BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0043.p (653 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312875|ref|YP_003776967.1| glucokinase [Herbaspirillum ser... 396 e-111 gi|300310430|ref|YP_003774522.1| transcription regulator protein... 197 3e-51 gi|300311078|ref|YP_003775170.1| transcription regulator protein... 111 2e-25 >gi|300312875|ref|YP_003776967.1| glucokinase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 396 bits (1018), Expect = e-111, Method: Compositional matrix adjust. Identities = 207/317 (65%), Positives = 247/317 (77%), Gaps = 1/317 (0%) Query: 19 DGPRLLADIGGTNARFALERAPGQIDTIQTLRCADYAEFSQAARAYLETNEHPAIHHAAI 78 DGPRLLADIGGTNARFALE PG+I+ +Q LR ADY EF+ A +AYL+ HP + HA + Sbjct: 14 DGPRLLADIGGTNARFALETGPGRIEQVQILRGADYGEFTDAVQAYLKLAGHPPVRHAVV 73 Query: 79 AIANPVQGDQIKMTNHHWAFSIEETRKLLGLDTLLVVNDFTALSMALPHLEQSHCVQIGG 138 AIANPVQGDQIKMTNH WAFSIE R+LLG + LLVVNDFTALSMA+P L Q+GG Sbjct: 74 AIANPVQGDQIKMTNHDWAFSIEAARQLLGFELLLVVNDFTALSMAVPQLRADELQQVGG 133 Query: 139 GQGLEGGVIGLVGAGTGLGVGGLIPSEGRWIALGSEGGHVSFSPADEKEAAILQYCWRTY 198 G G IGLVGAGTGLGVGGL+ ++G W+ L SEGGH +F+PAD +EAA+L Y W+ + Sbjct: 134 GAPKPGAPIGLVGAGTGLGVGGLLHADGHWLPLASEGGHAAFAPADPREAAVLAYAWQFH 193 Query: 199 KHVSAERLISGPGLEMIYSALCEINKIRNAEELTAPQIVDRALNQKDALCLEVVDVFCGM 258 +HVSAERL+SGPGLE+I+ AL I+ A EL+A QIV+ A DALC E + +FC M Sbjct: 194 EHVSAERLVSGPGLELIHRALLAIDG-HPAAELSAAQIVEGARQHGDALCQETLALFCSM 252 Query: 259 LGTLASNVAVTLGTLGGIYIGGGVVPHLGDYFARSAFRARFESKGRFDAYMQKIPTYVIT 318 LGT+A+++A+TLG LGGIYIG GVVPHLGDYFARS FRARFE+KGR + IPTYVIT Sbjct: 253 LGTVAADLALTLGALGGIYIGVGVVPHLGDYFARSPFRARFENKGRMSVLTKAIPTYVIT 312 Query: 319 APYPAFVGVAAILAERL 335 A YPAF GV+AILA+RL Sbjct: 313 AEYPAFTGVSAILADRL 329 >gi|300310430|ref|YP_003774522.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 286 Score = 197 bits (500), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 102/271 (37%), Positives = 167/271 (61%) Query: 343 FLERIRKARSQFSPAEERVAGLILAHPRAVMSEPISEIARRANVSQPTVIRFCRTMGCQG 402 L+ IR + S +E++VA ILA+P+ ++E ++ +A+ A VS+PTVIRFCR +G +G Sbjct: 4 LLDSIRTHINSLSKSEKKVAATILANPQLALAENLTALAKNAEVSEPTVIRFCRAIGYEG 63 Query: 403 LADFKLKLASGVTGTVPVAHSQVHIGDSALDVGVKVVDNTIAAMMEVRDSLNADTLSKVI 462 DFKLKLA + +P A + D A D+ K+ +I ++++R+ LN D + + Sbjct: 64 WHDFKLKLAQSLALALPGADETLAQDDLAADLVNKICSRSINTLLDLRNHLNPDAIQLAL 123 Query: 463 EVLSHATRVEFYGFGSCGLVAEDAQQKFFRLGIPSTAYTDPQLQEVSASLLKNTDVAVII 522 +VL+ A ++EFYG G+ G+VA DAQ KFFR G+P+ AY+DP + ++ASLLK D V I Sbjct: 124 DVLARANKIEFYGQGTSGIVANDAQHKFFRSGVPTVAYSDPHIHSIAASLLKAGDCVVAI 183 Query: 523 SNSGRLRHLATTVEVAVNSGATIIALAPSNSQLAKRADYTLAVEHDEGSMMHIPMVSRIL 582 S G L ++++A GA II L PS + LA+ A + ++ + P+ +R+ Sbjct: 184 SQRGGNAALLRSIQLARKGGADIIVLGPSGTPLAELATVLVPIDLSFNIDPYTPISARLA 243 Query: 583 LLLLVDVLAVGVSLNRSSPFAELQRQAKRGI 613 L+++D+LAVG++L R F + + A++ + Sbjct: 244 HLVVIDILAVGLALRRGPEFRKKMQNAQKSL 274 >gi|300311078|ref|YP_003775170.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 111 bits (278), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 92/302 (30%), Positives = 144/302 (47%), Gaps = 22/302 (7%) Query: 338 GNAVPFLERIRKARSQFSPAEERVAGLILAHPRAVMSEPISEIARRANVSQPTVIRFCRT 397 + + RI + S AE++VA IL A S I E+A +A VS +V RF + Sbjct: 2 AQTLDIISRITERSSTLRLAEQKVAEAILGDLHAAASASIGELADKAGVSVASVTRFAKA 61 Query: 398 MGCQGLADFKLKLASGVT---------GTVPVAHSQVHIGDSALDVGVKVVDNTIAAMME 448 MGC+ + +FK LA V TVP + + D VV I A++E Sbjct: 62 MGCRDVREFKFLLAQAVAVGQRFFDGAKTVP-SEQPPELAD--------VVYRDIHAVLE 112 Query: 449 V-RDSLNADTLSKVIEVLSHATRVEFYGFGS-CGLVAEDAQQKFFRLGIPSTAYTDPQLQ 506 + R LNAD L + +L A + +G G +++++A+ + RLG P +Y D LQ Sbjct: 113 LNRAMLNADMLMQAAHLLLGARMIYCFGMGGGSTMMSDEARYRLVRLGRPVASYHDAVLQ 172 Query: 507 EVSASLLKNTDVAVIISNSGRLRHLATTVEVAVNSGATIIALAPSNSQLAKRADYTLAVE 566 ++ A+ L DV ++ S +G + L + EVA G +IA+ S LAK AD L ++ Sbjct: 173 KMVAATLDKDDVLLVFSTTGSVPELLASCEVAREYGVKLIAITALGSPLAKLADVLLPIK 232 Query: 567 HDEGSMMHIPMVSRILLLLLVDVLAVGVSLNRSSPFAELQRQAKRGILAHI--ASTHDVL 624 E ++ P SR ++ +D+L + ++L E R+ K + AH H L Sbjct: 233 TLETDLIFKPSSSRYAMMATLDMLVLELALLHKESSQERLRRLKYVLDAHRGGGDLHQPL 292 Query: 625 GD 626 GD Sbjct: 293 GD 294 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,740,156 Number of extensions: 63464 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 3 Length of query: 653 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 562 Effective length of database: 1,188,985 Effective search space: 668209570 Effective search space used: 668209570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)