BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0152.p (664 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 1236 0.0 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 565 e-162 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 119 1e-27 gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 108 1e-24 gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni... 75 1e-14 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 1236 bits (3197), Expect = 0.0, Method: Compositional matrix adjust. Identities = 586/664 (88%), Positives = 621/664 (93%) Query: 1 MTSTLPTDKMANAIRALAMDAVQKANSGHPGAPMGMAEIAVALWAKHYRHNPTNPQWANR 60 M STLPTDKMANAIRALAMDAVQKANSGHPG PMGMAEIAVALWAKHYRHNP NP WANR Sbjct: 1 MISTLPTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANR 60 Query: 61 DRFVLSNGHGSMLQYALLHLTGYDLPMEEIKNFRQLHSKTAGHPEVHITPGVETTTGPLG 120 DRFVLSNGHGSMLQYALLHLTGYDL ME+IK+FRQLHSKTAGHPEVHITPGVETTTGPLG Sbjct: 61 DRFVLSNGHGSMLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLG 120 Query: 121 QGITNAVGMALAEKLLAAEFNKPDLAVVDHYTYAFVGDGCLMEGISHEACSLAGTLRLSK 180 QGITNAVGMALAEKLLAAEFNKP LAVVDHYTYAFVGDGCLMEGISHEACSLAG L LSK Sbjct: 121 QGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSK 180 Query: 181 LIVLYDDNGISIDGKVEGWFKDDTPKRFEAYGWNVIPAVDGHNVEAVNSAIHQAKLADKP 240 LIVLYDDNGISIDG VEGWFKDDTPKRFEAYGWNVIPAVDGH+VEAV++AIHQAKLADKP Sbjct: 181 LIVLYDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKLADKP 240 Query: 241 TLICCKTVIGKGSPNLAGTDKVHGAALGDKEVAAVREALGWTSAPFEIPADIYSAWDGKA 300 TLICC+TVIGKGSPNLAGTDKVHGAALGDKE+AAVREALGWTSAPFEIPAD+YSAWD KA Sbjct: 241 TLICCRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWTSAPFEIPADVYSAWDAKA 300 Query: 301 QGQQLEADWNKLYQAYQAKYPTEAAELVRRLKGELPAGFDAAVQAYIASTIEKKETIATR 360 G EA W + + Y A+YP+EAAEL+RRLKGELPA F++ V AYI ST+EKKETIATR Sbjct: 301 TGADFEASWKQTFDTYAAQYPSEAAELLRRLKGELPAAFESTVTAYINSTLEKKETIATR 360 Query: 361 KASQNAIQAYAQVLPEFLGGSADLTGSNLTNWKESVAVRADVAGNHINYGVREFGMSAIM 420 KASQNAIQAYAQ LPEFLGGSADLTGSNLTNWKESVAVRAD GNHINYGVREFGMSAIM Sbjct: 361 KASQNAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIM 420 Query: 421 NGIALHGGYIPFGATFLTFSDYSRNALRMAALMKLRALFVFTHDSIGLGEDGPTHQSVEH 480 NGIALHGGY+PFGATFLTFSDYSRNALRMAALMK+RALFVFTHDSIGLGEDGPTHQSVEH Sbjct: 421 NGIALHGGYLPFGATFLTFSDYSRNALRMAALMKIRALFVFTHDSIGLGEDGPTHQSVEH 480 Query: 481 VSSLRLIPNLDNWRPCDTVESAVAWEQAVKRNDGPSTLIFSRQNLPFQERTPQQVADIKR 540 VSSLRLIPNLDNWRPCDT E+AVAW +++KR+DGPSTLIFSRQNLPFQERT QV DI R Sbjct: 481 VSSLRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDIAR 540 Query: 541 GGYILREAKDAKIILIATGSEIELAVKAADELAAQGIPARIVSMPSTDVFDRQDSAYKAS 600 GGY+LR+A +AK+IL+ATGSEIELA KAAD L A+GI R+VSMPSTDVFDRQD+AYKAS Sbjct: 541 GGYVLRDAPNAKVILMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDAAYKAS 600 Query: 601 VLQHGVPRVAIEAGVTSTWHKYVGLEGAVIGIDTFGESAPAGVLFKHFGFTVENVVAKAR 660 VL GVPRVAIEAGVT+ WHKYVGLEGAV+GIDTFGESAPAGVLFKHFGFTVENVVAKA+ Sbjct: 601 VLTRGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAK 