BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0145.p (716 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310752|ref|YP_003774844.1| glutamate-ammonia-ligase adenyl... 1001 0.0 >gi|300310752|ref|YP_003774844.1| glutamate-ammonia-ligase adenylyltransferase [Herbaspirillum seropedicae SmR1] Length = 927 Score = 1001 bits (2588), Expect = 0.0, Method: Compositional matrix adjust. Identities = 508/668 (76%), Positives = 570/668 (85%), Gaps = 11/668 (1%) Query: 1 VGTAAALNGPMRRLRNLIVCTLIERDLSGKADLAEVVRTMSEFADFAVQTHLQALMQEMT 60 + + +L MRRLRNL++CTLI RDL G+ DL EVV TM+ FADFAVQTHL ALMQE T Sbjct: 75 LASGLSLPAAMRRLRNLVICTLITRDLDGRGDLDEVVTTMTGFADFAVQTHLAALMQEQT 134 Query: 61 ALYGMPIGEESGSEQEMIVLGMGKLGGGELNVSSDIDLIFVYPEDGDTLAGQPGQRSLSN 120 ALYG+PIGEESG QEMIVLGMGKLGGGELNVSSDIDLIFVYPEDGDT A + GQ+SLSN Sbjct: 135 ALYGLPIGEESGRPQEMIVLGMGKLGGGELNVSSDIDLIFVYPEDGDTRA-EAGQKSLSN 193 Query: 121 HEFFIRLGKKLIAALSEITADGFTFRVDMALRPNGGSGPLVASHNMVEEYLIRQGREWER 180 HEFF+RLGKKLI AL+EIT DGFTFRVDMALRPNG SGPLVAS NMVEEYL+RQGREWER Sbjct: 194 HEFFVRLGKKLIGALAEITEDGFTFRVDMALRPNGNSGPLVASFNMVEEYLVRQGREWER 253 Query: 181 YAWTKARALTGKDSDIAALEAISRPFIYRRYLDFGSIDALRSMHAQIRAEVKRQESLHPE 240 YAWTKARALTG DIA LEAISRPFI+RRYLD+GSIDALRSMH QIRAEVKRQE+LHP+ Sbjct: 254 YAWTKARALTGTPEDIATLEAISRPFIFRRYLDYGSIDALRSMHGQIRAEVKRQEALHPD 313 Query: 241 RSNNVKLGRGGIREIEFTSQVFQLIRGGRDPELRDRSTRATLRTLADKNLLAPDVVAQLL 300 RSNNVKLGRGGIREIEFTSQVFQLIRGGRD ELRDRSTR TLRTLA K+LLAP+VV QLL Sbjct: 314 RSNNVKLGRGGIREIEFTSQVFQLIRGGRDAELRDRSTRTTLRTLAAKDLLAPEVVEQLL 373 Query: 301 EAYTFLRNLEHRLQYLEDAQTHTLPVNDSDQLIVAQMMGYADVAALLAELERHRRIVAGQ 360 AYTFLRNLEHRLQYLEDAQTHTLPVN D L+VA+MMGY + AALL ELER R IVA Q Sbjct: 374 HAYTFLRNLEHRLQYLEDAQTHTLPVNPDDLLLVARMMGYEEPAALLHELERQRAIVAAQ 433 Query: 361 FDTIFSDKQGGNNTDDRADSEGDGAVCELNDGESAESIADTLTLLGYDDVDGA--AQRVL 418 FD IF+DKQ ++++ DG ++D ++ E +AD L L+G+ + D A A+R+ Sbjct: 434 FDAIFADKQ--------SEADNDGPSVSVSDNDNLEGLADALRLVGFPEADIADGARRLH 485 Query: 419 ATLQSPRLQSLPEASRTRLMTLINAALPIIGAMPDKRLPTLRRLLDFFEAIARRAAYLAL 478 T QSPR+QSLPEASR RL T+IN +LP++ A+ +LP L RLLD EAIARRAAYLAL Sbjct: 486 LTWQSPRMQSLPEASRNRLNTVINTSLPLLAALHYDQLPALGRLLDLLEAIARRAAYLAL 545 Query: 479 LTEYPHALTRVIRMMAASDWAAQYLTQHPILLDELLDDRNLKAPPDWPAFTQDCQRQLDT 538 