BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0144.p (281 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310753|ref|YP_003774845.1| transmembrane protein [Herbaspi... 311 4e-86 >gi|300310753|ref|YP_003774845.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1439 Score = 311 bits (796), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 146/252 (57%), Positives = 194/252 (76%), Gaps = 1/252 (0%) Query: 1 MALKATPPADYAAPLDVRANFYHPSFASKISDVTRWKGTLYADVSNTDLAVWKAYVDYPL 60 ++L+A PP YAAPLDVRA+F HP+FA + SD+ RWKGTLYAD+ TDL+VW+AY DYP+ Sbjct: 220 LSLRAIPPTSYAAPLDVRADFSHPAFA-RSSDILRWKGTLYADLQRTDLSVWRAYFDYPI 278 Query: 61 ELNQGRGSVRAWLDLDHNKIANFTADLSLTNLSARLDKKLEPLQLARVSGRVSGSEIYAP 120 + G GSVRAWL LDH K+ANFTADL+L N +A+L ++LEPL L RV+GR+S SE Sbjct: 279 AIQSGTGSVRAWLALDHAKVANFTADLALNNFNAQLSRQLEPLSLKRVNGRISASESLGA 338 Query: 121 DLQDGIPTFGANGHKLTLSNFSLETDDGFALPPTTIFEVFEPATRFRPEKMTVEAQLLDL 180 +DGIPTFGANGH++TL++FS+ET DGF LPPT+I E +EP T + E+ +V+A L+L Sbjct: 339 TPEDGIPTFGANGHQVTLTDFSIETPDGFVLPPTSIAESYEPGTPLKAERTSVKATYLNL 398 Query: 181 AILSDLGSRLPLTPSQHKLLADLAPHGQLKDFSVQWEGSYPDVRAYRLRGAFSGLGMQPQ 240 LS L +RLPL PSQ KLL DLAP G+L+DF+VQW+G+YP++ +YR++G FSGLG++ Sbjct: 399 QTLSQLATRLPLAPSQRKLLDDLAPRGELRDFTVQWQGTYPELASYRVQGRFSGLGLKGL 458 Query: 241 AARLAQPKTATQ 252 A + + A Q Sbjct: 459 PAHVEPRRNAAQ 470 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 966,439 Number of extensions: 41559 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 1 Length of query: 281 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 197 Effective length of database: 1,222,130 Effective search space: 240759610 Effective search space used: 240759610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)