BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0016.p (686 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 786 0.0 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 495 e-141 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 394 e-110 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 346 4e-96 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 337 2e-93 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 786 bits (2029), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/681 (61%), Positives = 526/681 (77%), Gaps = 7/681 (1%) Query: 1 MAGSYNLLLICFSLFVATLASYTVLEIAERISSQETARYRRMWLAGGALVFGVGIWSTHF 60 M+GS++ LL+ F++ VA+L++Y LE++ER+ QE+ ++RR+WL+ GA+V G+G+W+T F Sbjct: 1 MSGSFHFLLLAFAVLVASLSAYAALEVSERMVMQES-QHRRLWLSVGAVVLGLGVWATLF 59 Query: 61 TGMLAFQLPINIGYNAFFTGISLLLCIGLSFYLLHRSTQRRLRWPRMLGDGVALGGGMAV 120 + A L + +G++ T ++LLLC+G +FYL+H S RR R+ G+++G + Sbjct: 60 LTVAALGLSMPVGFDIRLTVLALLLCLGSAFYLMHLSGLRRPHPSRLAIGGLSIGAAINA 119 Query: 121 VHWIGLHSMQIMPAVRYTPWLEILSVLATMAVAVLNIWIIYTLNARK------SDHFWRR 174 + L +MQ++PA+ Y L L++L T + V + + N RK + RR Sbjct: 120 AFHMALAAMQLLPALLYQQVLLGLALLLTELIGVFIVALFSASNGRKPKAGSLNSQQTRR 179 Query: 175 VIFAGMLGLGIFFMHCLNMAAANFSPDSISSTYASIDSDNLMSAVVLGTLFVLIAALIFS 234 V+ A ++GLG+ + A +P + S AS+ ++L++ +V + AL+ S Sbjct: 180 VLAALLVGLGLVAAATAGLRALVIAPHTQSLAVASLLREHLVNGIVFLAFLAMALALVIS 239 Query: 235 GAQKTKLLRKIVGRTNDKLLHFATHDVLTGLPNRALLAERIQHAIHVSKRSGLSFAVLFM 294 G Q+ ++L++IVGRTNDKLLHFATHDVLTGLPNRALLA+RIQHA+ V++R+G +FAVLFM Sbjct: 240 GVQRNQVLKRIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFM 299 Query: 295 DLDGFKAINDSLGHSVGDGLLIAVGQRIRACIRSEDMVSRIGGDEFVAVMGNLASPEVVE 354 DLDGFKAINDSLGH+VGDGLL+AV QRIR C+R EDMV+RIGGDEFV V+GNL+SP+VVE Sbjct: 300 DLDGFKAINDSLGHAVGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVE 359 Query: 355 NLAENILSVLRQDFRVDEATLRVTSSIGIAVYPNSGDTVEALMKNADAAMYEAKQNGRNT 414 L+ENIL+ LRQDF++D+ATLRVTSSIGIAVYPNSGD+V+ALMKNADAAMYEAKQ+GRNT Sbjct: 360 QLSENILAALRQDFQIDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNT 419 Query: 415 YRFFEPAMHASAMRHLTVRHALQLAVEHNQLSLHFQPKFEGRQRTLTGVEALLRWDHPEL 474 YRFFEPAMHASAMRHL VR ALQ A++ Q LHFQPK+ G + LTG+EALLRW HP+L Sbjct: 420 YRFFEPAMHASAMRHLQVRQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQL 479 Query: 475 GSMSPTEFIPIAERSGQIIPIGDWVLREVCAQIVRWDAAGMPPVKVALNLSPLQMRPDLV 534 G M+PTEFIPIAERSGQI+ IGDWVL+ VC QI RWDAAGM PVKVALNLSPLQMR DLV Sbjct: 480 GEMAPTEFIPIAERSGQILCIGDWVLQAVCEQIARWDAAGMKPVKVALNLSPLQMRTDLV 539 Query: 535 DSVTAIVSQAGIAPQRLMFEITETAAMKNVEKSARIITDLQQLGYDIAIDDFGAGYSSLA 594 + +V AGIAPQRLMFEITETAAMK+VE+S R+I +LQ LG+DIAIDDFG GYSSLA Sbjct: 540 ARIVELVGAAGIAPQRLMFEITETAAMKDVERSQRVIGELQALGFDIAIDDFGTGYSSLA 599 Query: 595 YLQQFHCRQLKIDKFFTSRLDQGGDSGPAIVSAIIALAHALNMEVVAEGVETEAQLAALQ 654 YL