BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0095.p (529 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspiri... 962 0.0 gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herb... 284 1e-77 >gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspirillum seropedicae SmR1] Length = 511 Score = 962 bits (2487), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/511 (89%), Positives = 483/511 (94%) Query: 19 MKPISPVIRLNPVDDVVIARRQLITGTILEEENGLRVAALIPAGHKMATRAIAVGQPIKR 78 MK SPVIRLNPVDDVVIAR+QLI+GT+L++E GL+V LIPAGHKMATRAI G+P+KR Sbjct: 1 MKAHSPVIRLNPVDDVVIARQQLISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPVKR 60 Query: 79 YGQIIGTASQAIEPGQHVHTHNLAMAEFSREHSFGADFKPVDYVDTPATFLGIKRQDGRV 138 YGQIIGTASQ I PGQHVHTHNLAMAEFSREH FGAD KPVD+V TPATF+GI R DGRV Sbjct: 61 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPATFMGIVRPDGRV 120 Query: 139 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEALADFPNVDGIVALTHGLGCAVDSEGE 198 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPE LAD+PN+DG+VALTHG GCA+DS+GE Sbjct: 121 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHGQGCALDSQGE 180 Query: 199 PLAMLRRTLAGYAKHPNFAAVLIVGLGCETNQISGLLEAHNLKENEYFHTFTIQSTGGTA 258 PL +LRRTLAGYA HPNFAAVL+VGLGCETNQISGL+E+HNLKE EYFHTFTIQ TGGTA Sbjct: 181 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA 240 Query: 259 KTVALGIEKIKKMLPKANDVKREPVPASHLTLGLQCGGSDGYSGITANPALGAAVDLLVR 318 KTVALGIEKIKKMLPKAND+KREPV A HLTLGLQCGGSDGYSGITANPALGAAVDLLVR Sbjct: 241 KTVALGIEKIKKMLPKANDIKREPVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR 300 Query: 319 HGGTAILSETPEIYGAEHLLTRRAVSSEVGEKLLARIAWWEEYCAKNGAEMNNNPSAGNK 378 HGGTAILSETPEIYGAEHLLTRRAVS EVGEKLLARIAWWEEYCAKN AEMNNNPSAGNK Sbjct: 301 HGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWWEEYCAKNDAEMNNNPSAGNK 360 Query: 379 AGGLTTILEKSLGAVAKGGTSNLMDVYKYAETVTAKGFVFMDTPGYDPISATGQVAGGAN 438 AGGLTTILEKSLGAVAKGGT+NL+DVYKYAE VTA+GFVFMDTPGYDPISATGQVAGGAN Sbjct: 361 AGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVFMDTPGYDPISATGQVAGGAN 420 Query: 439 MICFTTGRGSAYGCAPSPSLKLATNTALWQRQEEDMDINCGEIADGLATPQEIGAKFFQM 498 MICFTTGRGSAYGCAP+PSLKLATNTALWQRQEEDMDINCGEIADG TPQEIG +FFQM Sbjct: 421 MICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGNVTPQEIGERFFQM 480 Query: 499 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT 529 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT Sbjct: 481 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT 511 >gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 284 bits (727), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 177/497 (35%), Positives = 263/497 (52%), Gaps = 27/497 (5%) Query: 26 IRLNPVDDVVIARRQ--LITGTILEEENGLRVAALIPAGHKMATRAIAVGQPIKRYGQII 83 I +N D+V I L GT+ + GL + +P GHK+A R + G+ I RY I Sbjct: 16 IMMNDTDNVAIVVNDGGLPAGTVFPD--GLTLVDRVPQGHKIALRDLKQGEAIVRYDVAI 73 Query: 84 GTASQAIEPGQHVHTHNLAMAEFSREHSFGADFKPVDYVDTPA--------TFLGIKRQD 135 G A + I G + +L +RE D P+ PA TF G + D Sbjct: 74 GYAVRDIPKGGWIE-ESLVQMPPARE----LDNLPI-ATKKPAPQPPLEGYTFEGYRNAD 127 Query: 136 GRVATRNYIGVLTSVNCSATAARAIADYFRRDIHPEALADFPNVDGIVALTHGLGCAVDS 195 G V TRN + + T+V C A + ++ + I E L +PNV+ +VAL H GC V Sbjct: 128 GSVGTRNLLAITTTVQCVA----GVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCGVAI 183 Query: 196 EGEPLAMLRRTLAGYAKHPNFAA-VLIVGLGCETNQISGLLEAHNLKENEYFHTFTIQST 254 + + RTL + +PNF ++V LGCE Q + LL + + ++ + + Sbjct: 184 DAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKQGEPYVVCLQ 243 Query: 255 GGTAKTVALGIEKIKKM----LPKANDVKREPVPASHLTLGLQCGGSDGYSGITANPALG 310 I+ I M L + N +RE PAS L +G+QCGGSD +SG+TANPA+G Sbjct: 244 DAEHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVTANPAVG 303 Query: 311 AAVDLLVRHGGTAILSETPEIYGAEHLLTRRAVSSEVGEKLLARIAWWEEYCAKNGAEMN 370 A DLLVR G + + SE E+ LT RAV+ EV + ++ + W++ Y + G + + Sbjct: 304 FATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQGGVDRS 363 Query: 371 NNPSAGNKAGGLTTILEKSLGAVAKGGTSNLMDVYKYAETVTAKGFVFMDTPGYDPISAT 430 N + GNK GGL I+EK++G++ K G+S + V + + KG ++ TP D I T Sbjct: 364 ANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLSPGDKLQQKGLIYAATPASDFICGT 423 Query: 431 GQVAGGANMICFTTGRGSAYGCAPSPSLKLATNTALWQRQEEDMDINCGEIADGLATPQE 490 Q+A G N+ FTTGRG+ YG A P +K+AT L +R + MD+N G IA G A+ ++ Sbjct: 424 LQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEASIED 483 Query: 491 IGAKFFQMILDTASGKK 507 +G + FQ++LD ASGKK Sbjct: 484 VGWELFQLMLDVASGKK 500 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,790,295 Number of extensions: 80587 Number of successful extensions: 236 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 232 Number of HSP's successfully gapped: 2 Length of query: 529 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 439 Effective length of database: 1,193,720 Effective search space: 524043080 Effective search space used: 524043080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)