BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0090.p (218 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 166 2e-42 gi|300314015|ref|YP_003778107.1| hyperosmotically inducible peri... 83 2e-17 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 68 6e-13 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 64 8e-12 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 64 1e-11 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 166 bits (419), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 90/109 (82%), Positives = 100/109 (91%) Query: 109 RAVSKAKAMDLCLTGRMMDAQEAERAGLVSRVVAADKLMDEALAAAIVITEMSQPVAAMV 168 RAVSKAKAMDL LTGRMMDA+EAERAGLVSR+VAADKL++EA+AAAI+I EM + VA MV Sbjct: 149 RAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMMV 208 Query: 169 KDSVNRAYETTLAEGIKYERALFYSCFATEDQTEGMAAFLEKRTPSFKN 217 KDSVNRAYETTL+EG+KYERALFYSCFATEDQ EGM AFLEKR P FK+ Sbjct: 209 KDSVNRAYETTLSEGMKYERALFYSCFATEDQKEGMKAFLEKRQPVFKH 257 >gi|300314015|ref|YP_003778107.1| hyperosmotically inducible periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 82.8 bits (203), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 40/55 (72%), Positives = 47/55 (85%) Query: 39 TTQKAGAYVDDSVITAKVKAAFVEDSQVSALKIKVTTKKGIVTLAGPVPNADVAR 93 T QKAGAY+DDSVITAKVKAA VED QVSA+KI VTTK G+VTL G VP+A++ + Sbjct: 34 TAQKAGAYIDDSVITAKVKAALVEDKQVSAMKINVTTKDGVVTLKGSVPSAELGQ 88 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 68.2 bits (165), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 43/105 (40%), Positives = 56/105 (53%), Gaps = 2/105 (1%) Query: 114 AKAMDLCLTGRMMDAQEAERAGLVSRVVAADKLMDEALAAAIVITEMSQPVAAMVKDSVN 173 AK M LC G ++A+ A R GLV VV + + ALA A + S A K + Sbjct: 158 AKRMILC--GERVNAETALRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQ 215 Query: 174 RAYETTLAEGIKYERALFYSCFATEDQTEGMAAFLEKRTPSFKNQ 218 A L ++ ER LF F T+DQ EG+ AFLEKR+P +KNQ Sbjct: 216 GARHNPLGTILQTERELFVDLFDTQDQREGVQAFLEKRSPQWKNQ 260 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 64.3 bits (155), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 40/110 (36%), Positives = 56/110 (50%) Query: 109 RAVSKAKAMDLCLTGRMMDAQEAERAGLVSRVVAADKLMDEALAAAIVITEMSQPVAAMV 168 R V A+AM + L G + A++AER GL+ + V D+LM+EA A A A Sbjct: 154 RLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAAT 213 Query: 169 KDSVNRAYETTLAEGIKYERALFYSCFATEDQTEGMAAFLEKRTPSFKNQ 218 K ++ + TL E + ER + + D EG+ AFLEKR P F Q Sbjct: 214 KATLYASPAHTLPEQLDLERDTMRALGRSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 39/110 (35%), Positives = 61/110 (55%) Query: 109 RAVSKAKAMDLCLTGRMMDAQEAERAGLVSRVVAADKLMDEALAAAIVITEMSQPVAAMV 168 R V K AM + L+G + A +A +AGLV+ VV D ++ LA A I + S + Sbjct: 153 RTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLA 212 Query: 169 KDSVNRAYETTLAEGIKYERALFYSCFATEDQTEGMAAFLEKRTPSFKNQ 218 K+++ +++E L G+ +ER F A+ D+ EG+AAF EKR F + Sbjct: 213 KEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVFSGR 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.121 0.312 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 349,083 Number of extensions: 9674 Number of successful extensions: 60 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 5 Length of query: 218 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 136 Effective length of database: 1,231,600 Effective search space: 167497600 Effective search space used: 167497600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)