BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0002.p (93 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310178|ref|YP_003774270.1| TRAP-type C4-dicarboxylate tran... 139 5e-35 gi|300312105|ref|YP_003776197.1| TRAP-type C4-dicarboxylate-bind... 115 4e-28 >gi|300310178|ref|YP_003774270.1| TRAP-type C4-dicarboxylate transport system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 334 Score = 139 bits (350), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 66/81 (81%), Positives = 73/81 (90%) Query: 1 MKLKSLVFALAAATIIAGNAFAQAPIIIKFSHVVANDTPKGKGAERFKELAEKATKGRVK 60 MKLKS++ A+ AA I++ NAFAQ PI+IKFSHVVANDTPKGK AERFKELAEKATKGRVK Sbjct: 1 MKLKSILLAMTAAAIVSTNAFAQQPIVIKFSHVVANDTPKGKAAERFKELAEKATKGRVK 60 Query: 61 VEVYPNSTLYKDKEELEALXC 81 +EVYPNSTLYKDKEELEAL Sbjct: 61 IEVYPNSTLYKDKEELEALQL 81 >gi|300312105|ref|YP_003776197.1| TRAP-type C4-dicarboxylate-binding periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 344 Score = 115 bits (289), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 55/57 (96%), Positives = 56/57 (98%) Query: 23 QAPIIIKFSHVVANDTPKGKGAERFKELAEKATKGRVKVEVYPNSTLYKDKEELEAL 79 Q PIIIKFSHVVANDTPKGKGAERFKELAEKAT+GRVKVEVYPNSTLYKDKEELEAL Sbjct: 31 QQPIIIKFSHVVANDTPKGKGAERFKELAEKATQGRVKVEVYPNSTLYKDKEELEAL 87 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.130 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 201,063 Number of extensions: 5667 Number of successful extensions: 23 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 23 Number of HSP's successfully gapped: 2 Length of query: 93 Length of database: 1,619,870 Length adjustment: 63 Effective length of query: 30 Effective length of database: 1,321,565 Effective search space: 39646950 Effective search space used: 39646950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)