BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0001.p (670 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 1072 0.0 gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains ... 153 5e-38 gi|300310677|ref|YP_003774769.1| two component transmembrane sen... 135 1e-32 gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sens... 111 1e-25 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 103 3e-23 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 1072 bits (2772), Expect = 0.0, Method: Compositional matrix adjust. Identities = 522/653 (79%), Positives = 579/653 (88%) Query: 10 SHLLSPGRRHLLRWVMPILLVLLFLMTLIWLPRQAQQMESSERQEQLIADSLWVEQAIRF 69 S L + GR+ +RW++P LLV LFL TLIWLPRQAQQME+SER EQLIADSLWVEQAIRF Sbjct: 17 SQLRASGRKTTMRWLIPFLLVCLFLATLIWLPRQAQQMEASERLEQLIADSLWVEQAIRF 76 Query: 70 QLSRDDESMRLIAREIVNEHLKKDQFRSRLNALLRNNREFYRVTWLSAEGKPIASTDDFP 129 QLSRDDESMR+I REI N L ++ FRSRLNALLRNNREFYRVTW +AEG+P+ASTDDFP Sbjct: 77 QLSRDDESMRIIGREITNGRLHQEHFRSRLNALLRNNREFYRVTWFNAEGRPVASTDDFP 136 Query: 130 VPRHELSTPSQQAEEQARTIRRPIYSAPAVPPGIAEPVVMDYHIPLFRENHYLGSVVASY 189 + R +LS S E+AR +RRP+YS PAVPPGIAEP++MDYH+PLF +NHY+GS+VASY Sbjct: 137 ITRADLSKSSLMTGERARQMRRPLYSPPAVPPGIAEPMLMDYHVPLFHDNHYVGSLVASY 196 Query: 190 SVARVLNEMVPWWFAQDNEIVLTDTDDKVVARRTAGGPGLNVYTHRRELELPGLTLRLHT 249 +VAR+LNEMVPWWFAQDNEI LTDTDD VVARRT+GGPGLNVYTHRRELELPGLTL LHT Sbjct: 197 AVARILNEMVPWWFAQDNEISLTDTDDNVVARRTSGGPGLNVYTHRRELELPGLTLVLHT 256 Query: 250 NSTKSAPKLLPNLLVGSVIVLSLGLLWSLWALWRDINRRMTAEEALVHEVSFRSAMENSL 309 NSTKSAPKLLPNLLVGSVIVLSLGLLWSLWALWRDINRR AE+AL++E FR+AMENSL Sbjct: 257 NSTKSAPKLLPNLLVGSVIVLSLGLLWSLWALWRDINRRTKAEKALLNEAVFRAAMENSL 316 Query: 310 VTGMRARDLEGRITYVNPAFCTMVGIPADELVGKIPPMPYWAPEAMDEYQERFSKVLAGR 369 VTGMRARDLEGRITYVNPAFC MVG A+ELVGKIPPMPYWAPEAMDEYQERFS VLAGR Sbjct: 317 VTGMRARDLEGRITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGR 376 Query: 370 VTPQFETIFQRKDGERFPVLIFESPLVDQNGKQTGWMGSILDVTDRKRAEDLNRMQEEKL 429 TPQFETIFQR+DGERFPVLIFESPLVDQ+GKQTGWMGSILD+++RKRAEDLNR QEEKL Sbjct: 377 ATPQFETIFQRRDGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKL 436 Query: 430 QTGARLASMGEIASTLAHELNQPLAAISSYTTGALNMMQSGAIDPASLQPALEKINTQAQ 489 Q ARLASMGEIASTLAHELNQPLAAISSYTTGALN+MQ G DP +LQPALEKI+ QAQ Sbjct: 437 QASARLASMGEIASTLAHELNQPLAAISSYTTGALNLMQQGQADPKALQPALEKIHAQAQ 496 Query: 490 RAGNVIRSVHQFVKKREPTRQPVSIKGIVDNIAPLVELQAQQFLVVVQTAIPADLPNVLG 549 RAG+VIRSVHQFVKKREP RQ V ++ ++DN+APL+ELQAQQF VV+Q +P LP VLG Sbjct: 497 RAGHVIRSVHQFVKKREPARQRVQMRQLIDNVAPLIELQAQQFFVVLQLQVPRSLPPVLG 556 Query: 550 DAVLLEQVLLNLTRNAIQAMAAIAPERRILRIAAALDRQTSSQQSVVVSVIDQGHGIPPE 609 D +LLEQV+LNLTRN IQAMAA+ RRILR+AA + R +SV VSV DQGHGIP + Sbjct: 557 DQILLEQVILNLTRNGIQAMAAVPSARRILRVAAEVTRNEDGAESVTVSVTDQGHGIPDD 616 Query: 610 VAAALFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYANNPAGGTIFRFSLPS 662 VAA LFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTY +NPAGGTIFRFSLP+ Sbjct: 617 VAARLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPA 669 >gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains containing sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 524 Score = 153 bits (386), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 103/346 (29%), Positives = 173/346 (50%), Gaps = 9/346 (2%) Query: 322 ITYVNPAFCTMVGI-PADELVGKIPPMPYWAPEAMDEYQERFSKVLAGRVTPQFETIFQR 380 + Y + C + G+ P + P+ E E + +A T + E R Sbjct: 185 LMYCSDELCRIYGLAPGRNCITYEEFAAIMHPDDRQEVVETVNAAVAMGGTIRVEHRICR 244 Query: 381 KDGERFPVLIFESPLVDQNGKQTGWMGSILDVTDRKRAEDLNRMQEEKLQTGARLASMGE 440 +D L + ++G T ++G+ D+T R++ E R + ++ +R ++G+ Sbjct: 245 QDTGEVRYLSGIGKPIWRDGTFTEYVGTATDITVRRQNEYAIRAAQIDMERVSRANTVGQ 304 Query: 441 IASTLAHELNQPLAAISSYTTGALNMMQSGAIDPASLQPALEKINTQAQRAGNVIRSVHQ 500 + +++AHE+NQPL +I + +L + D + L I + QRAG +I + Sbjct: 305 LTASIAHEINQPLMSIVANAGASLRWLDRPVPDLEHARTGLRDIIAEGQRAGGIISGLRD 364 Query: 501 FVKKREPTRQPVSIKGIVDNIAPL--VELQAQQFLVVVQTAIPADLPNVLGDAVLLEQVL 558 + P + V++ ++ +I L EL+ ++ +++ + PAD VLGD V L+QV+ Sbjct: 365 LTRNAAPVCERVNLHAVIRHILTLSRSELERREVSLLL-SLTPAD-AFVLGDQVQLQQVM 422 Query: 559 LNLTRNAIQAMAAIAPERRILRIAAALDRQTSSQQSVVVSVIDQGHGIPPEVAAALFSPF 618 LNL NAI+AM+++ R+L I A+ + +Q + V V D G GI PE +F F Sbjct: 423 LNLVVNAIEAMSSMPEHARVLSITAS----SCDEQRIEVKVQDSGEGIAPEAMRRVFDAF 478 Query: 619 FSTKAEGMGMGLNICRTTIEFHGGTLTYANNPAGGTIFRFSLPSLP 664 ++TK GMGMGL ICR+ IE H G L A G +F F LP +P Sbjct: 479 YTTKDSGMGMGLAICRSIIENHRGRLRVAAAQPHGALFSFDLPRVP 524 >gi|300310677|ref|YP_003774769.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 103/350 (29%), Positives = 175/350 (50%), Gaps = 18/350 (5%) Query: 319 EGRITYVNPAFCTMVGIPADELVGKIPPMPY---WAPEAMDEYQERFSKVLAGRVTPQFE 375 +G I Y++ A ++G+ G+ PP + P++ +E QE + L ++ Sbjct: 164 DGGIEYLSDAGRKLLGVRP----GQNPPKNFVEQCLPDSQEELQELLTLALPDAISFPLR 219 Query: 376 TIFQRKDG-ERFPVLIFESPLVDQNGKQTGWMGSILDVTDRKRAEDLNRMQEEKLQTGAR 434 RKDG R+ + ++ + K G + D+TD+ AE+ R + L+ AR Sbjct: 220 YRIARKDGLVRWHHAVCRYIEIENDLK--GHLLHFHDITDQVEAEEARRRDQAALEYLAR 277 Query: 435 LASMGEIASTLAHELNQPLAAISSYTTGA-LNMMQSGAIDPASLQP--ALEKINTQAQRA 491 +MGE+A+ +AHEL+QPLAAI ++ G+ L + S A+ + Q LE Q A Sbjct: 278 HNAMGEMATAIAHELSQPLAAIRNFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHA 337 Query: 492 GNVIRSVHQFVKKREPTRQPVSIKGIVDNIAPLVELQAQQFLVVVQTAIPADLPNVLGDA 551 +I+SV ++V K E + + + ++ + +E++A + V ++ + V + Sbjct: 338 AAIIKSVREYVVKLEQSEGLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEK 397 Query: 552 VLLEQVLLNLTRNAIQAMAAIAPERRILRIAAALDRQTSSQQSVVVSVIDQGHGIPPEVA 611 VL+ QV+LNL NAI+ M+ +R R+ +L R+ Q +++V D+G GI Sbjct: 398 VLIGQVILNLAFNAIEEMSHFPENQR--RVCISLARK---DQQALLTVADEGRGIDAGHR 452 Query: 612 AALFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYANNPAGGTIFRFSLP 661 LF FFS+K G G+GL +C+ I H G + GT F F+LP Sbjct: 453 ERLFDGFFSSKVSGNGIGLALCKNIIARHRGDIWAQPAQVRGTRFCFTLP 502 >gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 600 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 80/253 (31%), Positives = 127/253 (50%), Gaps = 12/253 (4%) Query: 416 KRAEDLNRMQEEKLQTGARLASMGEIASTLAHELNQPLAAISSYTTGALNMMQSGAIDPA 475 ++ E L R + +L +LA +G++A+ + HELNQPL AI ++ A+ + G A Sbjct: 357 QQTESLLRTTQNELVQAGKLAMLGQMAAGVTHELNQPLTAIRAFADNAVKFLARG--QSA 414 Query: 476 SLQPALEKINTQAQRAGNVIRSVHQFVKKREPTRQPVSIKGIVDNIAPLVELQAQQFLVV 535 LE I++ + G +I + F +K V + ++ + L+ + Q+ Sbjct: 415 QAVENLEYISSASATMGKIIAQLKGFARKSGEAVACVDLSQAIEASSLLLASECQKLGAT 474 Query: 536 VQTAIPADLPNVLGDAVLLEQVLLNLTRNAIQAMAAIAPERRILRIAAALDRQTSSQQSV 595 + I A V GD V EQVL+NL RNA+ A+ E RIA L+ + Sbjct: 475 ISIDIRA-AACVTGDVVRTEQVLVNLLRNALDAV----EEAEHKRIAVVLE---VAGGYA 526 Query: 596 VVSVIDQGHGIPPEVAAALFSPFFSTK--AEGMGMGLNICRTTIEFHGGTLTYANNPAGG 653 +V + D G GI EVA LF PFF+TK ++G+G+GL I + ++ G L+ N+ GG Sbjct: 527 LVRIRDSGGGIADEVAPHLFEPFFTTKSSSKGLGLGLAISSSIVQAMNGQLSAHNHEEGG 586 Query: 654 TIFRFSLPSLPVQ 666 F LP L ++ Sbjct: 587 AEFVVRLPLLKME 599 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 103 bits (258), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 94/351 (26%), Positives = 157/351 (44%), Gaps = 21/351 (5%) Query: 322 ITYVNPAFCTMVGIPADELVGKIPPMPYWAPEAMDEYQERFSKVLAGRVTPQFETIFQRK 381 I +VN AF M G + E+VGK PE + +A R E + RK Sbjct: 68 IVFVNNAFINMTGYSSAEVVGKNCRF-LQGPETDRSVVAEVRRAVAERREIATELLNYRK 126 Query: 382 DGERFPVLIFESPLVDQNGKQTGWMGSILDVTDRKRAEDLNRMQEEKLQTGARLASMGEI 441 +G F +F SP+ DQ G + S LD++ R+ AE + L ++ ++G++ Sbjct: 127 NGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRDAE-------QALGQARKMEALGQL 179 Query: 442 ASTLAHELNQPLAAISSYTTGALNMMQSGAIDPASLQPALEKINTQAQRAGNVIRSVHQF 501 +AH+ N L I+ Y ++S D A + + + I + +A + + + F Sbjct: 180 TGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLAF 239 Query: 502 VKKREPTRQPVSIKGIVDNIAPLVELQAQQFLVVVQTAIPADLPNVLGDAVLLEQVLLNL 561 +K+ + +++ + + LV + + VQT + L N D +E LLN+ Sbjct: 240 ARKQRLEGRTINLNSLTEGFTDLVS-RTLGGDITVQTELARGLWNCRLDPTQMEVALLNV 298 Query: 562 TRNAIQAMAAIAPERRILRIA----AALDR--QTSSQQSVVVS--VIDQGHGIPPEVAAA 613 NA AM R +R A A DR Q ++ + VS V D G GI P++ Sbjct: 299 LINARDAMNGRGRVR--IRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPR 356 Query: 614 LFSPFFSTKAE--GMGMGLNICRTTIEFHGGTLTYANNPAGGTIFRFSLPS 662 + PFF+TK E G G+GL++ ++ GG++ + GGT P+ Sbjct: 357 VMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPA 407 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,902,022 Number of extensions: 74845 Number of successful extensions: 406 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 385 Number of HSP's successfully gapped: 26 Length of query: 670 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 578 Effective length of database: 1,184,250 Effective search space: 684496500 Effective search space used: 684496500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)