BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0094.p (541 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 397 e-111 gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis pro... 389 e-109 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 388 e-109 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 384 e-107 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 365 e-102 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 397 bits (1019), Expect = e-111, Method: Compositional matrix adjust. Identities = 249/512 (48%), Positives = 336/512 (65%), Gaps = 10/512 (1%) Query: 3 KLSHLNIGQRLGLGFGVVILLMLGLTVIGVYRLNQINGNIRSIANDFYPRTVMANTVKGA 62 KL + I RL + F V+I +M +T +G+ L+ I+ + I D Y + +A ++ Sbjct: 2 KLKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDN 61 Query: 63 LDETARSMRNLLFMSTVDEIKGELNAITRAGAVIDETLKRFARDTPSAEGKRMLDAVNAA 122 ++ AR++RN L ++E L+ A E L + + +A G+ + ++ A Sbjct: 62 VNGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQEA 121 Query: 123 RAQYTPVLDNFLAAVKDGQVEQARDLILPEIAPYQAAYFKALDNLIAFQGRGMEQAGQEA 182 R+ Y D +K + E+A +L+ ++ P Q YF+ LD +AFQ MEQ+ E Sbjct: 122 RSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEG 181 Query: 183 ERVSGAASVLMIAMATIASLLAVLVGYSVMRSITRPLNAAINIAERVAGGDLSVRVENSA 242 + +A +M+ ++ +A L V+ + V RSITRPLN A+++A VA GDL+V++ ++ Sbjct: 182 RDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGATS 241 Query: 243 HDETGKLLTALQGMRDGLLNAVTQVRDGSDAISVAAREIAAGNANLSARTELQASTLEET 302 DETG LL AL+ M D L V++VR GSD I+ A+ EIA GN +LS+RTE QA LEET Sbjct: 242 KDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEET 301 Query: 303 ASSM----------LXNARQANQLVMNASQVATQGGDVVTQVVNTMREIKDSSRQIVEII 352 AS+M NARQAN L AS+VA QGG VV QVV TM EI D+SR+IV+II Sbjct: 302 ASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDII 361 Query: 353 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRGLAQRSASAAKEIAGLIQNSV 412 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LAQRSASAAKEI LI +SV Sbjct: 362 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALIDDSV 421 Query: 413 KKVDAGSTLVDQAGNTMQDIVRSVKHVADIMTEISAASDEQSAGIRQVNDAIEQIDDMTQ 472 +VD GS LV+QAG TM ++V SV+ V D++ EISAAS+EQS GI Q+N AI Q+D +TQ Sbjct: 422 SRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQVTQ 481 Query: 473 QNSALVEQAAAAAQSMHDQSMALGRAVSIFKI 504 QN+ALVE+AAAAAQS+ +QS L VS+FK+ Sbjct: 482 QNAALVEEAAAAAQSLQEQSTRLSATVSVFKL 513 >gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 534 Score = 389 bits (999), Expect = e-109, Method: Compositional matrix adjust. Identities = 258/538 (47%), Positives = 348/538 (64%), Gaps = 17/538 (3%) Query: 3 KLSHLNIGQRLGLGFGVVILLMLGLTVIGVYRLNQINGNIRSIANDFYPRTVMANTVKGA 62 +LS++ I RL LGFG++++LML + G+ RL Q+N ++ + +D YP+TV+A + Sbjct: 2 QLSNVTIRVRLWLGFGLLMVLMLAMAASGIARLGQLNAQMKDVIHDKYPKTVVAGDIVDQ 61 Query: 63 