BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0054.p (203 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313194|ref|YP_003777286.1| diadenosine tetraphosphate (Ap4... 161 4e-41 >gi|300313194|ref|YP_003777286.1| diadenosine tetraphosphate (Ap4A) hydrolase [Herbaspirillum seropedicae SmR1] Length = 150 Score = 161 bits (407), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 74/105 (70%), Positives = 88/105 (83%) Query: 2 SMANACDLCDGDGGELLFNNGSLRVVLVDEPAYPGFCRVIWNGHVKEMTDLAPADRSAVM 61 S+ AC+LC GDGGE+L+ + RVVLVDE YPGFCRVI + HVKEMTDLAPA R+ + Sbjct: 4 SVVPACELCAGDGGEVLWQHPVFRVVLVDEADYPGFCRVICHEHVKEMTDLAPAQRTLMA 63 Query: 62 SAVWAVETAVREVMQPEKMNVASLGNMTPHVHWHVIPRYLDDAHF 106 +AVWAVE A+REVMQPEK+N+A+LGNMTPHVHWHVIPR+ DD HF Sbjct: 64 NAVWAVEAALREVMQPEKVNLATLGNMTPHVHWHVIPRFTDDRHF 108 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 678,344 Number of extensions: 26895 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 61 Number of HSP's successfully gapped: 1 Length of query: 203 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 122 Effective length of database: 1,236,335 Effective search space: 150832870 Effective search space used: 150832870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)