BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0052.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 196 5e-52 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 93 1e-20 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 88 3e-19 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 88 3e-19 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 86 2e-18 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 196 bits (499), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 117/133 (87%), Positives = 124/133 (93%), Gaps = 2/133 (1%) Query: 3 GIP--LTGGVLALAMRGIPLSISAAVGFIALSGVAVLNGLVMIAFIRTLREEGRSLDDAI 60 GIP LTGG+LALAMRGIP+SISAAVGFIALSGVAVLNGLVMI+FIR+LREEGR+LD AI Sbjct: 913 GIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAAI 972 Query: 61 FEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTLLTLLVLPL 120 EGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSST LTLLVLPL Sbjct: 973 HEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPL 1032 Query: 121 LYRLVHAGDSEDD 133 LY+L H D ED+ Sbjct: 1033 LYKLAHDKDEEDN 1045 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 92.8 bits (229), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 49/120 (40%), Positives = 78/120 (65%) Query: 6 LTGGVLALAMRGIPLSISAAVGFIALSGVAVLNGLVMIAFIRTLREEGRSLDDAIFEGAM 65 L G V A+ +R +P + VG I + G++ N ++++ F R LR EG L +A + ++ Sbjct: 902 LVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIVEFARQLRSEGMGLVEAAIKASV 961 Query: 66 TRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTLLTLLVLPLLYRLV 125 RLRP+LMT+L +LG VP+ LA+G AE QR + T V GG++S+T+L +L +P+ + LV Sbjct: 962 LRLRPILMTSLAFALGVVPLMLASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVLV 1021 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 88.2 bits (217), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 51/114 (44%), Positives = 69/114 (60%) Query: 13 LAMRGIPLSISAAVGFIALSGVAVLNGLVMIAFIRTLREEGRSLDDAIFEGAMTRLRPVL 72 L + G LS+ A G I GVA N ++++AF R REEG + A E TR+RPVL Sbjct: 928 LFITGTTLSVPALTGAIMTMGVATANSILIVAFARQRREEGATPLAAALEAGSTRIRPVL 987 Query: 73 MTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTLLTLLVLPLLYRLVH 126 MTAL +G +PMAL G GAE PL VIGG++ +T+ TL +P+++ VH Sbjct: 988 MTALAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLMLATVSTLFFVPVVFAGVH 1041 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 88.2 bits (217), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 48/116 (41%), Positives = 71/116 (61%), Gaps = 1/116 (0%) Query: 8 GGVLALAMRGIPLSISAAVGFIALSGVAVLNGLVMIAF-IRTLREEGRSLDDAIFEGAMT 66 G +LAL + G S+ A +G I L G+ N ++M+ F I R G S DAIFE Sbjct: 938 GALLALMVTGTDFSLIALIGVILLIGIVKKNAIMMVDFAIDAERNRGMSPRDAIFEACKL 997 Query: 67 RLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTLLTLLVLPLLY 122 R RP++MT + A LG +P+A+ G GAE+++PL ++GG++ S LLTL P++Y Sbjct: 998 RFRPIMMTTMAALLGAIPLAIGHGDGAELRQPLGISIVGGLVVSQLLTLYTTPVVY 1053 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 85.5 bits (210), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 40/119 (33%), Positives = 77/119 (64%) Query: 8 GGVLALAMRGIPLSISAAVGFIALSGVAVLNGLVMIAFIRTLREEGRSLDDAIFEGAMTR 67 G +L + +R +P + VG IA+ G++ N +++I F + L+ +G+ L +A E R Sbjct: 906 GALLGVTLRAMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGKGLIEATLEAVHLR 965 Query: 68 LRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTLLTLLVLPLLYRLVH 126 RP++MT+L LG +P+A+A+G G+ QR + T V+GG++++T+L + ++P+ + +V Sbjct: 966 FRPIIMTSLAFILGVLPLAIASGAGSASQRAIGTGVMGGMITATVLAVFLVPIFFVVVR 1024 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 447,759 Number of extensions: 15485 Number of successful extensions: 109 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 11 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)