LTEYP+AL R++RM+ AS WAA YLT+HP+LLDELLDDRNLKA DW AF ++C+RQL T Sbjct: 546 LTEYPYALQRLVRMIGASGWAATYLTRHPLLLDELLDDRNLKAASDWAAFAENCRRQLAT 605 Query: 539 APGDTERQMDILREVHHAQLFRLLAQDLEGELSVEHLADHLSALADILVAATIQAVWQTI 598 A GDTERQ+DILRE+HHA+ FRLLAQDLEG+LSVE LAD LSALAD+LV TI+AVWQT+ Sbjct: 606 AEGDTERQLDILRELHHAEQFRLLAQDLEGDLSVEKLADELSALADVLVQVTIEAVWQTV 665 Query: 599 ANRHREVPQFTVIAYGKLGGKELGYVSDLDVVFLYDDDDQEAPGLYAKLAQRFITWMTSH 658 A RHREVPQF VIAYGKLGGKELGY SDLDVVFLYDD+DQ+APGLYAKLAQRFITWMTSH Sbjct: 666 AQRHREVPQFAVIAYGKLGGKELGYASDLDVVFLYDDEDQDAPGLYAKLAQRFITWMTSH 725 Query: 659 TPAGTLFD 666 TPAGTLFD Sbjct: 726 TPAGTLFD 733 Score = 101 bits (252), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 90/330 (27%), Positives = 147/330 (44%), Gaps = 33/330 (10%) Query: 11 MRRLRNLIVCTLIERDLSGKADLAEVVRTMSEFADFAVQTHLQALMQEMTALYGMPIGEE 70 +R L + L+ +DL G + ++ +S AD VQ ++A+ Q + + Sbjct: 617 LRELHHAEQFRLLAQDLEGDLSVEKLADELSALADVLVQVTIEAVWQ--------TVAQR 668 Query: 71 SGSEQEMIVLGMGKLGGGELNVSSDIDLIFVYP-EDGDTLAGQPGQRSLSNHEFFIRLGK 129 + V+ GKLGG EL +SD+D++F+Y ED D PG + +L + Sbjct: 669 HREVPQFAVIAYGKLGGKELGYASDLDVVFLYDDEDQDA----PG--------LYAKLAQ 716 Query: 130 KLIAALSEITADGFTFRVDMALRPNGGSGPLVASHNMVEEYLIRQGREWERYAWTKARAL 189 + I ++ T G F +D+ALRP+G SG LV+ + E+Y + WE A T+AR Sbjct: 717 RFITWMTSHTPAGTLFDIDIALRPDGASGLLVSPLSSFEKYQLNAAWIWEHQALTRARFC 776 Query: 190 TGKDSDIAALEAISRPFIYRRYLDFGSIDALRSMHAQIRAEVKRQESLHPERSN--NVKL 247 G + EA+ R + R+ D ++ ++ + +R HP RS ++K Sbjct: 777 AGDTAIGERFEAL-RERVLRQPRDPARLE------EEVLSMRRRMREAHPNRSTMFDLKH 829 Query: 248 GRGGIREIEFTSQVFQLIRGGRDPELR-DRSTRATLRTLADKNLLAPDVVAQLLEAYTFL 306 GG+ +IEF Q L P+L D A L+ L+ + + AY Sbjct: 830 DEGGMIDIEFMVQYLVLRHASDHPQLTGDIGNIALLKLAGQLGLIDALLADEAANAYRLF 889 Query: 307 RNLEH--RLQYLEDAQTHTLPVNDSDQLIV 334 R L+H RLQ E A + V ++ Sbjct: 890 RKLQHQIRLQGSERAHIDAIRVEHERACVI 919 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,208,671 Number of extensions: 93262 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 2 Length of query: 716 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 624 Effective length of database: 1,184,250 Effective search space: 738972000 Effective search space used: 738972000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)