BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0036.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310202|ref|YP_003774294.1| DNA primase [Herbaspirillum ser... 310 7e-86 >gi|300310202|ref|YP_003774294.1| DNA primase [Herbaspirillum seropedicae SmR1] Length = 598 Score = 310 bits (794), Expect = 7e-86, Method: Compositional matrix adjust. Identities = 160/247 (64%), Positives = 196/247 (79%), Gaps = 4/247 (1%) Query: 1 VREKGAEAFEQQIKEAMPLSQFLIKEVVGDNDMTTPEGRARAQFDAKPMLQALPPSSLRL 60 VRE GA AFE+QI+EA+PLSQFL++E++G+ND+ TPEGRA AQF AKP+LQALP S+LRL Sbjct: 351 VREMGAPAFEEQIREALPLSQFLLREIIGENDLRTPEGRAHAQFAAKPLLQALPASALRL 410 Query: 61 QIVRSLAQVTQTTPAEIEALFELSRPVVQASTRRPPPRSKRMAPVGLERQIVRLLVAHPA 120 QIVRS+AQ TQ+TPAE+E LFEL +PV A R P RSKR APVGLERQI+RLLVAHP Sbjct: 411 QIVRSVAQATQSTPAELEVLFELEKPV--ARVRAAPARSKRTAPVGLERQIMRLLVAHPQ 468 Query: 121 LASDLDNDTLMVVGHHAPDHAQMVTQLVEACRSMGEQANFATLVELLRDGGADFESLIAE 180 LA+DLD L + APD A+M+T LV + MGEQANFA LVE LR+GG +F+S+IAE Sbjct: 469 LAADLDAGALEAIARIAPDQAEMLTHLVGVAQGMGEQANFAALVEHLREGGPEFDSMIAE 528 Query: 181 IAAETEAESEFDVVRQEMAGAVRQMKMRQVEDEMKQLSIAGLRNEIAQSRYRELMQRKEH 240 IAAE+ ESEFDVVR EM GA+RQ ++R +++EM+ L + LR E ++RYRELMQRKE Sbjct: 529 IAAES--ESEFDVVRAEMGGAIRQARIRALDEEMQMLVTSNLRTEEDRNRYRELMQRKEW 586 Query: 241 LKRLTGA 247 LKR GA Sbjct: 587 LKRQAGA 593 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 677,421 Number of extensions: 24487 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 1 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)