BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0041.p (372 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspir... 340 9e-95 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 143 3e-35 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 137 1e-33 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 133 2e-32 gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 128 7e-31 >gi|300310376|ref|YP_003774468.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 355 Score = 340 bits (873), Expect = 9e-95, Method: Compositional matrix adjust. Identities = 194/361 (53%), Positives = 250/361 (69%), Gaps = 14/361 (3%) Query: 20 MAGACASAQAQTN-VTVYGRIDAGINYQSN--QAGANGSRGSKWGIDGNEWGTSMFGFKG 76 M A AQAQT+ VT+YGR+ AG++ +N + +G+ G++ N+WGTSM G KG Sbjct: 1 MGAFSAQAQAQTSSVTIYGRVVAGVDIMTNVPKTDGSGTTGTRIRAASNQWGTSMIGVKG 60 Query: 77 TEDLGGGLKAIFTIESGFDASNGSVNSSSSNPGILWSRRSFVGLSGS-AGTLKVGKDLAI 135 TEDLGGG A F +ESGF ++ G +N + N L++RRS+VGLS + AG+LK+GK+L I Sbjct: 61 TEDLGGGTNAFFLLESGFGSTTGGLNGTDINGTSLFNRRSYVGLSSANAGSLKLGKNLFI 120 Query: 136 QSDPIWALDPTGQQALSTATLVKERNWPQTNNMVSYESPNFGGFTATVMHGFGEAAGSFT 195 +D +W LDPTGQQ + TA LV+ RNWP +N++ Y +P FGGF ATV GEAAGSF+ Sbjct: 121 NND-VWFLDPTGQQWIGTANLVRGRNWPGASNVIEYTTPTFGGFGATVQTSLGEAAGSFS 179 Query: 196 KGVPTATGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDTNGQYSTLFNSSKELTLGGTL 255 KG G + G +DG+SL Y ELR IYDV RD NG+Y+ ++++SKEL LGGT Sbjct: 180 KG-----GTSTKYGRKDGLSLVYQNNGLELRGIYDVIRDANGRYTDVYDTSKELILGGTY 234 Query: 256 TIDKLKLFAGYENLRAN--SVATGNPDRANHYWLGANYQITPALTLIGAAYHVNVNAGVG 313 IDKLK+F GYENLRA + A+G PDR NHYWLGANY +TP L+G+ +HVN N G G Sbjct: 235 KIDKLKMFLGYENLRAPDANTASGVPDRVNHYWLGANYDVTPQFQLVGSVFHVNANNGNG 294 Query: 314 SANLFVLGGNYSLSKRTLLYATVGTVRNGANSDFSVEYGG--GGIKGQNQQAFYTGISHS 371 SANLF+ G NY LSKRTLLY GT+RNG N+++S+E GG KG NQ Y GI+HS Sbjct: 295 SANLFMAGVNYFLSKRTLLYVGGGTMRNGNNANYSLEADSSTGGGKGTNQTGGYFGIAHS 354 Query: 372 F 372 F Sbjct: 355 F 355 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 118/359 (32%), Positives = 177/359 (49%), Gaps = 34/359 (9%) Query: 26 SAQAQTNVTVYGRIDAGINYQSNQAGANGSRGSKWGIDGNEWGTSMFGFKGTEDLGGGLK 85 S QAQ++VT+YG +D G+ Y +N AG NG+ GS + TS GF+GTEDLGGGLK Sbjct: 18 SVQAQSSVTIYGLVDTGVEYLNN-AGPNGA-GSVTRMTSGATNTSRIGFRGTEDLGGGLK 75 Query: 86 AIFTIESGFDASNGSVNSSSSNPGILWSRRSFVGLSGSAGTLKVGKDLAIQSDPIWALDP 145 A+F +E+GF NGS + ++ L++R+S VGL G G + G+ + D + DP Sbjct: 76 ALFQLENGFKLDNGSFDGDANQ---LFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDP 132 Query: 146 TGQQA------LSTATLVKERNWPQ-TNNMVSYESPNFGGFTATVMHGFGEAAGSFTKGV 198 G A + AT ++ P +N+V Y+ F G M+GFGE AG Sbjct: 133 MGYSANYSWATTAGATGNRKDGMPTGVSNLVKYQG-EFSGVKIGAMYGFGEVAGE----- 