BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0053.p (1340 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum ... 2549 0.0 gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 96 1e-20 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 84 5e-17 gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 73 2e-13 >gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1333 Score = 2549 bits (6607), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1231/1342 (91%), Positives = 1289/1342 (96%), Gaps = 11/1342 (0%) Query: 1 MNAPAKIQALLTDAPQGASPTRLREIPYNYTSFSDREIVIRLLGEDAWRVLDELRSKRQT 60 MNAPA+IQALLTDAP GA+P RLREIPYNYTSFSDREIVIRLLGE++W +LDELR KRQT Sbjct: 1 MNAPAQIQALLTDAPHGATPPRLREIPYNYTSFSDREIVIRLLGEESWSLLDELRGKRQT 60 Query: 61 GRSARMLYEVLGDIWVVRRNPYLQDDMLDNPKRRAALIEALHHRLAEVDKRRIDVNQSES 120 GRSARMLYEVLGDIWVVRRNPYLQDD+LDNPKRRAALIEAL+HRL EVDKRR+ +Q+E+ Sbjct: 61 GRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRAALIEALNHRLGEVDKRRLATDQAEA 120 Query: 121 GDAAAADSARRSANVELLIKAARKAIADFGEEFKHTYDLRKRATKVLGRYTAKDNIKFDG 180 GDA A RRSA+VE L+KAA+KAIADF EEF+ TYDLRKRATKVLGR+TAKDNIKFDG Sbjct: 121 GDAEAQ---RRSASVEALLKAAKKAIADFAEEFRQTYDLRKRATKVLGRFTAKDNIKFDG 177 Query: 181 LSRVSHVTDATDWRVEYPFVVLTPDSEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPL 240 LSRVSHVTDATDWRVEYPFVVLTPD+EDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPL Sbjct: 178 LSRVSHVTDATDWRVEYPFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPL 237 Query: 241 TPMSAVINTEKLEQLGAVEMEVLPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPT 300 TPMSAVINTEKLEQLGAVEME+LPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPT Sbjct: 238 TPMSAVINTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPT 297 Query: 301 SAEASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEL 360 SAEASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVE+ Sbjct: 298 SAEASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEV 357 Query: 361 ASFKLEWSHPGEKGQKTEVFKTEILEIAGRKFRKEGLGKDVTDKFLSGLPGIQKEGCDGL 420 A FKLEWSHPGEKGQKTEVFKTEILEI+G+KFRKEGLGKDVTDKFLSGLPG+QKEGCDGL Sbjct: 358 ARFKLEWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGL 417 Query: 421 ITSARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKNYLDAETKKGGAILAGLEHLDER 480 ITSARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIK+YLDAETKKGGAILAGLEHLDER Sbjct: 418 ITSARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDER 477 Query: 481 YLRAVGYATKSKRGVLPKMVLIGDIVGDDENAVAQAASEVIRMANNRVGEGFVAISPEAR 540 YLRAVGYATKSKRGVLPKMVLIGDIVGDDENAVA