BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0021.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 630 0.0 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 610 e-176 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 551 e-158 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 369 e-103 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 99 7e-22 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 630 bits (1625), Expect = 0.0, Method: Compositional matrix adjust. Identities = 293/378 (77%), Positives = 335/378 (88%) Query: 56 QISGEVVKIGLITDLSGVYIDIDGQGGVEAIKMAIADFGGSVNGKKIELITADHQNKADI 115 QISG+V+KIG ITD+SGVY DIDGQGG EAIKMAIAD GG++NGKKIEL+ ADHQNKAD+ Sbjct: 25 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 84 Query: 116 AASKAREWFDQQGVDMLIGGTNSGTSLAMAKVAGEKKKTFIAVGGATARLTNEECTPYTI 175 AASKAREWFDQQGVDMLIGGTNSGT+LA AKVA EKKK ++ +G +ARLTNEEC+PYT+ Sbjct: 85 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 144 Query: 176 HYAYDTVALAKGTGSAIVKNGGKSWYFLTADYAFGASLEKDTSDIVKANGGTVTGAVKHP 235 HYAYDTVALAKGTGSA+VK GGKSW+FLTADYAFG SLE DT+++VKA+GGTV GAVKHP Sbjct: 145 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 204 Query: 236 LGASDFSSFMLQAQASKAQILGLANAGGDTINSIKAANEFGINKTMKLAGLLMFINDVHA 295 LGASDFSSF+LQAQ+SKAQI+GLANAGGD INS+KAANEFGI KT KLAGLL+FINDVH+ Sbjct: 205 LGASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHS 264 Query: 296 LTLQVTQGMYLTDGWYWDMNQETRDWSKRYFAKMKRMPSMLQAADYSATTQYLNAVKASG 355 LTL+ T+G+YLTD WYWD N+ETR ++KRYFA K+MPS LQAADYSA QYL VK G Sbjct: 265 LTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIG 324 Query: 356 TDDPDKVIAQMHKTKINDFFAKNGYIRPDGRMVHDMYLMEVKKPSESKYPWDYYKVVQTI 415 TDD DKV+A + K K ND + KNGY+R DGR++HDMYL +VK SESKYPWDYYK+VQTI Sbjct: 325 TDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTI 384 Query: 416 PAEQAWTTKAESKCSLWK 433 PAEQAWTTKAESKC+ WK Sbjct: 385 PAEQAWTTKAESKCAAWK 402 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 610 bits (1573), Expect = e-176, Method: Compositional matrix adjust. Identities = 289/403 (71%), Positives = 333/403 (82%), Gaps = 4/403 (0%) Query: 32 VKMTAIAATVVAACAA-GFAGPAAAQISGEVVKIGLITDLSGVYIDIDGQGGVEAIKMAI 90 ++ A++AT ACA G A +AQ +G+ +KIG ITD+SG Y D DGQGGVEAI+MAI Sbjct: 5 IRRAALSAT---ACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAI 61 Query: 91 ADFGGSVNGKKIELITADHQNKADIAASKAREWFDQQGVDMLIGGTNSGTSLAMAKVAGE 150 AD GG++NGKK+EL+ ADHQNKADIAA+KAREWFD+ GVDML+GG NS SLAM KVAGE Sbjct: 62 ADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGE 121 Query: 151 KKKTFIAVGGATARLTNEECTPYTIHYAYDTVALAKGTGSAIVKNGGKSWYFLTADYAFG 210 KKK FI+VG T R TNEEC YTI YAYDT ALA+GTG+AI + GGKSWYFLTADYAFG Sbjct: 122 KKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFG 181 Query: 211 