660 Query: 661 LILA 664 +LA Sbjct: 661 AVLA 664 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 565 bits (1455), Expect = e-162, Method: Compositional matrix adjust. Identities = 295/661 (44%), Positives = 411/661 (62%), Gaps = 9/661 (1%) Query: 6 PTDKMANAIRALAMDAVQKANSGHPGAPMGMAEIAVALWAKHYRHNPTNPQWANRDRFVL 65 P +A+AIR L++DA+ +A GH G P+GMAEIA AL+A+H R NP P W +RDR VL Sbjct: 5 PLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVL 64 Query: 66 SNGHGSMLQYALLHLTGYD-LPMEEIKNFRQLHSKTAGHPEVHITPGVETTTGPLGQGIT 124 SNGHGSML Y LL+LTGY+ + +E++++FR+ S AGHPE G+E TTGPLGQGI Sbjct: 65 SNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLGQGIA 124 Query: 125 NAVGMALAEKLLAAEFNKPDLAVVDHYTYAFVGDGCLMEGISHEACSLAGTLRLSKLIVL 184 NA GMA+AE L A F +V+H TYAFVGDGCL EGI E SLAG LRL KL +L Sbjct: 125 NAFGMAVAEAYLNARFGDD---IVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLL 181 Query: 185 YDDNGISIDGKVEGWFKDDTPKRFEAYGWNVIPAVDGHNVEAVNSAIHQAKLADKPTLIC 244 +DDN I+ DGK E ++ +RF GW+V+ VDGH+++AV+ A+ A+ +D+P++I Sbjct: 182 WDDNQITDDGKTELSISENVAERFRVAGWHVV-EVDGHDIQAVSRALDAAQGSDRPSMIA 240 Query: 245 CKTVIGKGSPNLAGTDKVHGAALGDKEVAAVREALGWTSAPFEIPADIYSAWDGKAQGQQ 304 C+TVIG+G P + G H A L + + A RE L W APF++P DI + W ++ GQ+ Sbjct: 241 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQW--RSFGQR 298 Query: 305 LEADWNKLYQAYQAKYPTEAAELVRRLKGELPAGFDAAVQAYIASTIEKKETIATRKASQ 364 +A + QA + AE R ++GELP+G+ + + S E ++ S Sbjct: 299 NDAQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKRSAHEPEQAQGGILISA 358 Query: 365 NAIQAYAQVLPEFLGGSADLTGSNLTNWKESVAVRADVAGNHINYGVREFGMSAIMNGIA 424 + A+V+PE + G ADL D +G +++ GVRE M A+ NG+A Sbjct: 359 AINKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMA 418 Query: 425 LHGGYIPFGATFLTFSDYSRNALRMAALMKLRALFVFTHDSIGLGEDGPTHQSVEHVSSL 484 HGG I T+L FSDY R A+RM+ALM L +VF+HDSIG+G++GPTHQ VE ++SL Sbjct: 419 AHGGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASL 478 Query: 485 RLIPNLDNWRPCDTVESAVAWEQAVKRNDGPSTLIFSRQNLPFQERTPQQVADIKRGGYI 544 R +PN+ RP D E+ WE A++ GP +++F+RQ LP R + RGGY+ Sbjct: 479 RAMPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYV 538 Query: 545 LREAKDA--KIILIATGSEIELAVKAADELAAQGIPARIVSMPSTDVFDRQDSAYKASVL 602 L EA+ ++ L+ATGSE+ LA A +L A GI +VS+P ++FD+QD+AY+ VL Sbjct: 539 LHEAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVL 598 Query: 603 QHGVPRVAIEAGVTSTWHKYVGLEGAVIGIDTFGESAPAGVLFKHFGFTVENVVAKARLI 662 RV +EA + W Y+G G +G+ FG PA VL++HF T E VVA+AR + Sbjct: 599 GEQTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRL 658 Query: 663 L 663 L Sbjct: 659 L 659 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 119 bits (297), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 85/223 (38%), Positives = 115/223 (51%), Gaps = 17/223 (7%) Query: 38 EIAVALW-AKHYRHNPTNPQWANRDRFVLSNGHGSMLQYALLHLTGYDLPMEEIKNFRQL 96 +I VAL+ R NP +P+ A RDR V+S GHGS+ Y +L GY PMEE+++ + Sbjct: 39 DIFVALYYGGVLRFNPADPRDAGRDRCVISKGHGSICMYPILADLGY-FPMEELQHVCEA 