AASEVIRMANNRVGEGFVA+SPEAR Sbjct: 478 YLRAVGYATKSKRGVLPKMVLIGDIVGDDENAVAAAASEVIRMANNRVGEGFVAVSPEAR 537 Query: 541 KKFWLDRSRTAAIAKHTNAFKINEDVVIPLPRMGEYTDGIERINIELSIKNKLQFLDELN 600 KKFWLDRSRTAAIAKHTNAFKINEDVVIPL RMGEYTDGIERINIELSIKNKLQ L EL+ Sbjct: 538 KKFWLDRSRTAAIAKHTNAFKINEDVVIPLNRMGEYTDGIERINIELSIKNKLQLLAELD 597 Query: 601 AFFVKGNLPLGKSDDAEGEDIPAAEMLEDRVHQAEELIARTQARWSYLLANLDKPLTVAK 660 +FFVKGNLPLGKSDDAEG+DIPAAEMLEDRVHQAE L+ +T ARWSYLLANLDKPL AK Sbjct: 598 SFFVKGNLPLGKSDDAEGDDIPAAEMLEDRVHQAESLLEQTHARWSYLLANLDKPLGEAK 657 Query: 661 GEMAALGLDKLLPVFDQRLLDQPDANVFHVVQDRTVRVSWKQEVRAQLRYRAQLRQIFSG 720 GE+AALGL+K+LPVF+QRL+DQP+A VFHVVQDRTVR+SWKQEV RAQLRQIFSG Sbjct: 658 GELAALGLEKMLPVFEQRLVDQPEAAVFHVVQDRTVRISWKQEV------RAQLRQIFSG 711 Query: 721 GAFKLILEECNAVHKRVLRGRVFVALHMHAGDGNVHTNLPVNSDHYQMLQDAHEAVARIM 780 AFKLILEEC A+HKRVLRGRVFVALHMHAGDGNVHTN+PVNSDHY+MLQDAH AVARIM Sbjct: 712 AAFKLILEECQAIHKRVLRGRVFVALHMHAGDGNVHTNIPVNSDHYEMLQDAHVAVARIM 771 Query: 781 TLARSLDGVISGEHGIGITKLEFLTEDEISEFRDYKLRVDPEGRFNKGKLLNLPGFEADL 840 LARSL+GVISGEHGIGITKLEFLTEDEI EFR+YK RVDPEGRFNKGKLLNLPG EADL Sbjct: 772 KLARSLNGVISGEHGIGITKLEFLTEDEIGEFREYKKRVDPEGRFNKGKLLNLPGMEADL 831 Query: 841 SNAYTPSFGLMGHESLIMQQSDIGAIANSVKDCLRCGKCKPVCATHVPRANLLYSPRNKI 900 SNAYTPSFGLMGHESLIMQQSDIGAIA+SVKDCLRCGKCKPVC+THVPRANLLYSPRNKI Sbjct: 832 SNAYTPSFGLMGHESLIMQQSDIGAIASSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKI 891 Query: 901 LATSLLVEAFLYE--SXRGVSIKHWEEFEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRN 958 LATSLLVEAFLYE + RGVSIKHWEEFEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRN Sbjct: 892 LATSLLVEAFLYEEQTRRGVSIKHWEEFEDVADHCTVCHKCVTPCPVDIDFGDVSMNMRN 951 Query: 959 LLRKMGKKSFNPGTATAMFFLNATDPATINATRKVMTDWGFKAQRLGNDIFKKFARKQTQ 1018 LLRKMGKKSFN GT AMFFLNATDPATINATRKVMT WGFKAQRLGND+ KKFA+KQTQ Sbjct: 952 LLRKMGKKSFNAGTNAAMFFLNATDPATINATRKVMTQWGFKAQRLGNDLMKKFAKKQTQ 1011 Query: 1019 KPAATVGKAPIKEQVIHFINKKMPGNLPKKTARALLDIEDDKIVPIIRDPKTTTAETEAV 1078 KP ATVGK P+KEQVIHFINKKMPGNLPKKTARALLDIEDDKIVPIIR+PKTTTA+TEAV Sbjct: 1012 KPPATVGKPPVKEQVIHFINKKMPGNLPKKTARALLDIEDDKIVPIIRNPKTTTADTEAV 1071 Query: 1079 FYFPGCGSERLFSQVGLATQAMLWNVGVQTVLPPGYLCCGYPQRGSGDFDKAEKIITDNR 1138 FYFPGCGSERLFSQVGLATQAMLWNVGVQTVLPPGYLCCGYPQRG+GDF+K EKIITDNR Sbjct: 1072 FYFPGCGSERLFSQVGLATQAMLWNVGVQTVLPPGYLCCGYPQRGTGDFEKGEKIITDNR 1131 Query: 1139 VLFHRMANTLNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGCRIIDIHEYLLEKGVRLEGV 1198 VLFHRMANTLNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGCRIIDIHEYLLEKGV+LEGV Sbjct: 