LDETARSMRNLLFMSTVDEIKGELNAITRAGAVIDETLKRFARDTPSAEGKRMLDAVNAA 122 L+ ARS+RN+L + +IK E I+ A I E L + + + + L + A Sbjct: 62 LNLIARSVRNILLLKREPQIKSEYERISGADRAIREDLAQLDKLLTDGDSRAALTTLVTA 121 Query: 123 RAQYTPVLDNFLAAVKDGQVEQARDLILPEIAPYQAAYFKALDNLIAFQGRGMEQAGQEA 182 + Y D LA V+ G E A D+++ E+ P Q+A A D LI Q M+ AG E Sbjct: 122 QNAYFEKRDKVLAMVQQGGKEGAIDMLIDEVRPVQSATMNAADALIRQQHEMMKTAGDEV 181 Query: 183 ERVSGAASVLMIAMATIASLLAVLVGYSVMRSITRPLNAAINIAERVAGGDLSVRVENSA 242 E A L++ +A A ++ + + RSIT PL+ A+ +AE VA GDL+ ++ Sbjct: 182 ESNYAQARTLLLLLAAAGLAAAAVIAWMITRSITAPLDRAVRVAETVAAGDLTSAIDAHG 241 Query: 243 HDETGKLLTALQGMRDGLLNAVTQVRDGSDAISVAAREIAAGNANLSARTELQASTLEET 302 DET LL AL+ M D LL+ V+QVR +D I+ A+ EIA GN +LS+RTE QA++LEET Sbjct: 242 RDETSMLLRALKKMNDRLLDIVSQVRVSTDTIATASSEIARGNLDLSSRTEEQAASLEET 301 Query: 303 ASSM----------LXNARQANQLVMNASQVATQGGDVVTQVVNTMREIKDSSRQIVEII 352 ASSM +A QA+QL +AS +A QGG+VV VV TM I SS++IV+II Sbjct: 302 ASSMEQLTATVKQNASHAGQASQLAGSASDIARQGGEVVGHVVETMGAIDASSKKIVDII 361 Query: 353 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRGLAQRSASAAKEIAGLIQNSV 412 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVR LAQRSA+AA EI LI +SV Sbjct: 362 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRALAQRSATAAHEIKALIADSV 421 Query: 413 KKVDAGSTLVDQAGNTMQDIVRSVKHVADIMTEISAASDEQSAGIRQVNDAIEQIDDMTQ 472 +V GS LV+QAG TM ++V+SV+ V +++ EIS+A EQSAGI QVN AI Q+D +TQ Sbjct: 422 ARVGNGSKLVEQAGRTMHEVVQSVQRVGEVVNEISSAGQEQSAGIEQVNLAIVQMDQVTQ 481 Query: 473 QNSALVEQAAAAAQSMHDQSMALGRAVSIFKIE-APGDIQTIGIGGSHAEPAPARISY 529 QN+ALVEQAAAAAQ++ DQ+ L R VSIFK+E A + + + A PA R+SY Sbjct: 482 QNAALVEQAAAAAQALQDQAAQLARMVSIFKLEDAASEPRRV------AAPASPRLSY 533 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 388 bits (997), Expect = e-109, Method: Compositional matrix adjust. Identities = 245/545 (44%), Positives = 337/545 (61%), Gaps = 25/545 (4%) Query: 3 KLSHLNIGQRLGLGFGVVILLMLGLTVIGVYRLNQINGNIRSIANDFYPRTVMANTVKGA 62 + ++L+I RL + F ++IL++ G+ +G+ RL IN + + ND Y + + NT+ G Sbjct: 2 RFNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGK 61 Query: 63 LDETARSMRNLLFMSTVDEIKGELNAITRAGAVIDETLKRFARDTPSAEGKRMLDAVNAA 122 +D+ A S+RN L M+ +I E I A + R + E K LD V Sbjct: 62 VDDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTET 121 Query: 123 RAQYTPVLDNFLAAVKDGQVEQARDLILPEIAPYQAAYFKALDNLIAFQGRGMEQAGQEA 182 R Y + G + A L+L E+ P Q YF LD L Q + M+Q+ +A Sbjct: 122 RKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQA 181 Query: 183 ERVSGAASVLMIAMATIASLLAVLVGYSVMRSITRPLNAAINIAERVAGGDLSVRVENSA 242 E ++M+ +A+ A + +++ RSITRPL+ A+ +AE VA GDL+ + + Sbjct: 182 EAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAHS 241 Query: 243 HDETGKLLTALQGMRDGLLNAVTQVRDGSDAISVAAREIAAGNANLSARTELQASTLEET 302 +DETG+LL AL M L V +VR G+D++ A+ +IA GN +LS+RTE QAS LEET Sbjct: 242 NDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEET 301 Query: 303 ASSM----------LXNARQANQLVMNASQVATQGGDVVTQVVNTMREIKDSSRQIVEII 352 ASS+ + R++++L NASQVA QGG V QVV TM I DSS +IV+II Sbjct: 302 ASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDII 361 Query: 353 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRGLAQRSASAAKEIAGLIQNSV 412 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRGLAQRSA+AAKEI LI +SV Sbjct: 362 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELINDSV 421 Query: 413 KKVDAGSTLVDQAGNTMQDIVRSVKHVADIMTEISAASDEQSAGIRQVNDAIEQIDDMTQ 472 +V +G+ LV QAG+TM +V S++ V+ I+ EISAA+ EQS GI QVN AI Q+D+ TQ Sbjct: 422 NQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMDNTTQ 481 Query: 473 QNSALVEQAAAAAQSMHDQSMALGRAVSIFKIEAPGDIQTIGIGGSHAEPAPARISYQEP 532 QN+ALVE+AAAAAQ++ DQ+ L V +F+++ +GGS PAP+ + Sbjct: 482 QNAALVEEAAAAAQALQDQAQRLLDLVGVFRLDE--------VGGSG--PAPSAV----- 526 Query: 533 APCRP 537 AP P Sbjct: 527 APVAP 531 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 384 bits (986), Expect = e-107, Method: Compositional matrix adjust. Identities = 253/551 (45%), Positives = 346/551 (62%), Gaps = 25/551 (4%) Query: 1 MKKLSHLNIGQRLGLGFGVVILLMLGLTVIGVYRLNQINGNIRSIANDFYPRTVMANTVK 60 M K+S + I RLG F +I++M + +GV L ++ I ND Y + + + Sbjct: 1 MMKISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQIT 60 Query: 61 GALDETARSMRNLLFMSTVDEIKGELNAITRAGAVIDETLKRFARDTPSAEGKRMLDAVN 120 ++ ARS+RN + +E K L+ + + + E L + T + G +L A+ Sbjct: 61 DKVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALK 120 Query: 121 AARAQYTPVLDNFLAAVKDGQVEQARDLILPEIAPYQAAYFKALDNLIAFQGRGMEQAGQ 180 ARA Y+ + + + ++A + + P Q YF A+ +++FQ M+++ + Sbjct: 121 QARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAE 180 Query: 181 EAERVSGAASVLMIAMATIASLLAVLVGYSVMRSITRPLNAAINIAERVAGGDLSVRVEN 240 A S +A +M+ ++ +A LL+ L + RSITRPLN A+ +A VA GDL+V++E Sbjct: 181 RASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEA 240 Query: 241 SAHDETGKLLTALQGMRDGLLNAVTQVRDGSDAISVAAREIAAGNANLSARTELQASTLE 300 ++ DETG LL AL+ M D L V +VR G+D I+ A+REIA GN +LS+RTE QA LE Sbjct: 241 TSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALE 300 Query: 301 ETASSM----------LXNARQANQLVMNASQVATQGGDVVTQVVNTMREIKDSSRQIVE 350 ETAS+M NARQAN L ASQVA QGG VV +VV TM +I D+SR+IV+ Sbjct: 301 ETASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVD 360 Query: 351 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRGLAQRSASAAKEIAGLIQN 410 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LAQRSA+AAKEI LI Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDA 420 Query: 411 SVKKVDAGSTLVDQAGNTMQDIVRSVKHVADIMTEISAASDEQSAGIRQVNDAIEQIDDM 470 SV++V++GS LV QAG TM+++V SVK V D++ EI+AAS EQS GI Q+N AI Q+D++ Sbjct: 421 SVERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDEV 480 Query: 471 