASWAFGEKAGSVRTGSQFSSALRYTA 194 Query: 197 GPLNVRLGFHGRNNDTVTTQTGSGRSTL--LGATYNFGPAKLHAAY-------------- 240 GPL + G+ TV++ S + T L A+Y+FG AK+ + Sbjct: 195 GPLALGGGYQ----QTVSSTNSSLKHTTWNLSASYDFGVAKVFGGWLNIKDDSGLTGSVM 250 Query: 241 ----GVNKGLNSSPLRNT----VNPYGTTPIVASTDSADILVGVTVPFGVQHTVLASYIR 292 V+ G ++ N V + S+ A + +T F + SY+ Sbjct: 251 AADGDVSNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLTGAFYYDRSKNVSYV- 309 Query: 293 KNDKTALNQDADQFAL-GY-RYALSKRTDLYAMYAHIKNKNGAPYTVGSSIEAGSGNSAW 350 T D ++ L G+ YALSKRT LY + K K+ A Y + +S + + Sbjct: 310 ---STGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYELTNSANGKNSLTTV 366 Query: 351 NMGIRHTF 358 +GIRH F Sbjct: 367 GVGIRHIF 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,197,552 Number of extensions: 55025 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 14 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)