BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0069.p (283 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 226 2e-60 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 127 8e-31 gi|300310674|ref|YP_003774766.1| amidase family protein [Herbasp... 113 1e-26 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 105 4e-24 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 104 8e-24 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 226 bits (576), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 130/229 (56%), Positives = 156/229 (68%), Gaps = 5/229 (2%) Query: 1 VSIKDLFDVQGEVTAAGSKALADAPAAQADALAVTRLRHAGAVLLGRTNMSEFAFSGLGL 60 VS+KDLFDV GE T AGS L P A A A V L AGA+L+G+TNM+EFA+SGLG+ Sbjct: 74 VSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSGLGI 133 Query: 61 NPHYGTPRNP----LDGTRVTGGSTSGGAVSVAGDMAVMALGTDTGGSIRIPSAFCGLTG 116 NPHYGTP+N ++G R+ GGS+SG A+SV MA A+G+DTGGS+RIPSA GLTG Sbjct: 134 NPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNGLTG 193 Query: 117 FKPTARRVSLDGAVPLSTTLDSAGPLARSVDCCAIADAILSGE-TLATDAVPLAGLRFGV 175 FKPTA RVS+ G +PLS LDS GPLA SV CCAI DA+L+GE +A A PL GLR Sbjct: 194 FKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVAVLAHPLRGLRLLA 253 Query: 176 TEDFVNDGMDDAVRAAFDAALARLQKAGALIQRFSFPELKELPSINAGG 224 + V DGMDD V A+ AL +L AGA+I P L +INA G Sbjct: 254 PTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINAKG 302 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 127 bits (320), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 89/219 (40%), Positives = 126/219 (57%), Gaps = 3/219 (1%) Query: 1 VSIKDLFDVQGEVTAAGSKALADAPAAQADALAVTRLRHAGAVLLGRTNMSEFAFSGLGL 60 ++IKD DV G + AG+ AL + Q A V L AGA+ G+ NM E AF Sbjct: 71 IAIKDNIDVAGIASTAGTAALRNN-VPQRHATVVEYLFGAGALHAGKANMHELAFGITNN 129 Query: 61 NPHYGTPRNPLDGTRVTGGSTSGGAVSVAGDMAVMALGTDTGGSIRIPSAFCGLTGFKPT 120 N +G RNP D + GGS+ G +A +A A+GTDTGGS+RIP+A CG+ G +PT Sbjct: 130 NGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVGLRPT 189 Query: 121 ARRVSLDGAVPLSTTLDSAGPLARSVDCCAIADAILSG--ETLATDAVPLAGLRFGVTED 178 +RR S G VP+S T D+ GP+ARSV A+ D I++G ++ A+P + LR GV + Sbjct: 190 SRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPPSQLRLGVLRN 249 Query: 179 FVNDGMDDAVRAAFDAALARLQKAGALIQRFSFPELKEL 217 G+++ V A +AAL +L++AG + P L L Sbjct: 250 PSWQGLEEEVEANANAALMQLREAGVELVDVDLPTLAPL 288 >gi|300310674|ref|YP_003774766.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 72/147 (48%), Positives = 94/147 (63%) Query: 1 VSIKDLFDVQGEVTAAGSKALADAPAAQADALAVTRLRHAGAVLLGRTNMSEFAFSGLGL 60 V+IKD D+ G+ T GS ALADAP A A A V RL AG + R NM E A+ G+ Sbjct: 29 VAIKDCIDIAGQPTCGGSAALADAPPASAHADVVARLLEAGWQITARANMHELAYGMTGI 88 Query: 61 NPHYGTPRNPLDGTRVTGGSTSGGAVSVAGDMAVMALGTDTGGSIRIPSAFCGLTGFKPT 120 N GT NP D +TGGS+SG A +V + +++G+DTGGSIR+P+A CG+ GFKP+ Sbjct: 89 NEWNGTAVNPQDPACMTGGSSSGSASAVGAGLVDVSIGSDTGGSIRLPAACCGVIGFKPS 148 Query: 121 ARRVSLDGAVPLSTTLDSAGPLARSVD 147 RVS GA P ++LD GP +RS+D Sbjct: 149 FGRVSRAGAYPALSSLDCLGPFSRSMD 175 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 105 bits (262), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 93/238 (39%), Positives = 119/238 (50%), Gaps = 20/238 (8%) Query: 1 VSIKDLFDVQGEVTAAGSKALADAPAAQADALAVTRLRHAGAVLLGRTNMSEFAFSGLGL 60 + +KD +D T G+ ALA AQADA V RLR AGAV+LG+T M E A + Sbjct: 124 ILVKDNYDTVDMPTTGGTLALATL-QAQADAFQVKRLREAGAVILGKTTMHELAAGVTTV 182 Query: 61 NPHYGTPRNPLDGTRVTGGSTSGGAVSVAGDMAVMALGTDTGGSIRIPSAFCGLTGFKPT 120 + G RNP D R GGS+ G +VA A +G+DT GSIRIP+A L G + T Sbjct: 183 SSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLFGLRTT 242 Query: 121 ARRVSLDGAVPLSTTLDSAGPLARSVDCCAI-------ADAILSGETLATDAVP------ 167 S G +PLS+T D A PLARSV+ AI +D S A +P Sbjct: 243 RGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDANGHIPKSYRDG 302 Query: 168 -----LAGLRFGVTEDFVNDGMDDA-VRAAFDAALARLQKAGALIQRFSFPELKELPS 219 L G R GV +DA V AA + AL +L+ GA++ + PEL L S Sbjct: 303 LRADSLQGARIGVLRALFGAAPEDAEVSAAINKALQQLKDQGAIVTDVTIPELDGLLS 360 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 104 bits (260), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 84/227 (37%), Positives = 119/227 (52%), Gaps = 15/227 (6%) Query: 1 VSIKDLFDVQGEVTAAGSKALADAPAAQADALAVTRLRHAGAVLLGRTNMSEFAFSGLGL 60 ++ KD+F +G T AGS+ L D + DA V + AG V LG+ N EFA Sbjct: 71 IAHKDIFVTRGWRTTAGSRML-DNYVSPFDATVVEQFNAAGMVTLGKLNCDEFAMGSGNE 129 Query: 61 NPHYGTPRNPLDGTRVTGGSTSGGAVSVAGDMAVMALGTDTGGSIRIPSAFCGLTGFKPT 120 N ++G NP D V GGS+ G A +VA +A A TDTGGSIR P++ CG+TG KPT Sbjct: 130 NSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASLCGVTGIKPT 189 Query: 121 ARRVSLDGAVPLSTTLDSAGPLARSVDCCAIADAILSG--ETLATDAV------------ 166 VS G + +++LD GP+A++ + C + ++G E +T Sbjct: 190 YGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPKEDFTRSLND 249 Query: 167 PLAGLRFGVTEDFVNDGMDDAVRAAFDAALARLQKAGALIQRFSFPE 213 L GLR GV ++F G+ V AA AAL+ +K GA + S P+ Sbjct: 250 SLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPK 296 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 809,164 Number of extensions: 29384 Number of successful extensions: 117 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 11 Length of query: 283 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 199 Effective length of database: 1,222,130 Effective search space: 243203870 Effective search space used: 243203870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)