BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0048.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309612|ref|YP_003773704.1| sugar ABC transporter permease ... 150 1e-37 gi|300313834|ref|YP_003777926.1| sugar ABC transporter permease ... 114 1e-26 gi|300313087|ref|YP_003777179.1| sn-glycerol-3-phosphate ABC tra... 97 2e-21 gi|300312637|ref|YP_003776729.1| sugar ABC transporter permease ... 93 3e-20 gi|300312389|ref|YP_003776481.1| sugar ABC transporter permease ... 68 1e-12 >gi|300309612|ref|YP_003773704.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 150 bits (379), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 95/294 (32%), Positives = 156/294 (53%), Gaps = 21/294 (7%) Query: 12 PNQERQPLTIGRVLIYVLLVLVALYYLAPLYVMITTSLKSLDEIRTSNLLALPMAPTTAA 71 P ++ +P I R L L + L +L P+ + TS++S DE+ N Sbjct: 5 PIEKWKP--INRALYRASLPVALLIWLLPMLAALVTSIRSNDELMAGNY----------- 51 Query: 72 WGKAWDSAC-----TGVDCGGLAPFFWNSIKMTIPAVLISTLIGSLNGYVLAHWRFRGSE 126 WG D A + + +FWNS +TIP+V+ + + ++ G+ L+ ++FRG+ Sbjct: 52 WGWPQDFAMLENYREALTASPMLHYFWNSCLITIPSVIGAISLAAMAGFALSTYQFRGNT 111 Query: 127 ALFAGLMVGCFIPFQVVILPMARLLGMFDLANTTPGLIFVHIVYGIAFTTLFFRNYYVTV 186 LFA + F+P Q++++P+ L L NT G++ HI F TLF RN+ + Sbjct: 112 VLFATFVACNFVPQQILMIPVRDLSLSLGLFNTITGMMLFHIAMQTGFCTLFLRNFIKQL 171 Query: 187 PEELVKAARIDGAGFFLTYRKIIFPLSLPIFMVCFIWQFTQIWNDFLFGVVF-GGSDAKP 245 P E+++AARI+GA + + +I+ PL P + FT +WND+ + +V G D P Sbjct: 172 PFEMIEAARIEGASEWTVFYRIVLPLIRPALAALAVLVFTFVWNDYFWALVLTQGDDVAP 231 Query: 246 VTVALNNLVNTSTGVTEYNVNMAAAIIAALPTLLVYILAGKYFVRGLTAGAVKG 299 +TV + L T T +N+ A +I+AALP+++++ + K FV GLT GA KG Sbjct: 232 ITVGVAALRGQWT--TAWNLVSAGSILAALPSVILFFVMQKQFVAGLTFGASKG 283 >gi|300313834|ref|YP_003777926.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 300 Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 78/278 (28%), Positives = 138/278 (49%), Gaps = 9/278 (3%) Query: 22 GRVLIYVLLVLVALYYLAPLYVMITTSLKSLDEIRTSNLLALPMAPTTAAWGKAWDSACT 81 G + ++ +L L A+ L P+ +++ S+K+ + I + ALP A T G + Sbjct: 31 GHLWVHTVLCLYAVIALFPIALILINSVKTRNAIFEGPM-ALPTAETLTFAG--FQKVLA 87 Query: 82 GVDCGGLAPFFWNSIKMTIPAVLISTLIGSLNGYVLAHWRFRGSEALFAGLMVGCFIPFQ 141 G +F NS+ +T+ A+ + L G++ + L +RF G+ AL + +G IP + Sbjct: 88 GTH---FLLYFGNSLAVTVAALFLIVLFGAMAAWALTEYRFFGNRALNFFIAIGIMIPIR 144 Query: 142 VVILPMARLLGMFDLANTTPGLIFVHIVYGIAFTTLFFRNYYVTVPEELVKAARIDGAGF 201 + + + +L+ DL NT L+ V+ G+ + + +P EL AAR DG G Sbjct: 145 LGTVSILQLVVALDLINTRTALVLVYTAQGLPLAVMILSEFMRQIPGELKDAARCDGVGE 204 Query: 202 FLTYRKIIFPLSLPIFMVCFIWQFTQIWNDFLFGVVFG-GSDAKPVTVALNNLVNTSTGV 260 + ++I PL P ++ WND F ++ G D + VT+ + + Sbjct: 205 LSIFFRVILPLLRPAIATVAVFTMIPAWNDLWFPLILAPGEDTRTVTLGVQQFIGQYA-- 262 Query: 261 TEYNVNMAAAIIAALPTLLVYILAGKYFVRGLTAGAVK 298 T++N +AA +A +P LL+Y+ + +RGLT+GAVK Sbjct: 263 TDWNSVLAALSMAVIPVLLLYMAFSRQLIRGLTSGAVK 300 >gi|300313087|ref|YP_003777179.