BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0070.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309655|ref|YP_003773747.1| hypothetical protein Hsero_0313... 150 5e-38 >gi|300309655|ref|YP_003773747.1| hypothetical protein Hsero_0313 [Herbaspirillum seropedicae SmR1] Length = 167 Score = 150 bits (379), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 72/167 (43%), Positives = 108/167 (64%), Gaps = 1/167 (0%) Query: 11 MTISFYNASVPVFKQGLNSLKGILAKAEAHATAKNIDADAFLKARLFPDMLNLTRQVQIA 70 M++S Y S+P+ GL++L +L KAE HA +D + L ARL+ DM L+ QVQ A Sbjct: 1 MSLSLYQVSIPMLIHGLDTLSTLLEKAEQHAAQHQLDPNELLTARLYEDMYALSGQVQRA 60 Query: 71 ADFAKGVPARLANVEVPPYEDNEASFADLQARLDKTLAFIEALKPEQFDGAAGREIVLRP 130 +D AK +RL+ V P + D+EASF DLQAR++KT+ F+E++ + DG R + ++ Sbjct: 61 SDTAKATASRLSGVAAPAFADDEASFDDLQARINKTIVFLESIPADAIDGQEERAVTMKL 120 Query: 131 GTPKEKRFTAEAYLLHYGVQHFLFHVTTAYDIFRHNGVEVGKSDYIG 177 +E+ TA+ YL +G+ +F FHVTTAY I RH GV++GK D++ Sbjct: 121 -RGEERTMTAQHYLTAFGLPNFFFHVTTAYGILRHKGVKLGKMDFLA 166 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 543,283 Number of extensions: 20729 Number of successful extensions: 58 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 1 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)