BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0124.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 290 1e-79 gi|300310763|ref|YP_003774855.1| ATP-binding protein [Herbaspiri... 172 4e-44 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 103 2e-23 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 93 4e-20 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 92 1e-19 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 290 bits (742), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 134/152 (88%), Positives = 142/152 (93%), Gaps = 2/152 (1%) Query: 228 PQVMAGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDMILVSGFNVYPNEI 287 PQVM GYW RPDET KVMTPDGFFK+GDIG+MDERGYT+IVDRKKDMILVSGFNVYPNE+ Sbjct: 415 PQVMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEV 474 Query: 288 EGVVAAHPGVLECACVGVPDEHTGEAVKLFVVRKDKSLTAEQLMAYCKEQFTGYKKPKYI 347 EGVVA HPGVLECACVGVPDEHTGEAVKLFVVRKDK+L+A QLM YCKEQFTGYKKPKYI Sbjct: 475 EGVVAQHPGVLECACVGVPDEHTGEAVKLFVVRKDKTLSAAQLMDYCKEQFTGYKKPKYI 534 Query: 348 EFRDELPKTNVGKILRRELRDE--KKTEKKAA 377 EFRDELPKTNVGKILRRELRDE K +K+AA Sbjct: 535 EFRDELPKTNVGKILRRELRDEPAKAQQKQAA 566 Score = 165 bits (417), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 76/104 (73%), Positives = 91/104 (87%) Query: 119 LKDSGAEAIFVLENFACTLAKVIANTDVRHVVVASMGEMLGGLKGAIANLVVRHVKKLVP 178 LKDSGAEAIFVLENFACTL KV+A+T+++H+VVASMGE+LG LKG + NLVVRHVKKLVP Sbjct: 116 LKDSGAEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVP 175 Query: 179 PFSLPQSITFKQALSRGHGRTPAPIEVGHDDVAFLQYTGGTTGV 222 +SLP +ITF QALS+ G+T P+ + HDD+AFLQYTGGTTGV Sbjct: 176 AWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGV 219 >gi|300310763|ref|YP_003774855.1| ATP-binding protein [Herbaspirillum seropedicae SmR1] Length = 311 Score = 172 bits (436), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 83/104 (79%), Positives = 91/104 (87%) Query: 3 ASKNTTEIGTIIAAHGRHYLAQVGEEKLQCVTRGKKSDVAVGDKVNLKRTSANQAVIESI 62 A+ + E G +IAAHGRHYL Q GEEKL CVTRGKKSDVAVGD++ L RTSA+Q VIESI Sbjct: 13 ATPDALEPGLVIAAHGRHYLVQAGEEKLHCVTRGKKSDVAVGDRIRLARTSADQGVIESI 72 Query: 63 DERKTLLYRSDQYKSKLLASNVTQLFIVVATEPGFTDDLISRAL 106 DERKTLLYRSDQYKSKLLA+NV QLFIVVATEP FTDDL+SRAL Sbjct: 73 DERKTLLYRSDQYKSKLLAANVDQLFIVVATEPSFTDDLVSRAL 116 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 64/196 (32%), Positives = 101/196 (51%), Gaps = 8/196 (4%) Query: 176 LVPPFSLPQSITFKQAL--SRGHGRTPAPIEVGHDDVAFLQ-YTGGTTGVRFFFXPQVMA 232 +PP+ + QA + G RT I + +DD L + G VR P V Sbjct: 354 FLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFETGEICVR---GPAVFM 410 Query: 233 GYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDMILVSGFNVYPNEIEGVVA 292 GY N P+ K D +F +GD+G +D+ G+ I R DM + G NVYP EIE + Sbjct: 411 GYHNNPEANAKAFKHD-WFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALL 469 Query: 293 AHPGVLECACVGVPDEHTGEA-VKLFVVRKDKSLTAEQLMAYCKEQFTGYKKPKYIEFRD 351 HP V E A +GVPD GE+ + + V + + + E L+A+ + + YK P+ + D Sbjct: 470 THPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLAHLESRIGKYKWPRRFIYWD 529 Query: 352 ELPKTNVGKILRRELR 367 +PK+ GKI++++++ Sbjct: 530 SMPKSGYGKIVKKQIK 545 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 92.8 bits (229), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 54/146 (36%), Positives = 77/146 (52%), Gaps = 7/146 (4%) Query: 228 PQVMAGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRIVDRKKDMILVSGFNVYPNEI 287 P + YW + T + D F +GD+G MDE+GY R + R D+I +G+ + P I Sbjct: 403 PVMFLRYWRNEEATREKFAGD-FLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAI 461 Query: 288 EGVVAAHPGVLECACVGVPDEHTGEAVKLFVVRK-----DKSLTAEQLMAYCKEQFTGYK 342 E + HP V A VGV D E VK FVV K D++L AE L + + Q ++ Sbjct: 462 EECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKAE-LQQHVRAQLAAHE 520 Query: 343 KPKYIEFRDELPKTNVGKILRRELRD 368 P+ I F LP T GKI+R+ L++ Sbjct: 521 YPRLISFVAALPTTATGKIMRKTLKE 546 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 91.7 bits (226), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 61/168 (36%), Positives = 87/168 (51%), Gaps = 3/168 (1%) Query: 208 DDVAFLQYTGGTTGVRFFFXPQVMAGYWNRPDETEKVMTPDGFFKSGDIGIMDERGYTRI 267 D +A + G G F M GY T + G+F +GD+G+M+ GY +I Sbjct: 377 DTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAFA-GGWFHTGDLGVMNADGYIKI 435 Query: 268 VDRKKDMILVSGFNVYPNEIEGVVAAHPGVLECACVGVPDEHTGEAVKLFV-VRKDKSLT 326 DR KD+I+ G N+ E+E V+ HP VL A V PDE GE FV +++ + Sbjct: 436 KDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVG 495 Query: 327 AEQLMAYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELRDEKKTEK 374 A +L +C+ G+K PK I F LPKT+ GKI + ELR K++ Sbjct: 496 AAELTEFCRSHLAGFKVPKAIYF-GPLPKTSTGKIQKFELRKRMKSDS 542 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,070,979 Number of extensions: 41421 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 11 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)