BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0092.p (297 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillu... 446 e-126 gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillu... 150 1e-37 gi|300313737|ref|YP_003777829.1| gluconolactonase [Herbaspirillu... 57 1e-09 >gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 302 Score = 446 bits (1147), Expect = e-126, Method: Compositional matrix adjust. Identities = 206/291 (70%), Positives = 245/291 (84%) Query: 6 IQIAFDTPMQLGECPLWHAGEGALYWIDIPGRAVHRLHESSKTHRSWPMPGEPGCIAIHA 65 + +AF PM LGE PLWHA E ALYWIDIPG+AVHRLH S H+ W +P EPG I HA Sbjct: 11 VTVAFPEPMLLGESPLWHAQEAALYWIDIPGKAVHRLHPESGQHKRWDLPEEPGSIVRHA 70 Query: 66 DGGLLVALRDGLFRLDTDSGDLTRLLDAPYDVSKLRFNDGRCDATGRFWVGTIYEPRDQE 125 GGL+VA+R G+F L+TD+G LT LL APYD S++RFNDGRCDA+GRFW GTIYEPRD++ Sbjct: 71 QGGLVVAIRTGMFHLNTDTGALTLLLAAPYDSSRIRFNDGRCDASGRFWCGTIYEPRDRD 130 Query: 126 LGSLFCFDHGQLRDAAHPVTTSNGVAFSTDKRRMFHANTPAHRIDVYDFDLTSGSTSNKR 185 G+L+CF+ LRDA H V TSNG+AFS+D+R M+HANTPAHRI++YD+DL +G+TSN R Sbjct: 131 GGTLYCFERNALRDAHHAVVTSNGLAFSSDQRLMYHANTPAHRINLYDYDLATGATSNCR 190 Query: 186 LFKQFDTDKTAPDYGGRPDGAAVDSENAYWCAMFEGGRVLRLSATGDILQEINIPARCPT 245 L +QFD+DK+APDYGGRPDGAAVDS+ AYWCAMFEGGRVLRLS G+ILQEI +PARCPT Sbjct: 191 LLRQFDSDKSAPDYGGRPDGAAVDSQGAYWCAMFEGGRVLRLSPQGEILQEIRLPARCPT 250 Query: 246 MIAFGGEDLRTLYITTARNGRSEAELAQYPHSGCVLSLRVDVAGLPEFAYR 296 M+AFGG+DLRTLYITT RNGRS AELAQYP SGCVL++RVDV G E++YR Sbjct: 251 MVAFGGDDLRTLYITTGRNGRSPAELAQYPESGCVLAVRVDVPGQLEYSYR 301 >gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 150 bits (379), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 98/297 (32%), Positives = 146/297 (49%), Gaps = 12/297 (4%) Query: 6 IQIAFDTPMQLGECPLWHAGEGALYWIDIPGRAVHRLHESSKTHRSWPMPGEPGCIAIHA 65 +Q+ D +LGE LW +++W DI + + RSW MP C A A Sbjct: 3 VQLLVDGHHELGEGVLWCDRSQSVFWTDIHASRLWNHDPQTGLTRSWGMPERLCCYAFTA 62 Query: 66 D-GGLLVALRDGLFRLDTDSGDLTRLLDAPYDVSKLRFNDGRCDATGRFWVGTIYEPRDQ 124 D LL+ L L + +G + + D+ R NDGRCD GRF GT+ E + Sbjct: 63 DPQQLLIGLESRLAFFNLSTGTIAPICRIEDDLPSTRLNDGRCDRQGRFVFGTLNEDAGR 122 Query: 125 E-LGSLFCF--DHGQLRDAAHPVTTSNGVAFSTDKRRMFHANTPAHRIDVYDFDLTSGST 181 + S + D R + + SN + FS D + M+H ++ A +I V D+D SG+ Sbjct: 123 APIASFYRLNTDLTLERLSLPSIAISNSICFSLDGKLMYHCDSMAGKIMVCDYDTASGAV 182 Query: 182 SNKRLFKQFDTDKTAPDYGGRPDGAAVDSENAYWCAMFEGGRVLRLSATGDILQEINIPA 241 +R+F D P G PDG+ +D+E W A + G RV+R + G I + + IP Sbjct: 183 GGQRVF----ADVAQP---GGPDGSTIDAEGYLWNAQWGGARVVRYAPDGRIDRVVAIPT 235 Query: 242 RCPTMIAFGGEDLRTLYITTARNGRSEAELAQYPHSGCVLSLRV-DVAGLPEFAYRG 297 P+ +AFGG TLY+TTA G S + A P +G + ++ + DV GLPE + G Sbjct: 236 SQPSCVAFGGAQFDTLYVTTAHEGMSAEQRAADPQAGALFAVALADVRGLPEVRFAG 292 >gi|300313737|ref|YP_003777829.1| gluconolactonase [Herbaspirillum seropedicae SmR1] Length = 306 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 70/277 (25%), Positives = 116/277 (41%), Gaps = 30/277 (10%) Query: 26 EGALYWIDIPGRAVHRLHESSKTHRSWPMPGEPGCIAIHADGGLLVA-LRDGLFRLDTDS 84 +G LY DIP + R+ + G P + I G +++ + GL +LD D+ Sbjct: 52 QGRLYVTDIPYGRIFRITMDGQWELVTEYDGWPNGLKIDKTGRIVITDYKRGLVQLDPDT 111 Query: 85 GDLTRLLDAPYDVSKLRFNDGRCDATGRFWVGTIYEPRDQE-LGSLFCF-DHGQLRDAAH 142 G +T LL+ ND G + + Q+ G ++ GQL + Sbjct: 112 GTVTPLLETVGSEGFKGVNDLVFGPKGEIYFTDQGQTGLQDPTGRVYRLAPDGQLTCLIN 171 Query: 143 PVTTSNGVAFSTDKRRMFHANTPAHRIDVYDFDLT-SGSTSNKRLFKQFDTDKTAPDYGG 201 + + NG+ + + A T A +I + L +G +F +GG Sbjct: 172 TIPSPNGIVYDPALNHLLVAVTRAQQI--WRIPLHHNGVVGKVGVFANL--------HGG 221 Query: 202 R--PDGAAVDSENAYWCAMFEGGRVLRLSATGDILQEINIPARCPTM-IAFGGEDLRTLY 258 PDG A+D+E+ + A G + RLS + L I A T +AFGG D ++L+ Sbjct: 222 MAGPDGLALDAESNLYIAHTGFGSIWRLSKFAEPLLRIRSSAGISTTNLAFGGPDGKSLF 281 Query: 259 ITTARNGRSEAELAQYPHSGCVLSLRVDVAGLPEFAY 295 IT ++ +G +L V VAGLP +++ Sbjct: 282 ITESQ-------------TGSILRADVPVAGLPMYSH 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,051,239 Number of extensions: 47198 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 3 Length of query: 297 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 212 Effective length of database: 1,217,395 Effective search space: 258087740 Effective search space used: 258087740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)