BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0089.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314016|ref|YP_003778108.1| two component transcription reg... 442 e-125 gi|300313916|ref|YP_003778008.1| two component response regulato... 194 7e-51 gi|300312042|ref|YP_003776134.1| response regulatory protein [He... 191 8e-50 gi|300312179|ref|YP_003776271.1| nif-specific regulatory protein... 190 1e-49 gi|300312654|ref|YP_003776746.1| C4-dicarboxylate transport resp... 179 2e-46 >gi|300314016|ref|YP_003778108.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 466 Score = 442 bits (1136), Expect = e-125, Method: Compositional matrix adjust. Identities = 217/259 (83%), Positives = 235/259 (90%) Query: 12 PEQLLESELFGHVKGAFTGAVEHRKGLFQAADGGTLFLDEIGDMPLPLQVKLLRVLQERT 71 PEQLLESELFGHVKGAFTGAVEHR+GLFQAA GGTLFLDEIGDMPLPLQVKLLRVLQE+T Sbjct: 205 PEQLLESELFGHVKGAFTGAVEHREGLFQAAHGGTLFLDEIGDMPLPLQVKLLRVLQEKT 264 Query: 72 VRPVGANDSTPVDVRILSATHRNLDAAMADGSFREDLYYRLNVVTLSLPLLVERREDIAL 131 VR VGAN + VDVRI+SATHR+L+AAM +G FREDLYYRLNVVTL LP L ERREDIAL Sbjct: 265 VRQVGANQAGEVDVRIISATHRDLEAAMREGQFREDLYYRLNVVTLHLPALEERREDIAL 324 Query: 132 LANHFLQAIAERYQKKLNGFAPDALEILMMASWPGNVRQLYNVVEQVCALSTAPLIPAAL 191 LANHFL+ IA +Y K+L GFAPDALEIL +A WPGNVRQLYNVVEQVCALSTAPLIPA+L Sbjct: 325 LANHFLKTIAAKYPKRLTGFAPDALEILSLAHWPGNVRQLYNVVEQVCALSTAPLIPASL 384 Query: 192 VQRALRVPALEVLRYADAKQRFERDYLIQLLKLTDGNVADAARLADRNRTEFYRLLQKNG 251 VQRALR P L++LRYA+AKQ+FERDYLI+LLKLTDGNV+DAARLADRNRTEFYRLLQKNG Sbjct: 385 VQRALRTPTLDLLRYAEAKQKFERDYLIRLLKLTDGNVSDAARLADRNRTEFYRLLQKNG 444 Query: 252 LDPRLFRGDEEDVAIERQE 270 LDPR FRG EE V RQE Sbjct: 445 LDPRRFRGGEEAVVAGRQE 463 >gi|300313916|ref|YP_003778008.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 448 Score = 194 bits (492), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 114/251 (45%), Positives = 151/251 (60%), Gaps = 14/251 (5%) Query: 12 PEQLLESELFGHVKGAFTGAVEHRKGLFQAADGGTLFLDEIGDMPLPLQVKLLRVLQERT 71 P +L+ESELFGHVKGAF+GA R G FQ ADGGTLFLDEIGDM L Q K+LRVLQER Sbjct: 197 PAELIESELFGHVKGAFSGATSPRLGRFQEADGGTLFLDEIGDMELATQAKILRVLQERE 256 Query: 72 VRPVGANDSTPVDVRILSATHRNLDAAMADGSFREDLYYRLNVVTLSLPLLVERREDIAL 131 + PVG N S VDVR+++ATHR+L + G FREDL+YRL V+T+ +P L ER DI Sbjct: 257 LTPVGGNQSVRVDVRVIAATHRDLQHEITQGRFREDLWYRLQVLTIMVPALRERPGDILT 316 Query: 132 LANHFLQAIAERYQKKLNGFAPDALEILMMASWPGNVRQLYNVVEQVCALSTAPLIPAAL 191 +A HFL+ + K+++ P A +L+ +WPGNVR+L N +E+ +S I A Sbjct: 317 IAEHFLRQLGGDAPKRIS---PAAARLLLQHAWPGNVRELRNTMERAAVMSHGLCIEAED 373 Query: 192 VQRALRVPALEVLR-----------YADAKQRFERDYLIQLLKLTDGNVADAARLADRNR 240 + + R PA A A ER + + L+ GN A+AAR +R Sbjct: 374 IGLSARPPAGAASAASAPAIDWDGPLAVALAEVERVMIERALQGAAGNRAEAARRLGISR 433 Query: 241 TEFYRLLQKNG 251 + YR L++ G Sbjct: 434 QQLYRRLEEAG 444 >gi|300312042|ref|YP_003776134.1| response regulatory protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 191 bits (484), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 100/239 (41%), Positives = 137/239 (57%) Query: 12 PEQLLESELFGHVKGAFTGAVEHRKGLFQAADGGTLFLDEIGDMPLPLQVKLLRVLQERT 71 PE LLESELFG+ KGAFTGA + G + A GGT FLDE+GD+P+PLQ KLLR LQER Sbjct: 209 PENLLESELFGYEKGAFTGAAKQTIGKVELAHGGTFFLDEVGDLPMPLQAKLLRFLQERV 268 Query: 72 VRPVGANDSTPVDVRILSATHRNLDAAMADGSFREDLYYRLNVVTLSLPLLVERREDIAL 131 + VG + PVDVR++ ATH+NL A G FREDLYYRL + + +P L ER D L Sbjct: 269 IERVGGHTEIPVDVRVICATHQNLKELTASGGFREDLYYRLCEIIIKIPALKERHGDAVL 328 Query: 132 LANHFLQAIAERYQKKLNGFAPDALEILMMASWPGNVRQLYNVVEQVCALSTAPLIPAAL 191 LA HF+Q + + + DA+ + WPGNVR++ N V + ++ I A Sbjct: 329 LARHFVQKFCAKEGRATLHLSQDAVAAIERYEWPGNVREMENCVMRAVIMADGQQITAED 388 Query: 192 VQRALRVPALEVLRYADAKQRFERDYLIQLLKLTDGNVADAARLADRNRTEFYRLLQKN 250 + ++ E L + ER +++ L DGN+ AA L +R Y L+ ++ Sbjct: 389 LGLSVEGREAEPLNLRQVRDEAERKAVVKALTRMDGNILKAAELLGVSRPTLYDLMSRH 447 >gi|300312179|ref|YP_003776271.1| nif-specific regulatory protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 190 bits (482), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 94/175 (53%), Positives = 120/175 (68%) Query: 13 EQLLESELFGHVKGAFTGAVEHRKGLFQAADGGTLFLDEIGDMPLPLQVKLLRVLQERTV 72 E LLESELFGH KGAFTGA RKG F+ A GGTLFLDEIG++ Q KLLRVLQER Sbjct: 265 ETLLESELFGHEKGAFTGAQGERKGRFELAHGGTLFLDEIGEISPAFQAKLLRVLQEREF 324 Query: 73 RPVGANDSTPVDVRILSATHRNLDAAMADGSFREDLYYRLNVVTLSLPLLVERREDIALL 132 VG + S VDVR+++AT+R+L+ A+A G FR DLYYR+NVV++ +P L ERREDI L Sbjct: 325 ERVGGSRSIKVDVRLVTATNRDLEKAVAKGEFRADLYYRINVVSIFIPPLRERREDIPYL 384 Query: 133 ANHFLQAIAERYQKKLNGFAPDALEILMMASWPGNVRQLYNVVEQVCALSTAPLI 187 HFL+ Q+ + +P A++++M WPGNVR+L N VE+ + LI Sbjct: 385 VEHFLEKFRVENQRAMVAMSPQAMKVMMNCYWPGNVRELENCVERTATMMRGDLI 439 >gi|300312654|ref|YP_003776746.1| C4-dicarboxylate transport response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 179 bits (455), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 104/236 (44%), Positives = 147/236 (62%), Gaps = 7/236 (2%) Query: 12 PEQLLESELFGHVKGAFTGAVEHRKGLFQAADGGTLFLDEIGDMPLPLQVKLLRVLQERT 71 PE + ESE+FGH GAFTGA + R G + A+GGTLFLDEI MPL LQVKLLRVLQER Sbjct: 208 PETVFESEIFGHEPGAFTGAQKRRIGKLEYANGGTLFLDEIESMPLALQVKLLRVLQERR 267 Query: 72 VRPVGANDSTPVDVRILSATHRNLDAAMADGSFREDLYYRLNVVTLSLPLLVERREDIAL 131 + +G N+S +D R+++A+ +L A+G FREDLYYR+ VV + LP L +RREDIAL Sbjct: 268 LERLGGNESVAIDCRVVAASKADLLKLAAEGQFREDLYYRIGVVAIDLPALNQRREDIAL 327 Query: 132 LANHFLQAIAERYQKKLNGFAPDALEILMMASWPGNVRQLYNVVEQVCALSTAPLIPAAL 191 L HF QA A RY+++ ++ ++ WPGNVR+L N ++ L + +A+ Sbjct: 328 LLAHFCQAAAVRYRRESPAWSAAQMQQWQQRDWPGNVRELRNFADR-WVLGVEQMAASAV 386 Query: 192 VQRALRVPALEVLRYADAKQRFERDYLIQLLKLTDGNVADAA-RLADRNRTEFYRL 246 + VP L L + ++FE + LK ++G+VA AA RL +T + ++ Sbjct: 387 ---SSAVPVLSSL--PEQVEQFEAGLIAAALKDSEGSVAVAAERLGIPKKTLYDKI 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,123 Number of extensions: 32556 Number of successful extensions: 113 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 12 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)