BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0040.p
         (366 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311119|ref|YP_003775211.1| spermidine synthase [Herbaspiri...   100   3e-22

>gi|300311119|ref|YP_003775211.1| spermidine synthase
           [Herbaspirillum seropedicae SmR1]
          Length = 275

 Score = 99.8 bits (247), Expect = 3e-22,   Method: Compositional matrix adjust.
 Identities = 71/244 (29%), Positives = 110/244 (45%), Gaps = 26/244 (10%)

Query: 75  PKRVRFYLQALLEDADPPVSEEFIPMVVDRFDDLMSTSADGKPFIYSNGQSAYLHFNMLS 134
           P R R  L+A   DA  P   ++ P+ +   + +                  YLHF    
Sbjct: 18  PNRRRQQLEA---DASKPRKIKYAPVTLSELEGVR-----------------YLHFGTEW 57

Query: 135 VHSEMDMQAPFRLALGYTQTMMGFLFFHQDPVSIAMIGLGGGSLPKYCHQHLPNSLITVL 194
           V   M ++ P  + L Y Q MM ++ F++DP  I  +GLG G+L K+ ++  P + +T +
Sbjct: 58  VQGAMRIRKPDWIELEYAQQMMAWMLFNRDPRHIVQLGLGTGALTKFSYRQFPQAQVTAV 117

Query: 195 ENNSDVIAL-RDHFLIPQDDDRLSVQCVDGADFVKRHKEKY---DVLIVDGFDRVGQPAQ 250
           E N  VI +    F +P+ D+RL +   D  DF+  H   +   D L VD +D   +   
Sbjct: 118 ELNPAVITICHSMFKLPEPDERLMILESDAMDFIV-HAASHGIIDALQVDLYDATARGPV 176

Query: 251 LCSQDFYDNAYQMLDDGGIMVVNLIDIDYVGQVYVDRIRRSFDNAVIVIPVFDSMNQVVF 310
           L + +FY   Y  L  GG+M VNL          +  +  +FD  V+ +P     N V  
Sbjct: 177 LDTPEFYQACYDSLAAGGVMTVNLFGDHPSYAKNLRAMEHAFDT-VVSLPEVHDGNVVAL 235

Query: 311 ACKG 314
           A KG
Sbjct: 236 AFKG 239


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.140    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,062,240
Number of extensions: 42988
Number of successful extensions: 104
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 102
Number of HSP's successfully gapped: 1
Length of query: 366
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 280
Effective length of database: 1,212,660
Effective search space: 339544800
Effective search space used: 339544800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 131 (55.1 bits)