ASLEKDTSDIVKANGGTVTGAVKHPLGASDFSSFMLQAQASKAQILGLANAGGDTINSIK 270 SLEKDTS++VK+NGG V GA + PL SDFSSF+LQAQ+SKAQILGLA AGGD IN+IK Sbjct: 182 TSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIK 241 Query: 271 AANEFGINKTMKLAGLLMFINDVHALTLQVTQGMYLTDGWYWDMNQETRDWSKRYFAKMK 330 AANEFG+NKTMKLAGLL+FIND H+L L +TQGMYLTDGWYWD+N +TR W+ RYF KMK Sbjct: 242 AANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFMKMK 301 Query: 331 RMPSMLQAADYSATTQYLNAVKASGTDDPDKVIAQMHKTKINDFFAKNGYIRPDGRMVHD 390 +MPSM QA D SA QYL AVKA G+ D DKV+ + K +INDFF NG +RPDGRMVHD Sbjct: 302 KMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVHD 361 Query: 391 MYLMEVKKPSESKYPWDYYKVVQTIPAEQAWTTKAESKCSLWK 433 MYLMEVKKPSESK PWDYYK+VQTIP EQA+ TKAESKC+LWK Sbjct: 362 MYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 551 bits (1419), Expect = e-158, Method: Compositional matrix adjust. Identities = 262/395 (66%), Positives = 323/395 (81%), Gaps = 3/395 (0%) Query: 41 VVAACA-AG-FAGPAAAQISGEVVKIGLITDLSGVYIDIDGQGGVEAIKMAIADFGGSVN 98 + AACA AG F+G A AQ+S + V+IG ITD+SG Y D DG GG+EAI+MA+ADFGG+V Sbjct: 10 LAAACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGGTVL 69 Query: 99 GKKIELITADHQNKADIAASKAREWFDQQGVDMLIGGTNSGTSLAMAKVAGEKKKTFIAV 158 GK IE+I+ADHQNKADIAA++AREW D++G+DMLIGG NS T+L+M KV EKK+ +I + Sbjct: 70 GKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINI 129 Query: 159 GGATARLTNEECTPYTIHYAYDTVALAKGTGSAIVKNGGKSWYFLTADYAFGASLEKDTS 218 G TARLTNEECTPYT+HY YDTVALAKGT SA++K GGKSW+FL ADYAFG SL DTS Sbjct: 130 GAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLANDTS 189 Query: 219 DIVKANGGTVTGAVKHPLGASDFSSFMLQAQASKAQILGLANAGGDTINSIKAANEFGIN 278 +VKA+GGTV G++KHP +SD SSF+LQAQASKAQILGLANAG DT+NSIKAA EFG+ Sbjct: 190 AVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGVT 249 Query: 279 KTMKLAGLLMFINDVHALTLQVTQGMYLTDGWYWDMNQETRDWSKRYFAKMKRMPSMLQA 338 KTMKL GLLM IND+HAL L +G+ +TD WYWD ++ +R +++R+F KMK+MPS QA Sbjct: 250 KTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMKKMPSTHQA 309 Query: 339 ADYSATTQYLNAVKASGTDDPDKVIAQMHKTKINDFFAKNGYIRPDGRMVHDMYLMEVKK 398 A YSATT YL AV+A GTDD KV+AQ+ KT+I+DF+ K G IR DGR +HDMYL +VK Sbjct: 310 AAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFYNK-GSIRADGRNIHDMYLYQVKS 368 Query: 399 PSESKYPWDYYKVVQTIPAEQAWTTKAESKCSLWK 433 P+ESK PWDY K+++TIP EQA+T+ A+SKCSL K Sbjct: 369 PAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 369 bits (946), Expect = e-103, Method: Compositional matrix adjust. Identities = 194/399 (48%), Positives = 263/399 (65%), Gaps = 5/399 (1%) Query: 38 AATVVAACAAGFAGPAAAQISGEVVKIGLITDLSGVYIDIDGQGGVEAIKMAIAD-FGGS 96 AA A A PA AQ VKIG++TD+SG Y + GQG V A KMA+ D Sbjct: 13 AAVACTVSLAATAIPAWAQ-DANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNE 71 Query: 97 VNGKKIELITADHQNKADIAASKAREWFDQQGVDMLIGGTNSGTSLAMAKVAGEKKKTFI 156 G KIE+++AD+QNK D+ ++AREW D+ V+ + TNS +LA+ K+ +K + Sbjct: 72 CKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKGGIAM 131 Query: 157 AVGGATARLTNEECTPYTIHYAYDTVALAKGTGSAIVKNGGKSWYFLTADYAFGASLEKD 216 G AT LT EC P H+ +DT + A G +A+ K G KSWYF+T DYAFG SLEKD Sbjct: 132 YSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKD 191 Query: 217 TSDIVKANGGTVTGAVKHPLGASDFSSFMLQAQASKAQILGLANAGGDTINSIKAANEFG 276 SD+VKANGGTV G+V+HPL ASDFSSF+LQAQASKAQ++GLA+ G D +N+IK A F Sbjct: 192 ASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFA 251 Query: 277 I-NKTMKLAGLLMFINDVHALTLQVTQGMYLTDGWYWDMNQETRDWSKRYFAKMKRM-PS 334 + K +L LL+F++DVHAL L+ QG+ TDG+YWD +++TR +SKR+ ++ K + P+ Sbjct: 252 VGGKDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSKRFESQFKNIKPT 311 Query: 335 MLQAADYSATTQYLNAVKASGTDDPDKVIAQMHKTKINDFFAKNGYIRPDGRMVHDMYLM 394 M+QA YS+ Q+L A A+ T D V +M + I D +N IRPDGR++HDMYL Sbjct: 312 MVQAGVYSSVYQFLKARAAAKTSDWKVVAQKMREIPIADAVMRNASIRPDGRVIHDMYLY 371 Query: 395 EVKKPSESKYPWDYYKVVQTIPAEQAWTTKAESKCSLWK 433 +VK P+ESK PWDY K++ IPA+QA+ ++ C L K Sbjct: 372 QVKTPAESKGPWDYLKLLSVIPAQQAF-RPMDAACPLVK 409 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 94/372 (25%), Positives = 161/372 (43%), Gaps = 20/372 (5%) Query: 60 EVVKIGLITDLSGVYIDIDGQGGVEAIKMAIADFGGSVNGKKIELITADHQNKADIAASK 119 E + G +SG + +G+ +K+A+ +G V GK + D + K A K Sbjct: 29 ESIAFGCPVPMSGPFA-ANGKFADLGMKLALQKYG-KVLGKSTSYVNLDTEGKPATAVRK 86 Query: 120 AREWFDQQGVDMLIGGTNSGTSLAMAKVAGEKKKTFIAVGGATARLTNEECTPYTIHYAY 179 +E ++G GG SG +LAM K A + FI GA +T +C T ++ Sbjct: 87 MQESI-EKGTRFFAGGILSGEALAMGKEADKAGAVFITTAGAD-EITGSDCNRATFRWSV 144 Query: 180 DTVALAKGTGSAIVKN--GGKSWYFLTADYAFGASLEKDTSDIVKANGGTVTGAVKHPLG 237 T + T +++ K WY +T Y FG L +I K G G H L Sbjct: 145 PTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHSLT 204 Query: 238 ASDFSSFMLQAQASKAQILGLANAGGDTINSIKAANEFGI--NKTMKLA---GLLMFIND 292 +FS ++ A A+K +L L N G + ++++ A FG+ N T+ +A GL F Sbjct: 205 EKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQF--- 261 Query: 293 VHALTLQVTQGMYLTDGWYWDMNQE-TRDWSKRYFAKMKRMPSMLQAADYSATTQYLNAV 351 L + G+Y ++ ++ +++ K +K P+ A Y T L+A+ Sbjct: 262 -ETLGADICDGVYFGAQYWHAIDAPFNKEFVKLVNDNLKIDPNYSLAGSYICTKIILDAI 320 Query: 352 KASGTDDPDKVIAQMHKTKINDFFAKNGYIRPDGRMVHDMYLMEVKKPSESKYPWDYYKV 411 + + DP VIA M K + D +++ + YL++ K ++ DY + Sbjct: 321 IKANSTDPKAVIAAMEGMKYEGLTGSEEIRKGDHQVIKNYYLLKGKAKAKMANKNDYADI 380 Query: 412 VQT----IPAEQ 419 V + +P E+ Sbjct: 381 VSSGKSFLPLEK 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,249,104 Number of extensions: 49113 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 13 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)