1132 VLFHRMANTLNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGCRIIDIHEYLLEKGVKLEGV 1191 Query: 1199 TGTRYMYHDPCHSPMKLQDPLKTVNSLITTIDAQKIEKNDRCCGESGTFGVSRPDVSTQV 1258 TGTRYMYHDPCHSPMK QDPLKTVNSLITTIDAQKIEKNDRCCGESGTFGVSRPDVSTQV Sbjct: 1192 TGTRYMYHDPCHSPMKQQDPLKTVNSLITTIDAQKIEKNDRCCGESGTFGVSRPDVSTQV 1251 Query: 1259 RFRKEEEMRKGADKVRADGFDGDVKILTSCPSCFQGLSRYNEDSGTTADYIVVEMAKHLL 1318 RFRKEEEMRKG+DKVRADGF GDVKILTSCPSCFQGLSRYNED+GTTADYIVVEMA+HLL Sbjct: 1252 RFRKEEEMRKGSDKVRADGFTGDVKILTSCPSCFQGLSRYNEDAGTTADYIVVEMARHLL 1311 Query: 1319 GENWLPDYVARANNGGIERVLV 1340 GENW+P+YV RANNGGIER+LV Sbjct: 1312 GENWMPEYVERANNGGIERILV 1333 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 96.3 bits (238), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 102/392 (26%), Positives = 157/392 (40%), Gaps = 76/392 (19%) Query: 196 EYPFVVLTPDSEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPMSAVINTEKLEQL 255 + P VV+ P++E ++ ++K C L + I+PRG GTG +GGA P+ V++T K Q+ Sbjct: 50 QLPMVVVLPENEAQVVAIMKTCHALQVQIVPRGAGTGLSGGATPIAD-GVVVSTAKFNQI 108 Query: 256 GAVEMEVLPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASCIGGNVAMNA 315 ++M DK T GV +S+AA G +A DP+S A IGGNVA N+ Sbjct: 109 --LQM------DKYSRTAVVQPGVRNLAISEAAAPHGLYYAPDPSSQIACSIGGNVAENS 160 Query: 316 GGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVELASFKLEWSHPGEKGQ 375 GG + +G + N+ RMV +G+ VEL S L+ Sbjct: 161 GGVHCLKYGLTVHNVLRVRMVTIEGEV---------------VELGSGALDAP------- 198 Query: 376 KTEVFKTEILEIAGRKFRKEGLGKDVTDKFLSGLPGIQKEGCDGLITSARWILHKMPKQT 435 G D+ F I EG G++T L P+ Sbjct: 199 ----------------------GLDLLAVF------IGSEGMLGVVTEVTVKLVPKPQAA 230 Query: 436 RTVCLEFFGQARDAIPSIVEIKNYLDAETKKGGAILAGLEHLDERYLRAVGYATKSKRGV 495 R + F D + + N + A G I AGLE +D+ R V K+ + Sbjct: 231 RVIMASF----DDVVKGGNAVANVIAA-----GIIPAGLEMMDKTSSRMVEPFVKAGYDI 281 Query: 496 LPKMVLIGDIVGDDENAVAQAASEVIRMANNRVGEGFVAI----SPEARKKFWLDRSRTA 551 + +L+ + + V + E+ RM + G AI S R +FW R Sbjct: 282 DAEAILL----CESDGTVEEVEEEIGRMTDVLNASGATAIACSQSEAERLRFWSGRKNAF 337 Query: 552 AIAKHTNAFKINEDVVIPLPRMGEYTDGIERI 583 A + D IP ++ + GI + Sbjct: 338 PAAGRISPDYYCMDGTIPRKKLAQVLLGIAEM 369 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 84.3 bits (207), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 102/396 (25%), Positives = 155/396 (39%), Gaps = 75/396 (18%) Query: 183 RVSHVTDATDWRVEYPFVVLTPDSEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTP 242 R H D + + P V+ S +E+A VK C + IIP G GT G + L Sbjct: 37 REHHGRDESSYDPMLPDAVVFAHSTEEVAAFVKLCSQYDTPIIPYGAGTSLEGHVLALQG 