BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0127.p (222 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 338 2e-94 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 199 2e-52 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 186 1e-48 gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid... 184 7e-48 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 156 1e-39 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 338 bits (866), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 169/221 (76%), Positives = 191/221 (86%), Gaps = 1/221 (0%) Query: 1 VTNTPDVLTDDVADFAMTLLLSTARRVVDADRFARSGEWKKGPFALTTKVTGSRLGIVGL 60 VT+TPDVL DDVAD AM L+L+TAR VV ADRFARSGEWKKGPF TTKV+G+RLGIVGL Sbjct: 96 VTHTPDVLNDDVADLAMALMLATARNVVRADRFARSGEWKKGPFPFTTKVSGARLGIVGL 155 Query: 61 GRIGKAIARRAAAFDMEISYHNRSPQSDVPYRYVADLKTLAGEVDFLVLSMPGGARARAL 120 GRIG+AIA+RAAAFDM+ISYHNRS + DVPY YV + LA EVDFLV+ PGGA RAL Sbjct: 156 GRIGQAIAKRAAAFDMQISYHNRS-RKDVPYTYVDSITALAREVDFLVMITPGGAGTRAL 214 Query: 121 INAEVLDALGPKGFLINVARGSVVDEAALIKALQEGRIAGAGLDVFENEPNVPEALAKLP 180 +NAEVL+ALGPKGFLINVARGSVVDEAALI AL+ G IAGAGLDVF +EPNVP LA L Sbjct: 215 VNAEVLEALGPKGFLINVARGSVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALD 274 Query: 181 NLTLTPHMASGTVITRTAMADLAFDNLQAHFSGKPLLTPVP 221 N+ LTPHMASGT++TRTAMADLAF+NLQAHFSG +++PVP Sbjct: 275 NVVLTPHMASGTLVTRTAMADLAFNNLQAHFSGAGVISPVP 315 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 199 bits (505), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 104/209 (49%), Positives = 136/209 (65%), Gaps = 2/209 (0%) Query: 10 DDVADFAMTLLLSTARRVVDADRFARSGEWKKGPFALTTKVTGSRLGIVGLGRIGKAIAR 69 D VAD A+ LLL+ R + DR+ R G W++ L ++ G R+GIVG+G IGK IAR Sbjct: 101 DCVADHALGLLLAILRNIPVLDRYTRDGGWRE-TIPLQPQLAGKRVGIVGMGNIGKKIAR 159 Query: 70 RAAAFDMEISYHNRSPQSDVPYRYVADLKTLAGEVDFLVLSMPGGARARALINAEVLDAL 129 RAAAFD EI+Y NR + DV Y Y D+ LAG D L+++ PGGA+ R LINA VL+ L Sbjct: 160 RAAAFDAEIAYCNRKKRDDVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEEL 219 Query: 130 GPKGFLINVARGSVVDEAALIKALQEGRIAGAGLDVFENEPNVPEALAKLPNLTLTPHMA 189 GP+G+L+N+ RGS+VD AL AL GR+AGAGLDV+E EP P AL LPN+ LTPH+A Sbjct: 220 GPQGYLVNIGRGSIVDTDALGAALSSGRLAGAGLDVYEGEPQPPAALIALPNVVLTPHIA 279 Query: 190 SGTVITRTAMADLAFDNLQAHF-SGKPLL 217 + A N + HF + +P+L Sbjct: 280 GWSPEAIRASVTQFLRNCEEHFAAAEPVL 308 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 186 bits (473), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 101/221 (45%), Positives = 140/221 (63%), Gaps = 9/221 (4%) Query: 2 TNTPDVLTDDVADFAMTLLLSTARRVVDADRFARSGEWKKGPFA--LTTKVTGSRLGIVG 59 TNTPDVL + ADF L+++ ARR+ +++ F R+G+WKK + + + G+ LGI+G Sbjct: 94 TNTPDVLNETTADFGWALMMAAARRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIG 153 Query: 60 LGRIGKAIARRAAAFDMEISYHNRS---PQSDV---PYRYVADLKTLAGEVDFLVLSMPG 