186 Query: 199 PTATGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDTNGQYSTLFNSSKELTLGGTLTID 258 + NG++ G+ L + + + A YD + ++ G + Sbjct: 187 -------TRNGAKYGVGLGWGTGPFSVVATYDRNNSATATNTPYDRATSAHLAAGYQPSE 239 Query: 259 KLKLFAGYENLRANSVATGNPD-RANHYWLGANYQITPALTLIGAAYHVNVNAGVGSAN- 316 K GY + S+ATG D R++ +W GA Y+ TPA+TLIGA Y+ N+ A+ Sbjct: 240 AWKFNLGYRYYK-KSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDADP 298 Query: 317 -LFVLGGNYSLSKRTLLYATV--GTVRNGANSDFSVEYGGGGIKGQNQQAFYTGISHSF 372 ++ L Y++SKRT LY + +NG S + G G +Q G+ H F Sbjct: 299 IMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAG---YGSSQFGMTLGMQHRF 354 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 132/397 (33%), Positives = 190/397 (47%), Gaps = 44/397 (11%) Query: 10 IAKPLAIAAVMAGACASAQAQTNVTVYGRIDAGINYQSN----QAGANGSRGSKWGIDGN 65 + K L AV+ +AQAQ++VT+YG +D GI Y S A N + GSK+GI+ Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG 60 Query: 66 EWGTSMFGFKGTEDLGGGLKAIFTIESGFDASNGSVNSS----SSNPGILWSRRSFVGLS 121 S GFKG EDLGGGL A+F +E+GF + +G + S SSN L+ R+S VGLS Sbjct: 61 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSN---LFRRKSVVGLS 117 Query: 122 GSAGTLKVGK--DLAIQSDPIWALDPTGQQALSTATLVKERNWPQTNNMVSYESPNFGGF 179 G GT+ +G+ D A A+ G ++ + + +TNN VSY + N GF Sbjct: 118 GGFGTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGF 177 Query: 180 TATVMHGFGEAAGSFTKGVPTATGVVSNNGSRDGISLAYVQ------PTYELRAIYDVQR 233 T +++GFGE AG + G G +NG G+ + Y Q P+ Sbjct: 178 TGNLIYGFGEQAGKTSAGQAYGIGGKYDNGPL-GLGINYYQSKAGSTPSDTSLIPAAGAT 236 Query: 234 DTNGQYSTLFNSS-KELTLGGTLTIDKLKLFAGYENLRAN-----SVAT---GNPDRANH 284 + N N++ K L + + +++A Y ++ + +VAT N +A+ Sbjct: 237 NANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATLANAQKADT 296 Query: 285 YWLGANYQITPALTLIGAAYH-------VNVNAGVGSANLFVLGGNYSLSKRTLLYATVG 337 Y +G Y ++P+L L+ A H VN G LG +Y LSKRT LYA V Sbjct: 297 YEIGTAYSLSPSLKLLAAVDHSRATFNGVNTK---GKLTQISLGADYFLSKRTDLYAFVS 353 Query: 338 TVRNGANSDFSVEYGGGGIKGQN--QQAFYTGISHSF 372 +R SD GG G + Q A TGI H F Sbjct: 354 NMR---ASDIQNPGVTGGATGSDASQTAIITGIRHKF 387 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 133 bits (335), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 124/379 (32%), Positives = 175/379 (46%), Gaps = 43/379 (11%) Query: 10 IAKPLAIAAVMAGACASAQAQTNVTVYGRIDAGINYQSNQAGANGSRGSKWGIDGNEWGT 69 + K L +AA++AG +A AQ++V++YG ID G+ Y S+ + G +D Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 70 SMFGFKGTEDLGGGLKAIFTIESGFDASNGSVNSSSSNPGILWSRRSFVGLSGSA-GTLK 128 S GFKGTEDLGGG+ A FT+E+GF A +GS+ G+L+ R+SFVGL + G+L Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQG----GLLFGRQSFVGLGSTQWGSLS 116 Query: 129 