BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0047.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 121 8e-29 gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 113 2e-26 gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease ... 97 2e-21 gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease ... 95 7e-21 gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease ... 87 2e-18 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 121 bits (303), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 84/290 (28%), Positives = 143/290 (49%), Gaps = 5/290 (1%) Query: 28 KFAALADNWLPRLVLSPTIIFSLVFVYGFIALTGYLSLTNSRLMPNYT-FAGFDQYTRLF 86 K A WLP L+++P II +L+F + Y S+ Y+ F FD + LF Sbjct: 3 KRARFTSKWLPYLLVAPQIIITLLFFFWPAVQALYQSMLLQDAFGGYSEFVWFDNFKTLF 62 Query: 87 ALDRWWTAASNLGIFGGLFILLCLGIGLFMAILLDQKIRAEGALRAVYLYPMALSFIVTG 146 + + IF L L + L +A+ D+ R + ++P A+S +V G Sbjct: 63 GDPTYLESFRTTAIFSALVAFFGLALSLLLAVFADRVRRGASIYKTFLIWPYAVSPVVVG 122 Query: 147 AAWKWILNPGLGLEKMMHDWGFANFSFDWLVNSDMAIYTVVIAGVWQSSGFVMALFLAGL 206 W ++L+P LG+ + W +D+++N A+ +VIA +W+ + FLAGL Sbjct: 123 VLWMFLLSPSLGILSHVLSW--LGLPWDYMINGTHAMILIVIAAIWKQISYNFLFFLAGL 180 Query: 207 RGIDDAIIKAAMVDGASLPTIYRRIVIPALRP-VFFSVLLVLGHIAIKSFDLVMALTAGG 265 + I ++I+AA +DGA + IV P + P FF ++ + + +F +V A T GG Sbjct: 181 QSIPKSLIEAAAIDGAGPVKRFFTIVFPLISPTTFFLFVVNVVYAFFDTFVIVDATTHGG 240 Query: 266 PGTSSDLPAIFMYQFSFTRGQLGLGAASAVMMLA-TVLAVLVPLMYLETK 314 PG +++ ++ F G LG AA +V+++A +L +V Y+E K Sbjct: 241 PGKDTEILVYKVFSDGFKGGDLGGSAAQSVVLMALVILLTVVQFKYVEKK 290 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 113 bits (282), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 80/265 (30%), Positives = 134/265 (50%), Gaps = 6/265 (2%) Query: 42 LSPTIIFSLVFVYGFIALTGYLSLTNSRLMPNYTFAGFDQYTRLFALDRWWTAASNLGIF 101 L+P + +LV+V I T YLS + M F G Y LF ++TA N + Sbjct: 42 LAPACVMTLVYVLYPILYTIYLSFFSWDGMTEPRFVGLANYVELFHAPTFYTALKN-NVL 100 Query: 102 GGLFILLCLGIGLFMAILLDQKIRAEGALRAVYLYPMALSFIVTGAAWKWILNPGLGLEK 161 L LL +GL +A+ L+QK+ +++++ P LS +V G + W +P GL Sbjct: 101 WLLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMYSWFYDPSFGLLS 160 Query: 162 MMHDWGFANFSFDWLVNSDMAIYTVVIAGVWQSSGFVMALFLAGLRGIDDAIIKAAMVDG 221 ++ G L ++ A + ++ A +W + + M LFL GL ++ ++AA ++G Sbjct: 161 LLFGQGVPV-----LGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVEAARMEG 215 Query: 222 ASLPTIYRRIVIPALRPVFFSVLLVLGHIAIKSFDLVMALTAGGPGTSSDLPAIFMYQFS 281 A T+ R +++P LR F +V A++SFDL+ +T+GGP SS + A + Y + Sbjct: 216 AKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSGGPYESSTVLAYYAYDQA 275 Query: 282 FTRGQLGLGAASAVMMLATVLAVLV 306 + G AA AV + A +L +V Sbjct: 276 IKYYRQGYSAAVAVTLFAIMLVYIV 300 >gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 299 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 80/292 (27%), Positives = 143/292 (48%), Gaps = 12/292 (4%) Query: 35 NWLPRLVLSPTIIFSLVFVYGFIALTGYLSLTNSRLMPNYTFAGFDQYTRLFALDRWWTA 94 N L L ++P +I +VF+ + L +L T++++ +F G D +T L + Sbjct: 10 NVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYLAG-----DS 64 Query: 95 ASNLGIFGGLFI-----LLCLGIGLFMAILLDQKIRAEGALRAVYLYPMALSFIVTGAAW 