TQQNSALVEQAAAAAQSMHDQSMALGRAVSIFKIEAPGDIQTIGIGGSHA-EPAPARISY 529 TQQN+ALVEQAAAAAQS+ +QS L V IF+ +G HA +P+P Sbjct: 481 TQQNAALVEQAAAAAQSLQEQSGRLSDTVGIFR-----------LGNDHAHQPSPLSAPV 529 Query: 530 Q---EPAPCRP 537 Q + AP RP Sbjct: 530 QRSVQVAPARP 540 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 365 bits (938), Expect = e-102, Method: Compositional matrix adjust. Identities = 231/513 (45%), Positives = 325/513 (63%), Gaps = 11/513 (2%) Query: 4 LSHLNIGQRLGLGFGVVILLMLGLTVIGVYRLNQINGNIRSIANDFYPRTVMANTVKGAL 63 +L + RL F ++ L+ ++V ++N IN I I ND Y + A ++ + Sbjct: 5 FKNLKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGV 64 Query: 64 DETARSMRNL-LFMSTVDEIKGELNAITRAGAVIDETLKRFARDTPSAEGKRMLDAVNAA 122 +E + +R + + + + + A E L + +A G++ A++ A Sbjct: 65 NEQIKYLRGIAIDIENRPANEKRFAQLEVAVKQTKEALDKITARQVTAVGQKKAKALDEA 124 Query: 123 RAQYTPVLDNFLAAVKDGQVEQARDLILPEIAPYQAAYFKALDNLIAFQGRGMEQAGQEA 182 R + +FLA VK G A + +L +I Q Y Q ++ G +A Sbjct: 125 RIAFDASKADFLALVKAGNSTSADEYVLRKITDTQNNYLALARAFADSQDAQLQAEGAKA 184 Query: 183 ERVSGAASVLMIAMATIASLLAVLVGYSVMRSITRPLNAAINIAERVAGGDLSVRVENSA 242 A + +A + A L A+++GY + RSI +PL A+ +AE VA GDL+ R+E + Sbjct: 185 VADGAMAIEVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRVAENVAAGDLTTRIEPHS 244 Query: 243 HDETGKLLTALQGMRDGLLNAVTQVRDGSDAISVAAREIAAGNANLSARTELQASTLEET 302 DETG+L+ AL+ M D L+ V+ VR G+++I+ A+ EIA+GN +LSARTE QA +LEET Sbjct: 245 RDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEET 304 Query: 303 ASSM----------LXNARQANQLVMNASQVATQGGDVVTQVVNTMREIKDSSRQIVEII 352 AS+M NARQANQ+ ++AS++A QGG +V +VV+TM EI SSR+IV+II Sbjct: 305 ASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEINQSSRKIVDII 364 Query: 353 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRGLAQRSASAAKEIAGLIQNSV 412 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA EVR LAQRSA+AAKEI LI +SV Sbjct: 365 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKALIADSV 424 Query: 413 KKVDAGSTLVDQAGNTMQDIVRSVKHVADIMTEISAASDEQSAGIRQVNDAIEQIDDMTQ 472 KV+ GS LV QAG TM+ +V SV+ V DI+ EISAAS EQS+GI ++N A+ Q+D+ TQ Sbjct: 425 GKVEGGSALVAQAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEEINRAVTQMDEGTQ 484 Query: 473 QNSALVEQAAAAAQSMHDQSMALGRAVSIFKIE 505 QN+ALVEQAAAAA+SM +Q++ L +++SIFK+E Sbjct: 485 QNAALVEQAAAAAKSMQEQAVRLTQSISIFKLE 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.338 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,386,476 Number of extensions: 51999 Number of successful extensions: 697 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 372 Number of HSP's successfully gapped: 71 Length of query: 541 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 451 Effective length of database: 1,193,720 Effective search space: 538367720 Effective search space used: 538367720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)