VGKDLAIQSD-----PIWALDPTG--------QQALSTATLVKERNWPQTNNMVSYESPN 175 VG+ ++ + AL P G A TA +TNN + Y S Sbjct: 117 VGRQYDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAK 176 Query: 176 FGGFTATVMHGFGEAAGSFTKGVPTATGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDT 235 FGGF+ M G GE AG+ G + +NG G +LAY D+ +D+ Sbjct: 177 FGGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSG-ALAYT----------DI-KDS 224 Query: 236 NGQYSTLFNSSKELTLGGTLTIDKLKLFAGYENLRANSVATGNPDRANHYWLGANYQITP 295 G +ST G I F ++ + + +A Y LG Y +TP Sbjct: 225 TGSFSTRIYGG-----GARYQIGAFTPFGVVTQVKNTNTSA----KATTYELGTVYALTP 275 Query: 296 ALTLIGAAYHVNVNAGVGSANLFVLGGNYSLSKRTLLYATVGTVRNGANSDFSVEYGGGG 355 L A N GVG+A V +Y +SKRT LY VG V + + GGG Sbjct: 276 LWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLY--VGAVYDRDKGYNAYPVFGGG 333 Query: 356 IKGQ--NQQAFYTGISHSF 372 I+ NQ A G+ H F Sbjct: 334 IQSATGNQTALRLGMRHRF 352 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 128 bits (322), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 110/359 (30%), Positives = 175/359 (48%), Gaps = 33/359 (9%) Query: 30 QTNVTVYGRIDAGINYQSNQAGANGSRGSKWGIDGNEWGTSMFGFKGTEDLGGGLKAIFT 89 Q++VT+YG ID G+ Y +N A ANG+ K + S GFKGTEDLGGGL+A+F Sbjct: 24 QSSVTIYGIIDTGVVYTTN-ANANGNSVFKMPSLTGSF-PSRIGFKGTEDLGGGLQAMFV 81 Query: 90 IESGFDASNGSVNSSSSNPGILWSRRSFVGLSGSAGTLKVGKDLAIQSDPIWALDPTGQQ 149 +ESGF G++ G L+ R+S+VGL S G L +G+ + + D G Sbjct: 82 LESGFAPDTGAMGQG----GRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPN 137 Query: 150 ALSTATLVKERNWPQTNNMVSYESPNFGGFTATVMHGFG----------------EAAGS 193 + ++ +++N + Y NF GFT + FG E AG+ Sbjct: 138 LFAIGSIDAYLPNARSDNAIGYLG-NFSGFTVGATYSFGRDAAGPAGPAATNCPGEVAGN 196 Query: 194 FTKGVPTATGVVSNNGSRDGISLAYVQPTYELRAIYDVQRDTNGQYSTLFNSSKELTLGG 253 +K T ++ +G G++ +Y +Y +NG S+ N+ + +TL G Sbjct: 197 -SKACRQVTALLGYDGKGWGVTSSY-------DILYGNTGASNGMTSS-DNTDQRVTLNG 247 Query: 254 TLTIDKLKLFAGYENLRANSVATGNPDRANHYWLGANYQITPALTLIGAAYHVNVNAGVG 313 + +K+ G + R + A N D ++ Y+LG +Y ++ AL L ++V Sbjct: 248 YFMLGDVKIGGGVVDRRTRAAANTNTD-SDLYYLGVSYPLSVALVLDAQVSRLDVKNSPN 306 Query: 314 SANLFVLGGNYSLSKRTLLYATVGTVRNGANSDFSVEYGGGGIKGQNQQAFYTGISHSF 372 L V Y+LSKRT +Y ++G ++NG S +++ GG G+NQ TGI H+F Sbjct: 307 DVTLSVARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTGIRHTF 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,177,326 Number of extensions: 51325 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 14 Length of query: 372 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 285 Effective length of database: 1,207,925 Effective search space: 344258625 Effective search space used: 344258625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)