BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0005.p (187 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310179|ref|YP_003774271.1| TRAP-type C4-dicarboxylate tran... 336 6e-94 >gi|300310179|ref|YP_003774271.1| TRAP-type C4-dicarboxylate transport system small permease [Herbaspirillum seropedicae SmR1] Length = 186 Score = 336 bits (862), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 158/186 (84%), Positives = 174/186 (93%) Query: 2 LKFLDHLEEWLIAFLMAAATVIIFVAVLHRYASGLPIPWLQDELIQINTSWAQELCIFMF 61 +K LDHLEEWLIAFLMAAAT IIFVAV+HRYASGL IPWLQD+LIQIN SWAQELCIFMF Sbjct: 1 MKLLDHLEEWLIAFLMAAATFIIFVAVVHRYASGLDIPWLQDQLIQINMSWAQELCIFMF 60 Query: 62 VWMAKFGAAYGVRTGIHVGVDVLINRMNTPWRNKFVVLGLLAGALFTGIVGTLGAQFVWE 121 VWMAKFGAAYGVRTGIHVGVDVLINR+NTPWRNKFV+ GL AGALFTGIVGT GA+FV++ Sbjct: 61 VWMAKFGAAYGVRTGIHVGVDVLINRLNTPWRNKFVLFGLFAGALFTGIVGTFGAEFVYD 120 Query: 122 ISQHSSTSEVLEIPMWMVYMAVPLGSYLMCFRFLQVCWAFIKTGELPKHDHAYVEGLDEE 181 I+QH STSEV+E+PMW+VY+AVPLGSYLMCFRFLQV W F +TGELPKHDHA+VEGLDEE Sbjct: 121 IAQHDSTSEVMEVPMWIVYLAVPLGSYLMCFRFLQVAWNFHRTGELPKHDHAHVEGLDEE 180 Query: 182 IAGGKA 187 +GGKA Sbjct: 181 TSGGKA 186 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.141 0.465 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 633,688 Number of extensions: 25717 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 2 Length of query: 187 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 107 Effective length of database: 1,241,070 Effective search space: 132794490 Effective search space used: 132794490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)