QFHCRQ+KID+FFTSRLD SG AIV+AIIALAHAL MEVVAEGVETE QL LQ Sbjct: 600 YLAQFHCRQIKIDRFFTSRLDGDDHSGRAIVAAIIALAHALQMEVVAEGVETEGQLRELQ 659 Query: 655 KLNCDQVQGFLLARPGAAGDV 675 L+CDQVQGFLL RP A V Sbjct: 660 LLHCDQVQGFLLGRPAEAPSV 680 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 495 bits (1274), Expect = e-141, Method: Compositional matrix adjust. Identities = 289/666 (43%), Positives = 411/666 (61%), Gaps = 19/666 (2%) Query: 17 ATLASYTVLEIAERI--SSQETARYRRMWLAGGALVFGVGIWSTHFTGMLAFQLPINIGY 74 A LASYT L++AERI ++ + R R WLAGGA+ G+GIWS HF GMLAF+LPI +GY Sbjct: 18 AILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWSMHFIGMLAFRLPIALGY 77 Query: 75 NAFFTGISLLLCIGLSFYLLHRSTQRRLRWPRMLGDGVALGGGMAVVHWIGLHSMQIMPA 134 +A T +SL++ I +S + L T+ L R+LG + +G G+A +H+ G+ ++++ P Sbjct: 78 DAGITALSLVIAIAVSGFALWIVTRPELPTRRLLGSALFMGIGIACMHYTGMAALRMQPG 137 Query: 135 VRYTPWLEILSVLATMAVAVLNIWIIYTLNARKSDHFWRRVIFAG---MLGLGIFFMHCL 191 + Y +L + SV +A + +WI ++L R + H R G ++G+ IF MH Sbjct: 138 IAYDLFLFLASVAIAIAASAAALWIAFSLR-RNTPHV--RAARGGASVVMGIAIFGMHYT 194 Query: 192 NMAAANFSPDSIS-STYASIDSDNLMSAVVLGTLFVLIAALIFS------GAQKTKLLRK 244 M AA+FS DSI S + L +++ TL L AL+ S ++ KL R Sbjct: 195 GMGAASFSRDSICLSAREGVAPGWLAVLIIVVTLAALTIALLTSVLDARLESRTAKLARS 254 Query: 245 IVGRTNDKLLHFATHDVLTGLPNRALLAERIQHAIHVSKRSGLSFAVLFMDLDGFKAIND 304 + N++L HD LT LPNR LL +R+ AI+ + R FA++FMDLDGFKAIND Sbjct: 255 LAA-ANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAIND 313 Query: 305 SLGHSVGDGLLIAVGQRIRACIRSEDMVSRIGGDEFVAVMGNLASPEVVENLAENILSVL 364 SLGH VGD LL+ V QR+ + R+ D V+R+GGDEFV ++ +L +PE +AE ++ + Sbjct: 314 SLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFV-ILVDLNTPEDAMGVAEKLVEAV 372 Query: 365 RQDFRVDEATLRVTSSIGIAVYPNSGDTVEALMKNADAAMYEAKQNGRNTYRFFEPAMHA 424 Q F+VD+ LRV++S+GIA+YP G T L+ NADAAMY K++GRN Y FFEP+M+A Sbjct: 373 NQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNA 432 Query: 425 SAMRHLTVRHALQLAVEHNQLSLHFQPKFEGRQRTLTGVEALLRWDHPELGSMSPTEFIP 484 +A + L L++A+E Q L +QPKF + G EALLRW+HP G ++P +FI Sbjct: 433 NAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIG 492 Query: 485 IAERSGQIIPIGDWVLREVCAQIVRWDAAGMPPVKVALNLSPLQM-RPDLVDSVTAIVSQ 543 +AERSG I+PIG WVL E C Q+ W A G +++NLS LQ P L++ V + + Sbjct: 493 LAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLER 552 Query: 544 AGIAPQRLMFEITETAAMKNVEKSARIITDLQQLGYDIAIDDFGAGYSSLAYLQQFHCRQ 603 + P L E+TE+ AM + E S + + + LG +I+IDDFG GYSSL YL++ + Sbjct: 553 HQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANE 612 Query: 604 LKIDKFFTSRLDQGGDSGPAIVSAIIALAHALNMEVVAEGVETEAQLAALQKLNCDQVQG 663 LKID+ F L Q G+ AIVSAI+AL LN+ +VAEGVET Q L CD +QG Sbjct: 613 LKIDRGFVRELRQDGEDA-AIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQG 671 Query: 664 FLLARP 669 +L+ RP Sbjct: 672 YLMGRP 677 