BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0050.p (545 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 752 0.0 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 108 8e-25 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 74 3e-14 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 60 4e-10 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 59 9e-10 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 752 bits (1942), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/463 (77%), Positives = 413/463 (89%), Gaps = 2/463 (0%) Query: 1 MIVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALM 60 MIVYLPMFALTGVEGKMF PMAFTVVAAL+GAMILSVTFIPAAIALFIG+KV EKEN LM Sbjct: 460 MIVYLPMFALTGVEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALFIGEKVAEKENILM 519 Query: 61 RAAKRAYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQSLRIP 120 AKR Y P+L+ + NK VVL+ A A+I++C ++ +RLGSEFVPSLNEGD+AIQ+LRIP Sbjct: 520 LWAKRLYEPMLKTIMENKPVVLTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIP 579 Query: 121 GTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPQDQWP 180 GTSL+QS+EMQ+Q+E+TLK FPE+ERVFARTGTAEVASDPMPPNISDGYIMLKPQ+QWP Sbjct: 580 GTSLSQSLEMQKQIEKTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWP 639 Query: 181 KPRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMDVLND 240 +P+K+R+EL+ AIQ+ VA +PGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDM VLND Sbjct: 640 EPKKSREELLEAIQDEVAKIPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVLND 699 Query: 241 TAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAMAIGGKE 300 TA+KI+ L K+PGATEVK+EQ+TGLPMLTV IDREKTARYGLN+ D+QDTVA AIGGKE Sbjct: 700 TASKISAVLEKIPGATEVKIEQTTGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGGKE 759 Query: 301 AGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHNDG--KLSYIPLGEVADFDIAPG 358 AG +FQGDRRF I+VRLP+ R DL+AI+RLPI LP +G ++SY+PLGEVA IAPG Sbjct: 760 AGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIAPG 819 Query: 359 PNQVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFENLQSAT 418 PNQ+SRE+GKRR+VVSANVRGRDLG FV +A + + VK+P GYW WGGQFENLQSAT Sbjct: 820 PNQISREDGKRRIVVSANVRGRDLGGFVAEAERQLQSAVKVPAGYWTTWGGQFENLQSAT 879 Query: 419 QRLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGV 461 QRLQIV+PVALL+VFVLLFAMF+NVKDGLLVFTGIPFALTGG+ Sbjct: 880 QRLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGI 922 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 108 bits (271), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 114/456 (25%), Positives = 209/456 (45%), Gaps = 34/456 (7%) Query: 2 IVYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGD--KVDEKENAL 59 IV++PMF L+GV +F P+A VV A+ + +LS T +P + L +G +VD + +L Sbjct: 444 IVFVPMFFLSGVARFLFVPLAEAVVFAMAASYLLSRTLVPTLVMLLMGSHGQVDSAKQSL 503 Query: 60 MRAAKRAYAPLLQASLRNKLVVLS--------LAAAVIVLC---TLLGTRLGSEFVPSLN 108 ++ RA+ + +VLS A A + C LL LG +F P+++ Sbjct: 504 LQRLYRAFDNRFERVRHGYTLVLSSLLAHRRRFALAFLGFCLLSCLLYPFLGRDFFPNVD 563 Query: 109 EGDLAIQSLRIP-GTSLTQSVEMQQQLERTLKDNFP--EIERVFARTGTAEVASDPMPPN 165 G + + +R+P GT + ++ ++ER ++ P E+E + G + N Sbjct: 564 GGQIRLH-MRMPTGTRIEETARAADEVERVIRTLIPASELETILDNLGVPNSGINLSYSN 622 Query: 166 IS-----DGYIMLKPQDQWPKPRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELIS 