BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0025.p (108 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310194|ref|YP_003774286.1| thioredoxin 1 [Herbaspirillum s... 209 5e-56 gi|300312135|ref|YP_003776227.1| thioredoxin 1 [Herbaspirillum s... 117 2e-28 >gi|300310194|ref|YP_003774286.1| thioredoxin 1 [Herbaspirillum seropedicae SmR1] Length = 108 Score = 209 bits (531), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 104/108 (96%), Positives = 106/108 (98%) Query: 1 MSENIKHISDASFEADVLKSDKPVLVDFWAEWCGPCKAIAPILEEVAKEYDGKLQIAKLD 60 MS+ IKHISDASFEADVLKSDKPVLVDFWAEWCGPCKAIAPILEEVAKEY+GKLQIAKLD Sbjct: 1 MSDIIKHISDASFEADVLKSDKPVLVDFWAEWCGPCKAIAPILEEVAKEYEGKLQIAKLD 60 Query: 61 VDANQAIPAKFGIRGIPTLILFKNGAAAAQKVGALAKGQLTSFIDSNI 108 VDANQAIPAKFGIRGIPTLILFKNGAAAAQKVGALAKGQL SFIDSNI Sbjct: 61 VDANQAIPAKFGIRGIPTLILFKNGAAAAQKVGALAKGQLVSFIDSNI 108 >gi|300312135|ref|YP_003776227.1| thioredoxin 1 [Herbaspirillum seropedicae SmR1] Length = 110 Score = 117 bits (293), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 51/108 (47%), Positives = 78/108 (72%) Query: 1 MSENIKHISDASFEADVLKSDKPVLVDFWAEWCGPCKAIAPILEEVAKEYDGKLQIAKLD 60 MS NI ++DASFEADVL+S + VLVDFWA WC PCK +AP+L ++A+E+ L+IAK++ Sbjct: 1 MSSNIITVTDASFEADVLQSSQAVLVDFWAPWCMPCKMLAPLLADLAEEFHDTLRIAKVN 60 Query: 61 VDANQAIPAKFGIRGIPTLILFKNGAAAAQKVGALAKGQLTSFIDSNI 108 D N+ K+ +RG+P+++LF +G A+ VG +K +L +FI+ + Sbjct: 61 ADENKQHMEKYSVRGLPSILLFVDGVERARLVGMQSKTRLVTFIEDQL 108 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 262,609 Number of extensions: 8883 Number of successful extensions: 33 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 34 Number of HSP's successfully gapped: 2 Length of query: 108 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 36 Effective length of database: 1,278,950 Effective search space: 46042200 Effective search space used: 46042200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)