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 394 bits (1011), Expect = e-110, Method: Compositional matrix adjust. Identities = 197/419 (47%), Positives = 276/419 (65%), Gaps = 2/419 (0%) Query: 252 KLLHFATHDVLTGLPNRALLAERIQHAIHVSKRSGLSFAVLFMDLDGFKAINDSLGHSVG 311 ++ H ATHD LTGLPNR +L E +Q A+ V+++ G AV F+DLD FK INDSLGH VG Sbjct: 261 EMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKFINDSLGHQVG 320 Query: 312 DGLLIAVGQRIRACIRSEDMVSRIGGDEFVAVMGNLASPEV-VENLAENILSVLRQDFRV 370 D LL V QR++AC+R DMV+R GGDEFV V+ N E + + + IL+ + + ++ Sbjct: 321 DELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQA 380 Query: 371 DEATLRVTSSIGIAVYPNSGDTVEALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHL 430 + +VT+SIG++ YP G VE+L+K+AD+AMY AK+ GRN ++FF P M + L Sbjct: 381 GDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNRL 440 Query: 431 TVRHALQLAVEHNQLSLHFQPKFEGRQRTLTGVEALLRWDHPELGSMSPTEFIPIAERSG 490 + L+ A++ ++ L++QPK + L G EAL+RW+ P+ G + P FIP AE +G Sbjct: 441 EMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAG 500 Query: 491 QIIPIGDWVLREVCAQIVRWDAAGMPPVKVALNLSPLQMRPDLVDSVTAIVSQAGIAPQR 550 I+PIG+WVLR C Q W AG+PP+ VA+NLSP Q+ L D V +++Q+G+A Sbjct: 501 LIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQSLPDFVAGVLAQSGLAASW 560 Query: 551 LMFEITETAAMKNVEKSARIITDLQQLGYDIAIDDFGAGYSSLAYLQQFHCRQLKIDKFF 610 L EITE MK+ EKS + L++LG I++DDFG GYSSL+YL++F LKIDK F Sbjct: 561 LELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSF 620 Query: 611 TSRLDQGGDSGPAIVSAIIALAHALNMEVVAEGVETEAQLAALQKLNCDQVQGFLLARP 669 + + DS AIV AII+LAH LN+ V+AEGVE E Q A L++ CD+VQG+ +P Sbjct: 621 VRDIVRDADSA-AIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKP 678 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 346 bits (887), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 178/428 (41%), Positives = 261/428 (60%), Gaps = 2/428 (0%) Query: 257 ATHDVLTGLPNRALLAERIQHAIHVSKRSGLSFAVLFMDLDGFKAINDSLGHSVGDGLLI 316 A+ D LTGL NR +L ++ AI V++R + V+F+DLD FK +ND+LGH GD LL Sbjct: 519 ASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLR 578 Query: 317 AVGQRIRACIRSEDMVSRIGGDEFVAVMGNLASPEVVENLAENILSVLRQDFRVDEATLR 376 V R+RA +R D V+R+GGDEF+ ++ + + I+ + + TL Sbjct: 579 TVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLF 638 Query: 377 VTSSIGIAVYPNSGDTVEALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLTVRHAL 436 +T S+G+A YPN G E L+K+AD AMY KQ GRN ++F+ PAM+ A+ L + L Sbjct: 639 MTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDL 698 Query: 437 QLAVEHNQLSLHFQPKFEGRQRTLTGVEALLRWDHPELGSMSPTEFIPIAERSGQIIPIG 496 + A++H + LH+QPK + + G EAL+RW HP+LG +SP FI +AE +G I+PIG Sbjct: 699 RNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPIG 758 Query: 497 DWVLREVCAQIVRWDAAGMPPVKVALNLSPLQ-MRPDLVDSVTAIVSQAGIAPQRLMFEI 555 WVLR C Q +W G + V++NLS Q ++ DLV S+ ++ G+ P+ L E+ Sbjct: 759 AWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELEL 818 Query: 556 TETAAMKNVEKSARIITDLQQLGYDIAIDDFGAGYSSLAYLQQFHCRQLKIDKFFTSRLD 615 TE+ M +VE + I+ L++LG ++IDDFG GYSSLAYL++F LKID+ F + Sbjct: 819 TESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDIT 878 Query: 616 QGGDSGPAIVSAIIALAHALNMEVVAEGVETEAQLAALQKLNCDQVQGFLLARPGAAGDV 675 D AI ++II+LA L + V+AEGVET+ QL L+ CD++QG+L + P A Sbjct: 879 IDADDA-AITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKF 937 Query: 676 QHFLGQGR 683 + L Q R Sbjct: 938 EQILRQDR 945 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 337 bits (864), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 178/430 (41%), Positives = 258/430 (60%), Gaps = 4/430 (0%) Query: 252 KLLHFATHDVLTGLPNRALLAERIQHAIHVSKRSGLSFAVLFMDLDGFKAINDSLGHSVG 311 KL H A +D L+GLPNR LL + +Q + +K+ AV+F+DLD FK +NDSLGHSVG Sbjct: 308 KLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVNDSLGHSVG 367 Query: 312 DGLLIAVGQRIRACIRSEDMVSRIGGDEFVAVMGNLASPEVVENLAENILSVLRQDFRVD 371 D LL+ QR+RA +R D+V+R+ GDEFVAV+ L + + + ++I++ L + F++ Sbjct: 368 DQLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAALAEPFQLG 427 Query: 372 EATLRVTSSIGIAVYPNSGDTVEALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLT 431 E +R+++S+GIA+YP+ DTV L+ AD AM+ AK GRN FF M + Sbjct: 428 EHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLAFFTEDMREKSFERRR 487 Query: 432 VRHALQLAVEHNQLSLHFQPKFEGRQRTLTGVEALLRWDHPELGSMSPTEFIPIAERSGQ 491 + + L A++ Q LHFQP E + + EAL+RW HP G +SP FIP+AE SG Sbjct: 488 LLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEESGL 547 Query: 492 IIPIGDWVLREVCAQIVRWDAAGMPPVK--VALNLSPLQMRPDLVDS-VTAIVSQAGIAP 548 I +G W+L+E VRW+A G P + +++NLSP+Q D+ V +S G P Sbjct: 548 IFDLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQLSLQGCQP 607 Query: 549 QRLMFEITETAAMKNVEKSARIITDLQQLGYDIAIDDFGAGYSSLAYLQQFHCRQLKIDK 608 + EITE + + E + +L+ G +A+DDFG GYS+++YL++F LKID+ Sbjct: 608 GLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKIDR 667 Query: 609 FFTSRLDQGGDSGPAIVSAIIALAHALNMEVVAEGVETEAQLAALQKLNCDQVQGFLLAR 668 F + DS AIV AII +AH L M+V+AEGVET Q L+ C+ VQG+L +R Sbjct: 668 SFVQDM-VDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSR 726 Query: 669 PGAAGDVQHF 678 P D +F Sbjct: 727 PLPEDDFLNF 736 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,140,713 Number of extensions: 92244 Number of successful extensions: 535 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 437 Number of HSP's successfully gapped: 45 Length of query: 686 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 594 Effective length of database: 1,184,250 Effective search space: 703444500 Effective search space used: 703444500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)