BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0084.p
         (360 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase...   518   e-148
gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta...   291   6e-80

>gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 790

 Score =  518 bits (1334), Expect = e-148,   Method: Compositional matrix adjust.
 Identities = 246/338 (72%), Positives = 281/338 (83%)

Query: 1   MVPKEKLKQQYCFDVPEKKGLNTVEACEEILKEKISAFFMLGGNFVRAIPDHGRMEPAWR 60
           +VP +KL+  Y F+ P +KGLNTVEACE+I   ++SAFFMLGGNFVRAIPDHG ME AWR
Sbjct: 445 LVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGGNFVRAIPDHGVMEAAWR 504

Query: 61  KIPLTVQVVTRLNRSSVIHGRRSYLLPCLGRIEIDQQAGGAQAVSVEDSTGCMHGSRGMV 120
           K+PLTVQVVT  NRS VIHG+ SY+LPCLGRIEID+Q GG QAVSVEDSTGCMHGSRG  
Sbjct: 505 KLPLTVQVVTHFNRSCVIHGQTSYVLPCLGRIEIDRQRGGEQAVSVEDSTGCMHGSRGRA 564

Query: 121 KPASEHLLSEVAIVAEIAKRILIPNPRVNWTAWRADYSRIRNAIAETYPEIFHDFNERMW 180
           +PAS HLLSE AIVAE+AKR+L  NP+V+W  W  DY+RIR+AI  TYP+IF DFN RMW
Sbjct: 565 EPASPHLLSEPAIVAELAKRLLPFNPQVDWDGWVGDYARIRDAIEATYPDIFRDFNARMW 624

Query: 181 TPGGFARPLAARERKWKTENGKANFITPDSFDEDPDMPADGPDVLRLMTTRGDSQFNTTV 240
            PGGFARPLAARER+WKT NG+ANF+TPD FD DPDMP D P+VLRLMTTRGDSQFNTTV
Sbjct: 625 NPGGFARPLAARERQWKTPNGRANFMTPDGFDPDPDMPGDAPEVLRLMTTRGDSQFNTTV 684

Query: 241 YGLDDRFRGVHGTRHMLLMHVADIERLGLAEGDMVTAATVSQDGIARSVDNLRVHAFDIP 300
           Y LDDRFRGV GTR +LLM++ D+++ GLA GD V A TVS DG+ R V +LRV  FDIP
Sbjct: 685 YSLDDRFRGVWGTRRVLLMNLRDLQQHGLAAGDFVCAETVSADGVERRVPHLRVEPFDIP 744

Query: 301 AGCIMGYFPELNPLIPLAHHARDSKVPAAKSIPVRLTP 338
            GC+MGYFPELN LIPL HHAR SKVPAAKS+P+RL P
Sbjct: 745 VGCVMGYFPELNRLIPLFHHARGSKVPAAKSVPIRLIP 782


>gi|300311015|ref|YP_003775107.1| anaerobic
           (selenocysteine-containing) formate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 775

 Score =  291 bits (745), Expect = 6e-80,   Method: Compositional matrix adjust.
 Identities = 149/339 (43%), Positives = 212/339 (62%), Gaps = 7/339 (2%)

Query: 5   EKLKQQYCFDVPEKKGLNTVEACEEILKEKISAFFMLGGNFVRAIPDHGRMEPAWRKIPL 64
           ++L+Q + F  P   G +TV +   +L+ +   F  LGGNF  A PD  R   A R   L
Sbjct: 429 DRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTFEAMRSCNL 488

Query: 65  TVQVVTRLNRSSVIHGRRSYLLPCLGRIEIDQQAGGAQAVSVEDSTGCMHGSRGMVKPAS 124
           TV + T+LNRS ++HG+ + +LP +GR EID Q  G Q V+VEDS   +H S G+ KPAS
Sbjct: 489 TVHITTKLNRSHLVHGKDALILPTMGRTEIDMQKSGPQGVTVEDSMSMVHVSYGINKPAS 548

Query: 125 EHLLSEVAIVAEIAKRILIP---NPRVNWTAWRADYSRIRNAIAETYPEIFHDFNERMWT 181
           EHL+SE AIVA +A+  +      P+++W  +  DYSRIR+AI + Y + F  +NER+  
Sbjct: 549 EHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEQVY-DAFKGYNERIAN 607

Query: 182 PGGFARPLAARERKWKTENGKANFITPDSFDEDPDMPA---DGPDVLRLMTTRGDSQFNT 238
           PGGF   +A+R+R WKT +GKANFI  D   + P   A    G  +L LMTTR   Q+NT
Sbjct: 608 PGGFHLGVASRDRVWKTASGKANFIVHDIPKDTPIHRARAIHGEKLLTLMTTRSHDQYNT 667

Query: 239 TVYGLDDRFRGVHGTRHMLLMHVADIERLGLAEGDMVTAATVSQDGIARSVDNLRVHAFD 298
           T+YG+DDR+RGV G R ++ ++ AD++ LGL +G+ V   +V  DG+ R  +   +  ++
Sbjct: 668 TIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRAEGFLLVEYE 727

Query: 299 IPAGCIMGYFPELNPLIPLAHHARDSKVPAAKSIPVRLT 337
           IP GCI  Y+PE NPL+PL   A  ++ P +KSIPV L+
Sbjct: 728 IPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLS 766


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,227,437
Number of extensions: 52880
Number of successful extensions: 131
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 128
Number of HSP's successfully gapped: 2
Length of query: 360
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 274
Effective length of database: 1,212,660
Effective search space: 332268840
Effective search space used: 332268840
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)