BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0085.p (415 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 626 e-180 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 402 e-113 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 91 2e-19 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 80 2e-16 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 626 bits (1614), Expect = e-180, Method: Compositional matrix adjust. Identities = 295/376 (78%), Positives = 332/376 (88%) Query: 1 VRALGSILTQEHVLASGTRVLMKQNKPDGFACVSCSWAKPADPHVFEFCENGAKATAWEI 60 V+ALGSIL QEHV+ASGTR+LM QNKP+GFACVSCSWAKPADPH+FEFCENGAKATAWEI Sbjct: 42 VKALGSILQQEHVIASGTRILMHQNKPEGFACVSCSWAKPADPHLFEFCENGAKATAWEI 101 Query: 61 TEKRATPEFFAQHTVTEFESWSDLELEAVGRVTHPMRYDDASDKYLPIPWDKAFDEICSA 120 T KRAT FFAQHTVTE E+W+DLELE+ GRVT PM+YD ASD+YLP+ W +AFD+I + Sbjct: 102 TRKRATAAFFAQHTVTEMEAWNDLELESSGRVTEPMKYDAASDRYLPVSWSEAFDDIAAQ 161 Query: 121 LRAADPEKVVFYTSGRASLETSYMYQLMARMYGNNNLPDSSNMCHESTSVALPKTIGVAV 180 LRA DP+KVVFY SGRASLETSY+YQLMARMYG NNLPDSSNMCHESTSVALP+TIGV V Sbjct: 162 LRAMDPKKVVFYASGRASLETSYLYQLMARMYGTNNLPDSSNMCHESTSVALPQTIGVPV 221 Query: 181 GTVTLDDFAATDCLLFFGHNTGTNAPRMLHSLEEVRKRGVPVITFNPLRERGLVSFVNPQ 240 GTVTLDDF TDC+LFFGHNTGTNAPRMLH LEEVRKRGVPVITFNPLRERGL FVNPQ Sbjct: 222 GTVTLDDFGQTDCILFFGHNTGTNAPRMLHPLEEVRKRGVPVITFNPLRERGLERFVNPQ 281 Query: 241 SPLEMLTPARTAISSQYVQIKIGGDTAAVMGMAKWLIEADDVARETRQPRLLDVGFIADH 300 SP EM+TPA T IS+QY+QIKIGGD+AA +GMAK ++E DD AR+ PRLLD+ FI +H Sbjct: 282 SPKEMVTPAHTDISTQYLQIKIGGDSAAAIGMAKRILERDDKARQEGLPRLLDLAFIEEH 341 Query: 301 TAGFEEFAAAARSADWNEIERCSGVSRAELEQAAQTYANARSTMLMYGMGVTQHREAVRT 360 T+GFE+FA A R+A W+E+ER +GVSRAELE AA TYA A MLMYGMGVTQHREAVRT Sbjct: 342 TSGFEQFADAVRAAPWDELERHAGVSRAELELAADTYAGAGRAMLMYGMGVTQHREAVRT 401 Query: 361 IHMLTNLLLLRGNVGR 376 IHMLTNLLL+RGN+G+ Sbjct: 402 IHMLTNLLLMRGNIGK 417 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 402 bits (1034), Expect = e-113, Method: Compositional matrix adjust. Identities = 204/405 (50%), Positives = 253/405 (62%), Gaps = 12/405 (2%) Query: 8 LTQEHVL-ASGTRVLMKQNKPDGFACVSCSWAKPADPHVFEFCENGAKATAWEITEKRAT 66 L +EHV G R L+ QN+PDGF C C+W FEFCENG KA A E T+KR T Sbjct: 27 LVKEHVADGRGLRTLLSQNQPDGFDCPGCAWPDREHTSTFEFCENGVKAVAAEATKKRVT 86 Query: 67 PEFFAQHTVTEFESWSDLELEAVGRVTHPMRYDDASDKYLPIPWDKAFDEICSALRA-AD 125 PEFFAQHTVTE SD ELE GR+THPM YD A+DKY I W AF + L A D Sbjct: 87 PEFFAQHTVTELMQQSDFELEEHGRLTHPMVYDAATDKYKKIEWADAFALMAKHLNALPD 146 Query: 126 PEKVVFYTSGRASLETSYMYQLMARMYGNNNLPDSSNMCHESTSVALPKTIGVAVGTVTL 185 P+ FY SGRAS E ++++QL R YG NN PD SNMCHE TSV LP T+G+ GTV L Sbjct: 147 PDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLL 206 Query: 186 DDFAATDCLLFFGHNTGTNAPRMLHSLEEVRKRGVPVITFNPLRERGLVSFVNPQSPLEM 245 +DF D LL FG N TN PRM+ L KRG ++ NPL+ERGL F +PQS +EM Sbjct: 207 EDFDHCDTLLLFGQNPATNHPRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEM 266 Query: 246 LTPARTAISSQYVQIKIGGDTAAVMGMAKWLIEADDVARETRQPRLLDVGFIADHTAGFE 305 LT T ISS ++ +GGD A + G+ K IE DD AR R++DV FI +HTAGFE Sbjct: 267 LTMGSTRISSMFIHPTLGGDLALIKGVIKRTIELDDAARAAGAERVIDVKFIEEHTAGFE 326 Query: 306 EFAAAARSADWNEIERCSGVSRAELEQAAQTYANARSTMLMYGMGVTQHREAVRTIHMLT 365 EFAA AR+ W++I SGVSR ++E+ AQ Y ++ + +GMG+TQH+ AV TI +L+ Sbjct: 327 EFAAQARAESWDDIVAESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKHAVATIQLLS 386 Query: 366 NLLLLRGNVGRRRRHLSYPRPFQRAGPAHRGHHRKAGDGAEGKTE 410 N+++LRGN+GR+ L P RGH GD G E Sbjct: 387 NMMMLRGNIGRQGAGLC---------PV-RGHSNVQGDRTVGIDE 421 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 72/292 (24%), Positives = 132/292 (45%), Gaps = 27/292 (9%) Query: 91 RVTHPMRYDDASDKYLPIPWDKAFDEICSALRAAD----PEKVVFYTSGRASLETSYMYQ 146 R+ +PM +D + + W++A S R + + TS R + E +Y+ Q Sbjct: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 Query: 147 LMARM-YGNNNLPDSSNMCHESTSVALPKTIGVAVGTVTLDDFAATDCLLFFGHNTGTNA 205 + R +GNNN+ + +CH T L +T+G + GT T + +D +L G N Sbjct: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 Query: 206 PRMLHSLEEVRKRGVPVITFNPLRERGLVSFVNPQSPLEMLTPARTAISSQY-VQIKIGG 264 P ++ ++G +I +P R +V +SP I + Y ++++ G Sbjct: 418 PVFASQIKRRVRQGAKLIVIDP-RTTEMV-----KSP---------HIQADYHLKLRPGT 462 Query: 265 DTAAVMGMAKWLIEADDVARETRQPRLLDVGFIADHTAGFEEFAAAARSADWNEIERCSG 324 +TA + +A +I ++ + E D+ D ++EF ++ + +G Sbjct: 463 NTAIITALAH-VILSEGLQDEAYIQERCDLQSYQD----WKEFVLREDNSP-EAMAAITG 516 Query: 325 VSRAELEQAAQTYANARSTMLMYGMGVTQHREAVRTIHMLTNLLLLRGNVGR 376 V + AA+ YA + + YG+GVT+H + T+ + NL + GNVGR Sbjct: 517 VPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGR 568 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 70/297 (23%), Positives = 116/297 (39%), Gaps = 40/297 (13%) Query: 90 GRVTHPMRYDDASDKYLPIPWDKAFDEICSALRAA----DPEKVVFYTSGRASLETSYM- 144 GR+ HP + DA + + WD+A D + S A P+ V Y SG+ E Y+ Sbjct: 68 GRLLHP-QLRDAQGQLQQLAWDQALDHVASKFSAIIAEHGPDAVALYVSGQLLTEDYYIA 126 Query: 145 YQLMARMYGNNNLPDSSNMCHESTSVALPKTIGVAVGTVTLDDFAATDCLLFFGHNTGTN 204 +LM G+ N+ +S +C S + G + V +D D ++ G N Sbjct: 127 NKLMKGYIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAWC 186 Query: 205 APRMLHSLEEVRKRGVPVITFNPLRERGLVSFVNPQSPLEMLTPARTA---ISSQYVQIK 261 P + + +++ P L ++ P RTA ++ ++ IK Sbjct: 187 HPILFQRIARLKE-------------------TRPALRLVVIDPRRTATCELADLHLPIK 227 Query: 262 IGGDTAAVMGMAKWLIEADDVARETRQPRLLDVGFIADHTAGFEEFAAAARS--ADWNEI 319 G D G+ +L L D FI HT GF+E A A + AD + Sbjct: 228 PGTDVWLFNGLMSYLAHRG----------LRDNAFIEAHTKGFDEALAVADADCADPATV 277 Query: 320 ERCSGVSRAELEQAAQTYANARSTMLMYGMGVTQHREAVRTIHMLTNLLLLRGNVGR 376 R + +L + +AN + + MGV Q ++ + N L G +G+ Sbjct: 278 ARLCKIDLKDLLAFYEAFANTEKVVTGFSMGVNQSSAGTDKVNSIINCHLATGRIGK 334 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,296,071 Number of extensions: 51000 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 4 Length of query: 415 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 327 Effective length of database: 1,203,190 Effective search space: 393443130 Effective search space used: 393443130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)