220 DG I++ ++ +P + ++ A E PG + F QP + L Sbjct: 623 AGTIGTLDGEILMALKEGH-RPTEALVSMLRA--ELPRRFPGIEF-FFQPADIVTQILNF 678 Query: 221 GVRSDVAVKVFGDDMDVLNDTAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTAR 280 G+ + + V+ G +M+ AA++ + ++PGA + V Q P L +R+DR + + Sbjct: 679 GLPAAIDVQFTGPNMEANARLAAELTKQIRQIPGAVDAHVHQRLDGPSLDLRMDRTRLQQ 738 Query: 281 YGLNIGDVQDTVAMAIGGKEAG--ALFQGDRR---FDIIVRLPENERNDLDAIKRLPIPL 335 +GL+ +V + +A+ G A + ++I V+ P+ + + LDA+ +P+ Sbjct: 739 FGLSASNVGQNLLIALAGSSQTQPAFWLNPNNGVVYNIAVQSPQYQVDSLDALLNIPVSP 798 Query: 336 PHNDGKLSYIPLGEVADFDIAPGPNQVSRENGKRRVVVSANVRGRDLGSFVDDAAAGIAA 395 N LG + D SR N + V +V+G DL S +A + Sbjct: 799 GANASTTPPQLLGNLVDVHAGRQMAVASRYNIAPAIDVYVSVQGTDLASVAGKVSA-LVE 857 Query: 396 QV--KIPTGYWIAWGGQFENLQSATQRLQIVVPVAL 429 Q+ K+ G + GQ E +QS+ L + + +A+ Sbjct: 858 QIKPKLARGSQVVIRGQVETMQSSFTGLGVGLAMAI 893 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 97/471 (20%), Positives = 197/471 (41%), Gaps = 27/471 (5%) Query: 3 VYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALMRA 62 V++P+ + G+ G++F+ A TV + ++I+S+T P + F+ ++ ++ L + Sbjct: 457 VFIPLLLMGGIVGRLFREFAVTVTLTIAVSVIISLTLTPMLCSRFLKNEHGQRHGKLYQW 516 Query: 63 AKRAYAPLLQAS-------LRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQ 115 +R + +L ++++ + L A + L +L + F P + G + Sbjct: 517 FERCFDAMLAGYKRGLDVVMKHQFITLMSFIATLALTAVLFIFIPKGFFPQQDNGVIVGF 576 Query: 116 SLRIPGTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDGYIMLKP 175 + TS S M ER LK E+ R +++ N +I LKP Sbjct: 577 AESAQDTS---SAAMH---ERLLK--VAEVVRQDPDVIGFAMSAGSTTFNTGQFFIALKP 628 Query: 176 QDQWPKPRKTR-DELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDD 234 +DQ R T DE+IA ++ VA + G + + +S R+ + D Sbjct: 629 KDQG---RTTNADEIIARLRPKVARIEGVNLFMQASQDINVGGRLS--RTQYQYTITDTD 683 Query: 235 MDVLNDTAAKIAGTLGKVPGATEVKVEQSTGLPMLTVRIDREKTARYGLNIGDVQDTVAM 294 +D LN A ++ ++P T+V +Q + IDR + + +G++ + T+ Sbjct: 684 LDELNVWAPRMLERFRQLPQLTDVASDQQNQAAAAVITIDRARASSFGISPSLIDATIYD 743 Query: 295 AIGGKEAGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHNDGKLSYIPLGEVADFD 354 AIG ++ F + +I+ + + D +L + P + +PL D Sbjct: 744 AIGQRQVAQYFTQINSYRVILEVTPALQKDPALFDKLYLTSPLTGQQ---VPLSTFVKID 800 Query: 355 IAPGPN-QVSRENGKRRVVVSANV-RGRDLGSFVDDAAAGIAAQVKIPTGYWIAWGGQFE 412 + +S ++ V +S N+ +G LG V D Q+ +P + G + Sbjct: 801 TSKTSYLSISHQSQFPAVTISFNLAKGVSLGEAV-DVIQKTKDQMGVPQTLVGNFQGTAK 859 Query: 413 NLQSATQRLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGVYI 463 + ++ AL+ V+++L ++ + L + + +P A G + I Sbjct: 860 AFGDSLSSQPYLIAAALIAVYIVLGLLYESYIHPLTILSTLPSAGVGALLI 910 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 60.1 bits (144), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 105/483 (21%), Positives = 196/483 (40%), Gaps = 45/483 (9%) Query: 3 VYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFI----GDKVDEK--- 55 V++PM G G +++ + T+V+A+ ++++++T PA A + G V + Sbjct: 454 VFIPMAFFGGSTGVIYRQFSITIVSAMALSVMVALTLTPALCATLLKPHAGGHVQQGRFF 513 Query: 56 ---ENALMRAAKR---AYAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNE 109 + R+A R A LL+ R L+ +A A+ VL RL + F+P ++ Sbjct: 514 TWFNRSFDRSATRYQGGVAGLLKRGKRGLLMYALIAVAMAVLF----MRLPTSFLPEEDQ 569 Query: 110 GDLAIQSLRIP-GTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISD 168 G L Q +++P G + Q+ + + ++ + +E V A G A V + N + Sbjct: 570 GVLMAQ-IQLPTGATAEQTATVVKTVQEYFMNKPESVESVLAIVG-AGVGGNSQ--NGAR 625 Query: 169 GYIMLKPQDQWPKPRKTRDELIAAIQETVAALPGNSYEFSQPIQLRFNELISGVRSDVAV 228 +I LK +Q ++ EL+ ++ + G P +R SG D+ + Sbjct: 626 AFIRLKDWEQRTGAGQSASELVRRANADLSKIRGARVFLMNPPAVRGLGQSSGF--DLEL 683 Query: 229 K-VFGDDMDVL---NDTAAKIAGTLGKVPGATEVKVEQSTGL---PMLTVRIDREKTARY 281 K V G D L D ++A + + ++TGL P L V ID K Sbjct: 684 KDVGGVGHDALLQARDQFLQLARSNALLANV------RTTGLDDTPQLRVAIDDRKADAL 737 Query: 282 GLNIGDVQDTVAMAIGGKEAGALFQGDRRFDIIVRLPENERNDLDAIKRLPIPLPHNDGK 341 + D+ T+A A+GG R + V+ R D + R + + Sbjct: 738 SIATSDINATLATAMGGTYVNDFLSNGRVKKVYVQGDSAFRMQADDLNRWYV----RNAA 793 Query: 342 LSYIPLGEVADFDIAPGPNQVSRENGKRRV-VVSANVRGRDLGSFVDDAAAGIAAQVKIP 400 +P GP+ + R NG +V G G+ + + +A ++P Sbjct: 794 SQMVPFAAFMSSQWTYGPSLLERYNGVSSFEIVGEPAAGVSSGTAMAEVEKLVA---QLP 850 Query: 401 TGYWIAWGGQFENLQSATQRLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGG 460 G W G + + + ++ +++L VF+ L A++ + V +P + G Sbjct: 851 AGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYESWSVPFAVILAVPLGIIGT 910 Query: 461 VYI 463 V + Sbjct: 911 VLL 913 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 58.9 bits (141), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 100/475 (21%), Positives = 200/475 (42%), Gaps = 37/475 (7%) Query: 3 VYLPMFALTGVEGKMFQPMAFTVVAALLGAMILSVTFIPAAIALFIGDKVDEKENALMRA 62 V +P+ + V G++F A T+ ++L + ++S+T P A + + +E++ Sbjct: 445 VLIPLLFMGDVVGRLFHEFAVTLAVSILISAVVSLTLTPMMCARLLRHQPEEQQGWFYHK 504 Query: 63 AKRA-------YAPLLQASLRNKLVVLSLAAAVIVLCTLLGTRLGSEFVPSLNEGDLAIQ 115 +++ Y +LQ L + + L +AAA + L LL + F P + G + Sbjct: 505 SQQFFDRIIARYGVMLQWVLARQKLTLLVAAATLGLTVLLYVFVPKGFFPLQDTGVIQGI 564 Query: 116 SLRIPGTSLTQSVEMQQQLERTLKDNFPEIERVFARTGTAEVASDPMPPNISDG--YIML 173 S S + E QQQL + + + P +E + T+ + D ++ G I L Sbjct: 565 SEATQSISFSAMGEKQQQLAKVVLQD-PAVESL-----TSFIGVDGTNATLNSGRMLINL 618 Query: 174 KPQDQWPKPRKTRDELIAAIQETVAA-LPGNSYEFSQPIQ-LRFNELISGVRSDVAVKVF 231 KP D + ++I +Q +AA +PG + + QP+Q L + +S R+ + Sbjct: 619 KPHD---VREVSATDVIRRLQGKLAAEVPGITL-YMQPVQDLTIEDSVS--RTQYQFTLE 672 Query: 232 GDDMDVLNDTAAKIAGTLGKVPGATEVKVE-QSTGLPMLTVRIDREKTARYGLNIGDVQD 290 D VL+ K+ L + +V Q GL + IDR +R G+ + + Sbjct: 673 DADPAVLSTWVPKLIARLQQQSELADVATNLQDQGLQAY-IEIDRNAASRLGITTAAIDN 731 Query: 291 TVAMAIGGKEAGALFQGDRRFDIIVRL-PENERN--DLDAIKRLPIPLPHNDGKLSYIPL 347 + A G + ++ ++ +++ + PE + LD+I + GK +PL Sbjct: 732 ALYNAFGQRLISTIYTQSNQYRVVMEVKPEFQHGPAALDSIYL----VTSGGGK---VPL 784 Query: 348 GEVADFDIAPGPNQVSRENGKRRVVVSANV-RGRDLGSFVDDAAAGIAAQVKIPTGYWIA 406 +A P V+ VS N+ +G LG V A ++ +P Sbjct: 785 SSIATVTERTAPLVVNHIGQFPAATVSFNLAKGASLGEAVKAIKAA-EEEIGMPASTVTN 843 Query: 407 WGGQFENLQSATQRLQIVVPVALLLVFVLLFAMFNNVKDGLLVFTGIPFALTGGV 461 + G Q++ ++ A++ ++++L ++ + + + + +P A G + Sbjct: 844 FQGAALAFQASLSNTLWLILAAIVTMYIVLGVLYESYIHPITILSTLPSAGVGAL 898 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,570,129 Number of extensions: 63039 Number of successful extensions: 242 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 8 Length of query: 545 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 455 Effective length of database: 1,193,720 Effective search space: 543142600 Effective search space used: 543142600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)