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 70/255 (27%), Positives = 120/255 (47%), Gaps = 4/255 (1%) Query: 40 PLYVMITTSLKSLDEIRTSNLLALPMAPTTAAWGKA-WDSACTGVDCGGLAPFFWNSIKM 98 PLYV S +S ++ S L P + + A V + W S+ Sbjct: 26 PLYVAFVASTQSAEQSALSPLSLAPGGEFMNNYSTVLFKGAAGQVTAPPVLHMLWVSLAT 85 Query: 99 TIPAVLISTLIGSLNGYVLAHWRFRGSEALFAGLMVGCFIPFQVVILPMARLLGMFDLAN 158 + + I L+ + + ++RF G F + V +P +V I P +++ + N Sbjct: 86 ALIIAIGKISISMLSAFAIVYFRFPGRGLFFWMIFVTLMLPVEVRITPTYQVVSDLGMLN 145 Query: 159 TTPGLIFVHIVYGIAFTTLFFRNYYVTVPEELVKAARIDGAGFFLTYRKIIFPLSLPIFM 218 + GL I A T FR +++TVP+EL +AARIDGAG + +++PLS + Sbjct: 146 SYAGLSVPLIASATA--TFLFRQFFLTVPDELAEAARIDGAGPLRFLKDVLWPLSRTNVI 203 Query: 219 VCFIWQFTQIWNDFLFGVVFG-GSDAKPVTVALNNLVNTSTGVTEYNVNMAAAIIAALPT 277 F+ F WN +L+ ++ + P+ + + L++ E+N+ MA I+A LP Sbjct: 204 ALFVIMFIYGWNQYLWPLIIATDTSMYPIGIGIKTLISGGDSAVEWNLVMATMILAMLPP 263 Query: 278 LLVYILAGKYFVRGL 292 LV ++ K+FV+GL Sbjct: 264 GLVVVVMQKWFVKGL 278 >gi|300312637|ref|YP_003776729.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 86/303 (28%), Positives = 148/303 (48%), Gaps = 23/303 (7%) Query: 7 APKGNPNQERQPLTIGR------VLIYVLLVLVALYYLAPLYVMITTSLKSLDEI--RTS 58 A K + QE Q + + V IY+ L++ L P Y M+ T+ K +E+ ++ Sbjct: 2 AKKKDDQQETQGMDFLQSTPRKWVTIYIPLLIFLFVLLFPFYWMVITAFKPDNELLSQSG 61 Query: 59 NLLALPMAPTTAAWGKA-WDSACTGVDCGGLAPFFWNSIKMTIPAVLISTLIGSLNGYVL 117 N + +APT A + K +D+ + N++ +++ + S Y + Sbjct: 62 NPFWV-IAPTLAHFKKLLFDTQYPA--------WLLNTVIVSVVSTFASLAASVFAAYAI 112 Query: 118 AHWRFRGSEALFAGLMVGCFIPFQVVILPMARLLGMFDLANTTPGLIFVHIVYGIAFTTL 177 RF+GS+ + G+ + IP ++ +P+A ++ L +T LI + + I F T Sbjct: 113 ERLRFQGSKQVGLGIFLAYLIPPSILFIPLAAIVFKLGLFDTRWALILTYPTFLIPFCTW 172 Query: 178 FFRNYYVTVPEELVKAARIDGAGFFLTYRKIIFPLSLPIFMVCFIWQFTQIWNDFLFGVV 237 Y+ ++P EL + A IDGA + KII PL++P + I+ FT WN+F++ + Sbjct: 173 LLMGYFRSIPYELEECALIDGATRWEILVKIILPLAVPGLISAGIFAFTLSWNEFIYALT 232 Query: 238 F-GGSDAKPVTVAL-NNLVNTSTGVTEYNVNMAAAIIAALPTLLVYILAGKYFVRGLTAG 295 F S+ K V V + LV V + MA A++ +LP +VY +Y+V G+T G Sbjct: 233 FISSSEVKTVPVGIVTELVEGD--VYHWGALMAGALLGSLPVAVVYSFFVEYYVSGMT-G 289 Query: 296 AVK 298 AVK Sbjct: 290 AVK 292 >gi|300312389|ref|YP_003776481.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 276 Score = 67.8 bits (164), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 55/222 (24%), Positives = 112/222 (50%), Gaps = 11/222 (4%) Query: 83 VDCGGLAPF---FWNSIKMTIPAVLISTLIGSLNGYVLAHWRFRGSEALFAGLMVGCFIP 139 V+ APF F N+ + + ++ +L Y A + FRGS +F +++ + Sbjct: 60 VNAWQAAPFPRYFLNTFLLVTMILAAQLVLATLAAYAFARFTFRGSNVMFMLVLLQLMVM 119 Query: 140 FQVVILPMARLLGMFDLANT--TPGLIFVHIVYGIAFTTLFFRNYYVTVPEELVKAARID 197 ++I+ + + + L +T + GL + +GI R + TVP EL +AA ++ Sbjct: 120 PDILIVENYQTMNLLGLRDTILSIGLPYFASAFGI----FLLRQTFKTVPRELDEAATVE 175 Query: 198 GAGFFLTYRKIIFPLSLPIFMVCFIWQFTQIWNDFLFGVVFGGS-DAKPVTVALNNLVNT 256 GA + ++ PL+ PI++ + + WN+FL+ ++ S +++P+TV L +T Sbjct: 176 GASAWQILMQVYVPLARPIYIAFGLVSVSTHWNNFLWPLIVTNSVESRPLTVGLQVFSST 235 Query: 257 STGVTEYNVNMAAAIIAALPTLLVYILAGKYFVRGLTAGAVK 298 GV ++++ AA ++ + P LL ++L + FV+ ++ Sbjct: 236 DQGV-DWSIITAATLMTSAPLLLAFLLFQRQFVQSFMRAGIR 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 809,067 Number of extensions: 30714 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 9 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)