113 +GRIG+AIARR+ FDM++ YHNRS P+ + RYV+ + L + D ++L +P Sbjct: 154 MGRIGQAIARRSLGFDMQVLYHNRSRLAPELEARANHARYVSK-EELLRQADHVILVLPY 212 Query: 114 GARARALINAEVLDALGPKGFLINVARGSVVDEAALIKALQEGRIAGAGLDVFENEPNVP 173 I A L + P L N+ARG +VD+AALI AL+EGRIA AG+DVFENEP Sbjct: 213 SKDTHHTIAAAELALMKPTATLTNIARGGIVDDAALIAALREGRIAAAGVDVFENEPAFK 272 Query: 174 EALAKLPNLTLTPHMASGTVITRTAMADLAFDNLQAHFSGK 214 L N+ LTPH+AS + TR AMA+ A DNL A SG+ Sbjct: 273 PEFLDLSNVVLTPHIASASTPTRLAMANCAADNLIAALSGQ 313 >gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 348 Score = 184 bits (466), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 99/218 (45%), Positives = 142/218 (65%), Gaps = 5/218 (2%) Query: 1 VTNTPDVLTDDVADFAMTLLLSTARRVVDADRFARSGEWKKGP----FALTTKVTGSRLG 56 VT T LT+DVAD A+ L+L+ +R++ F + G+W + P L+ +++G R+G Sbjct: 124 VTGTFGALTEDVADLALGLMLAVSRQICPGHAFVQRGDWVRHPSPTAIPLSRRLSGKRVG 183 Query: 57 IVGLGRIGKAIARRAAAFDMEISYHNRSPQSDVPYRYVADLKTLAGEVDFLVLSMPGGAR 116 IVG+G++G+AIA+RAAAF I+Y + D+PY + +L LA E D LVL+ + Sbjct: 184 IVGMGKVGRAIAQRAAAFGCPITYTDLRAMEDLPYAFQPELLQLARESDVLVLA-AAADK 242 Query: 117 ARALINAEVLDALGPKGFLINVARGSVVDEAALIKALQEGRIAGAGLDVFENEPNVPEAL 176 A+ +INA VLDALG +G LIN+ARG +V E L+ ALQ G IAGAGLDVF +EPNVP AL Sbjct: 243 AQGIINAAVLDALGREGILINIARGKLVVEEDLVDALQRGVIAGAGLDVFVDEPNVPAAL 302 Query: 177 AKLPNLTLTPHMASGTVITRTAMADLAFDNLQAHFSGK 214 ++ + L H AS TV +RTAM ++ +L +G+ Sbjct: 303 LQMDQVVLQAHRASATVESRTAMGEMVLASLAQGLAGQ 340 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 156 bits (395), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 95/224 (42%), Positives = 129/224 (57%), Gaps = 9/224 (4%) Query: 1 VTNTPDVLTDDVADFAMTLLLSTARRVVDADRFARSGEWKK--GPFALTTKVTGSRLGIV 58 + +TP VLT+ AD L+L++ARRVV+ + ++G WK G V G LG++ Sbjct: 92 LAHTPGVLTEATADTIFALILASARRVVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLI 151 Query: 59 GLGRIGKAIARRAA-AFDMEISYHNRSP----QSDVPYRYVADLKTLAGEVDFLVLSMPG 113 G+GRIG A+ARRA F M I YHNR P + ++ RYV+ LA + DF+ + +P Sbjct: 152 GMGRIGSAVARRAHHGFGMPILYHNRRPDPEAERELGARYVSKEDLLA-QADFVCVMLPL 210 Query: 114 GARARALINAEVLDALGPKGFLINVARGSVVDEAALIKALQEGRIAGAGLDVFENEP-NV 172 + + A + IN +RG +VDEAALI ALQ+ I GAGLDVFE EP + Sbjct: 211 TPQTERMFGAPEFALMKRSAIFINASRGRIVDEAALIAALQDKTIHGAGLDVFEVEPLPL 270 Query: 173 PEALAKLPNLTLTPHMASGTVITRTAMADLAFDNLQAHFSGKPL 216 L KLPN+ PH+ S T TR AMA+LA NL A G+P+ Sbjct: 271 QSPLLKLPNVVALPHIGSATHETRLAMAELAVTNLIAGLRGEPV 314 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 626,434 Number of extensions: 23342 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 11 Length of query: 222 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 140 Effective length of database: 1,231,600 Effective search space: 172424000 Effective search space used: 172424000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)