BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0140.p (486 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 878 0.0 gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 503 e-143 gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 77 4e-15 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 73 6e-14 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 878 bits (2268), Expect = 0.0, Method: Compositional matrix adjust. Identities = 444/486 (91%), Positives = 471/486 (96%) Query: 1 MKTFEPNLSALGIARDVLLTPFGLDEAKLLKTLGTMFTHKVDYADLYFQFTKSEGWSLEE 60 MK FEPN+ AL +ARDVLLTPFGLDE+KLLK LG+MFTHKVDYADLYFQFTKSEGWSLEE Sbjct: 1 MKLFEPNMGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEE 60 Query: 61 GIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISESILLDAAAATRTIGRQGAGRVKIAGS 120 GIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISES L+DAAAATRTI RQG+G++K+AG+ Sbjct: 61 GIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGA 120 Query: 121 MQPNGGRSLYLPHDPLTSLDATAKVNLLERIERIARAKDPRVVQVMASLAGEYDVVLVAR 180 M P GGR+LYLPHDPLTSLDATAKV LLER+ERIARAKDPRV QVMASL+GEYDVVLVAR Sbjct: 121 MIPGGGRALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVAR 180 Query: 181 SDGIIAADIRPLVRVSITVIAEQNGRREMGSSGGGGRYSYAYFSDELLEKYASEAVSTAL 240 +DG++AADIRPLVRVS+TVIAEQNGRREMGSSGGGGRY Y YFSD LLE+YASEAV+TAL Sbjct: 181 ADGVLAADIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATAL 240 Query: 241 VNLDARPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVT 300 VNL++RPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVT Sbjct: 241 VNLESRPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVT 300 Query: 301 VVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMPVTGNARRESF 360 VVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKM VTGNARRESF Sbjct: 301 VVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESF 360 Query: 361 AHLPMPRMTNTYMLAGDKDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEN 420 AHLPMPRMTNTYML GD+DPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIE+ Sbjct: 361 AHLPMPRMTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIED 420 Query: 421 GKISYPVKGATLIGNGPDVLNRVSLIGNDMKLDSGVGVCGKEGQSVPVGVGQPTLRIDGV 480 GKI+YPVKGATLIGNGPDVLNRVS+IGNDM+LDSGVGVCGKEGQSVPVGVGQPTLR+DGV Sbjct: 421 GKITYPVKGATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGV 480 Query: 481 TVGGTA 486 TVGGTA Sbjct: 481 TVGGTA 486 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 503 bits (1295), Expect = e-143, Method: Compositional matrix adjust. Identities = 261/474 (55%), Positives = 333/474 (70%), Gaps = 2/474 (0%) Query: 14 ARDVLLTPFGLDEAKLLKTLGTMFTHKVDYADLYFQFTKSEGWSLEEGIVKTGSFSIDQG 73 AR LL GLDE++LL + DYADLY + + +E W LE G V +++ QG Sbjct: 29 ARWGLLAASGLDESQLLAGVSRALRKGGDYADLYLKSSVAESWHLERGKVTRSNYNQSQG 88 Query: 74 VGVRAVSGDKTAFSYSDEISESILLDAA-AATRTIGRQGAGRVKIAGSMQPNGGRSLYLP 132 G+R V G+++ + +D + + AA A +G + + P G + Y Sbjct: 89 FGLRLVEGEQSVLTSADRFDATTIGKAADLAADAVGHAPVSPAGLVLKL-PKRGPTYYTL 147 Query: 133 HDPLTSLDATAKVNLLERIERIARAKDPRVVQVMASLAGEYDVVLVARSDGIIAADIRPL 192 DPL SLDA K+ LLE ++R RA+ P VV+VMASL+ D+V +AR+DG D+RPL Sbjct: 148 DDPLPSLDAAHKIALLESVDRACRARSPLVVEVMASLSISLDLVFLARNDGYCGGDVRPL 207 Query: 193 VRVSITVIAEQNGRREMGSSGGGGRYSYAYFSDELLEKYASEAVSTALVNLDARPAPAGP 252 VR+ + V +NGRRE S GGR A F +E A+ AV AL+ L+AR APAG Sbjct: 208 VRLDVAVQVVRNGRREGASRTLGGRVGLATFDHAAIESVAALAVDAALIKLEARAAPAGT 267 Query: 253 MTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRG 312 MTVV+GPGW G++LHEA+GHGLEGDFNR+GSS F+GRIG+RVAA GVT+VDDGT+ RG Sbjct: 268 MTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSAFAGRIGQRVAAPGVTIVDDGTVPGSRG 