97 Query: 97 HSKTAGHPEVHITPGVETTTGPLGQGITNAVGMALAEKLLAAEFNKPDLAVVDHYTYAFV 156 S G P+ + PG ET G LG G+ A GMAL K + D + Sbjct: 98 GSFLGGIPDP-VIPGYETVNGSLGHGLGVATGMALGLKRSGS----------DRSVFVVA 146 Query: 157 GDGCLMEGISHEACSLAGTLRLSKLIVLYDDNGISIDGKVEGWFKD-DTPKRFEAYGWNV 215 GDG L EG + EA A +L L ++ DDN IS+ G E + R A+GW+ Sbjct: 147 GDGELHEGANWEAIMFASQHKLDNLHLIVDDNRISMLGYTEDIVSHGELATRLSAFGWDC 206 Query: 216 IPAVDGHNVEAVNSAIHQAK--LADKPTLICCKTVIGKGSPNL 256 + VDGH+V AV +A+ Q K A KP I +T+ G G P L Sbjct: 207 M-EVDGHDVLAVQAALLQQKAHAAGKPKAIIARTLKGHGVPGL 248 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 88/257 (34%), Positives = 128/257 (49%), Gaps = 24/257 (9%) Query: 12 NAIRALAMDAVQKANSGHPGAPMGMAEIAVALWAKHYRHNPTNPQWANRDRFVLSNGHGS 71 NA+R M VQ G+ G +G A++ + P +PQW+ RDRF+LS+GH + Sbjct: 18 NAVR---MGEVQ--GQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSGRDRFLLSHGHYA 72 Query: 72 MLQYALLHLTGYDLPMEEIKNFRQLHSKTAGHPEVHITPGVETTTGPLGQGITNAVGMAL 131 + YA L G +P EE+ ++ S+ TPG+E + G LGQG+ VGMAL Sbjct: 73 IALYAALIEAGI-VPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLGQGLAIGVGMAL 131 Query: 132 AEKLLAAEFNKPDLAVVDHYTYAFVGDGCLMEGISHEACSLAGTLRLSKLIVLYDDNGIS 191 + N P + Y + DG L EG + EA A +L LI L D N Sbjct: 132 GLRY----NNNP------AFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINNQQ 181 Query: 192 ID---GKVEGWFKDDTPKRFEAYGWNVIPAVDGHNVEAVNSAIHQAK-LAD-KPTLICCK 246 D GK+ G+ + ++ A+GW + VDG+++ AV A A+ L D KP +I Sbjct: 182 ADGPSGKIMGF--EPLADKWAAFGW-YVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFD 238 Query: 247 TVIGKGSPNLAGTDKVH 263 T++GKG P L +K H Sbjct: 239 TLMGKGVPFLEQREKNH 255 >gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 321 Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 68/218 (31%), Positives = 107/218 (49%), Gaps = 29/218 (13%) Query: 398 VRADVAGNHINYGVREFGMSAIMNGIALHGGYIPFG---ATFLTFSDYSRNALRMAALMK 454 +RAD +N G+ E + + G+AL G Y F A F+T Y + + +A L + Sbjct: 41 IRADFPDRFVNVGIAEQNLINVSAGLALEG-YKVFAYAIAPFITMRCYEQVRVSLALLSE 99 Query: 455 LRALFVFTHDSIGLGE------DGPTHQSVEHVSSLRLIPNLDNWRPCDTVESAVAWEQA 508 +R + V + IG+G GPTHQ E ++ +R +PN D V +A A+ Sbjct: 100 VRPMNV---NLIGVGAGYSYVVSGPTHQCYEDITLMRALPNFQVLSAADHV-TAAAFFDT 155 Query: 509 VKRNDGPSTLIFSRQNLP--FQERTPQQVADIKRGGYILREAKDAKIILIATGSEIELAV 566 + GP L F Q LP + + P D+ +G +I R + KI L+ATG + A+ Sbjct: 156 CSNSMGPKYLRFDAQVLPVIYGDGAP----DLAQGFHIHR--RGGKIGLVATGYMLHTAL 209 Query: 567 KAADELAAQGIPARIVSMPSTDVFDRQDSAYKASVLQH 604 +AA+ LAA+GI ++ D+FD + + A LQ Sbjct: 210 QAAEVLAAEGIEVGVI-----DLFDL--ARFSADALQE 240 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,181,909 Number of extensions: 95978 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 5 Length of query: 664 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 573 Effective length of database: 1,188,985 Effective search space: 681288405 Effective search space used: 681288405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)