149 + L +F +F +L +GL++AILL++ + + RA+ L P + ++ A+ Sbjct: 65 LAQLSLFNTVFYTVSASILKFMLGLWLAILLNKNVPLKTFFRAIVLLPWIVPTALSALAF 124 Query: 150 KWILNPGLG-LEKMMHDWGFANFSFDWLVNSDMAIYTVVIAGVWQSSGFVMALFLAGLRG 208 W+ + + +H G + D+L + A ++ V A VW+ FV LAGL+ Sbjct: 125 WWLYDAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISLLAGLQT 184 Query: 209 IDDAIIKAAMVDGASLPTIYRRIVIPALRPVFFSVLLVLGHIAIKSFDLVMALTAGGPGT 268 I ++ +AA +DGA+ +R + +P L P+ V+ F L+ LT GGP Sbjct: 185 ISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLTRGGPLN 244 Query: 269 SSDLPAIFMYQFSFTRGQLGLGAASAVMMLATVL-AVLVPLMYLETKGTRNG 319 ++ L A +Q + G LG GAA A M+ +L A++ L+ +G + G Sbjct: 245 ATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFSYFGLQRRGWQQG 296 >gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 95.1 bits (235), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 87/310 (28%), Positives = 143/310 (46%), Gaps = 16/310 (5%) Query: 1 MSNSTVSLSSTSSSAAGKPARRNGRSGKFAALADNWLPRLVLSPTIIFSLVFVYGFIALT 60 +S+++ + STS A+ + R +F + RL+ +P+++ L ++ +A+T Sbjct: 9 VSSTSPMMQSTSQPASRRQQTSPPRKERFNPI------RLMQTPSVLILLAWMIVPLAMT 62 Query: 61 GYLSLTNSRLM-PNYT-FAGFDQYTRLFALDRWWTAASNLGIFGGLFILLCLGIGLFMAI 118 Y S+ LM P+ T F G + Y L +W + N + G + + + G +A+ Sbjct: 63 IYFSVNRYNLMAPDQTGFVGLENYAFLLQDPAFWPSIGNTALLIGSVLAISVIGGTLLAV 122 Query: 119 LLDQKIRAEGALRAVYLYPMALSFIVTGAAWK-WILNPGLG-LEKMMHDWGFANFSFDWL 176 L DQ R G R + + P + V WK I++P G L +M G DWL Sbjct: 123 LFDQPFRGRGIARLLVIGPFFVMPTVAALIWKNMIMHPVYGILAYVMRHLGLE--PIDWL 180 Query: 177 VNSDMAIYTVVIAGVWQSSGFVMALFLAGLRGIDDAIIKAAMVDGASLPTIYRRIVIPAL 236 M +V+I WQ F + L L+ +D +AA +DGA I+ IV+P L Sbjct: 181 AEYPM--LSVIIIVAWQWIPFAFLILLTALQSLDTDQKEAAQLDGAGPVRIFFYIVLPHL 238 Query: 237 RPVFFSVLLVLGHIAIKSFDLVMALTAGGPGTSSDLPAIFMYQFSFTRGQLGLGAASAVM 296 + V+++ + F + TAGGPGT++ +Y S Q +G ASA Sbjct: 239 KRAITVVIMIETIFLLSVFAEIFTTTAGGPGTATTNLTFLVY--SIGLQQFDIGIASAGG 296 Query: 297 MLATVLAVLV 306 + A VLA +V Sbjct: 297 IFAVVLANIV 306 >gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 71/240 (29%), Positives = 114/240 (47%), Gaps = 12/240 (5%) Query: 75 TFAGFDQYTRLFALDRW----WTAASNLGIFGGLFILLCLGIGLFMAILLDQK-IRAEGA 129 +F G D Y + W W A N F + + L IGL +A L K +R Sbjct: 46 SFVGLDNYRTILFDPDWSAAFWNAMWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGART 105 Query: 130 LRAVYLYPMALSFIVTGAAWKWILNPGLGL-EKMMHDWGFANFSFDWLVNSDMAIYTVVI 188 R + P LS ++ G W+ IL+P G+ EK++ G A++ WL A+ T+ + Sbjct: 106 YRTLIFLPTLLSVVIIGFIWQLILSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSL 165 Query: 189 AGVWQSSGFVMALFLAGLRGIDDAIIKAAMVDGASLPTIYRRIVIPALRPVFFSVLLVLG 248 VWQ G M L A L + + +++AA +GAS I+ +I +P + P V ++ Sbjct: 166 ISVWQYVGIPMMLIYAALLAVPEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTF 225 Query: 249 HIAIKSFDLVMALTA--GGPGTSSDLPAIFMYQFSF-TRGQLG---LGAASAVMMLATVL 302 +FDL+ ++ GP +SDL + Y+ F + Q+G +GAA A +M +L Sbjct: 226 VANFNAFDLIYSVKGALAGPNYASDLLGTYFYRTFFGYQAQIGSPTMGAAVATLMFLVIL 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 872,863 Number of extensions: 32984 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 9 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)