327 Query: 313 SLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMPVTGNARRESFAHLPMPRMTNTY 372 SLNIDDEG P QCTTLIEDGIL Y+QD++NARLM + TGN RRES+ LPMPRMTNTY Sbjct: 328 SLNIDDEGTPGQCTTLIEDGILVSYMQDSLNARLMGVANTGNGRRESYDVLPMPRMTNTY 387 Query: 373 MLAGDKDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIENGKISYPVKGATL 432 M AG DPAEI+ASVK G+Y + GGQVDI++G+F+F+ASEA++IENG++ PVKGAT+ Sbjct: 388 MRAGGVDPAEIIASVKQGIYIAHLEGGQVDISSGRFMFAASEAFLIENGRLGAPVKGATV 447 Query: 433 IGNGPDVLNRVSLIGNDMKLDSGVGVCGKEGQSVPVGVGQPTLRIDGVTVGGTA 486 +GNGP L V LIGND+++++ GVCGK GQSVPVGVGQPTLRIDG+TVGGTA Sbjct: 448 LGNGPQSLKNVKLIGNDLEINTASGVCGKAGQSVPVGVGQPTLRIDGMTVGGTA 501 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 76.6 bits (187), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 84/319 (26%), Positives = 134/319 (42%), Gaps = 24/319 (7%) Query: 138 SLDATAKVNLLERIERIARAKDPRVVQVM-ASLAGEYDVVLVARSDGIIAADIRPLVRVS 196 ++ A V L +R E A DPR+ A + ++ + A S G + P R + Sbjct: 131 NISAEEAVALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGFMGG--YPFSRHT 188 Query: 197 ITV--IAEQNGRREMGSSGGGGRYSYAYFSDELLEKYASEAVSTALVNLDARPAPAGPMT 254 I+V IA + + R E + +YA+E AL L+AR Sbjct: 189 ISVAPIAGKGASMQRDDWYSSKRDPKKLAQPEAIGRYAAE---RALARLNARKLSTRKCP 245 Query: 255 VV----LGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADR 310 V+ L G G + G G RK S+ +G++V + + +++D + Sbjct: 246 VLFEAPLAAGLLGAFVQAVSG----GALYRK-STFLLDSLGQQVFPEHIQILEDPHVIGG 300 Query: 311 RGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMPVTGNARRESFAHLPMPRMTN 370 GS D+EG T ++ +G+++GY T +AR + M TGN+ L T Sbjct: 301 VGSAPFDEEGVRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSELTTR 360 Query: 371 TYMLAGDKDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIENGKISYPVKGA 430 G +L + GL G V+ G + AS Y +ENG I YPV+ Sbjct: 361 ADTFKG------MLKKMGTGLLVTELMGQGVNYVTGDYSRGAS-GYWVENGVIQYPVEEI 413 Query: 431 TLIGNGPDVLNRVSLIGND 449 T+ GN D+L ++ +GND Sbjct: 414 TIAGNMKDMLRQIVAVGND 432 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 82/312 (26%), Positives = 129/312 (41%), Gaps = 12/312 (3%) Query: 145 VNLLERIERIARAKDPRVVQV-MASLAGEYDVVLVARSDGIIAADIRPLVRVSITVIAEQ 203 V L+ RIER A A V Q+ A L ++A S+G + + +S T +A + Sbjct: 141 VALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEGFLRSSASTRHSLSCTAVAIR 200 Query: 204 NGRREMGSSGGGGRYSYAYFSDELLEKYASEAVSTALVNLDARPAPAGPMTVVLGPGWPG 263 +G +E+ S R S E L A AL L AR V+ P Sbjct: 201 DGAKEIESWWDARRDPAQLPSPESLGACAGR---RALARLGARQVATQTCPVLFEPQAAA 257 Query: 264 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 323 LL+E + + SS +G+ V A + + DD + GS + D G Sbjct: 258 SLLNEFV-QAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGNGAAL 316 Query: 324 QCTTLIEDGILKGYIQDTMNARLMKMPVTGNARRESFAHLPMPRMTNTYMLAGDKDPAEI 383 + TLI G L+GY AR + +P+ + P M ++ A D + Sbjct: 317 RPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAG------PSNVMVSSTTSAASGDLEAM 370 Query: 384 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIENGKISYPVKGATLIGNGPDVLNRV 443 + + GL G V++ G F + S + +ENG+I+YPV G T+ N ++ + Sbjct: 371 IRRMGTGLVISGLNGQGVNLMTGDFSRACS-GFWVENGQIAYPVAGITVASNLREMFAGI 429 Query: 444 SLIGNDMKLDSG 455 +GND +G Sbjct: 430 VAVGNDRLTQNG 441 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,516,851 Number of extensions: 67067 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 4 Length of query: 486 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 397 Effective length of database: 1,198,455 Effective search space: 475786635 Effective search space used: 475786635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)