96 Query: 243 MSAVINTEKLEQLGAVEMEVLPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSA 302 ++ ++ Q+ AV E L T AGV K+++ + G F +DP A Sbjct: 97 -GVTVDLSQMNQVLAVHAEDL--------TATVQAGVTRKQLNQEIKDTGLFFPIDP-GA 146 Query: 303 EASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVELAS 362 +AS +GG + A G AV +GT +N + +V QG Sbjct: 147 DAS-LGGMASTRASGTNAVRYGTMKENTLTLTVVTAQG---------------------- 183 Query: 363 FKLEWSHPGEKGQKTEVFKTEILEIAGRKFRKEGLGKDVTDKFLSGLPGIQKEGCDGLIT 422 E+ KT G + +K G D+T + + EG G+IT Sbjct: 184 ---------------EIIKT------GTRAKKSSAGYDLTRVY------VGSEGTLGIIT 216 Query: 423 SARWILHKMPKQTRTVCLEFFGQARDAIPSIVEIKNYLDAETKKGGAILAGLEHLDERYL 482 L+ P+ F P++ + N + +T + G LA +E LDE + Sbjct: 217 EVTVRLYPQPEAISAAICSF--------PTVADAVNTV-IQTIQMGVPLARVELLDENGV 267 Query: 483 RAVGYATKSKRGVLPKMVLIGDIVGDDENAVAQAASEVIRMANNRVGEGFV-AISPEARK 541 RA+ K V P +L+ + G EN V + A V +A GF A PE R Sbjct: 268 RAINAHDKLSLPVNP--LLLFEFHG-SENGVKEQAELVQDIAKEFHALGFEWATRPEDRT 324 Query: 542 KFWLDRSRT--AAIAKHTNAFKINEDVVIPLPRMGE 575 + W R A + A I+ D +P+ R+ E Sbjct: 325 RLWTARHNAYFALLQLRPGARAISTDCCVPISRLAE 360 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 72.8 bits (177), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 94/398 (23%), Positives = 155/398 (38%), Gaps = 80/398 (20%) Query: 191 TDWRVEY---PFVVLTPDSEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPMSAVI 247 TD R Y VL P E+A +V+ C + + ++P+GG TG G++P +AV+ Sbjct: 36 TDQRGRYTGRALAVLRPADSAEVAAVVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVV 95 Query: 248 -NTEKLEQLGAVEMEVLPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASC 306 + +L ++ AV D TI +G V + + + A AG +F + +AE SC Sbjct: 96 LSLRRLNRIRAV--------DALNNTITVESGCVLQHLQEQAAAAGRLFPLS-LAAEGSC 146 Query: 307 -IGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVELASFKL 365 IGGN++ NAGG + +G + +V QG+ ++S K Sbjct: 147 TIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEI-----------------MSSLK- 188 Query: 366 EWSHPGEKGQKTEVFKTEILEIAGRKFRKEGLGKDVTDKFLSGLPGIQKEGCDGLITSAR 425 RK+ G D+ D F I EG G+IT+A Sbjct: 189 -------------------------GLRKDNTGYDLRDLF------IGAEGTLGVITAAV 217 Query: 426 WILHKMPKQTRTVCLEFFGQARDAIPSIVEIKNYLDAETKKGGAILAGLEHLDERYLRAV 485 L P+ T A A+P + L G L G E + + L+ V Sbjct: 218 MKLFPQPRAQVT--------ALAALPHPAQALQLLQLAQAHCGPALTGFELMSDFCLQLV 269 Query: 486 GYATKSKRGVL----PKMVLIGDIVGDDENAVAQAASEVI---RMANNRVGEGFVAISPE 538 +R P+ VL+ ++ + A+A E + + V + +A S Sbjct: 270 RKHFPEQRLPFDRPHPQYVLL-ELSDSESEEHARALFEGLIGTALEQGLVDDAVIAASLA 328 Query: 539 ARKKFWLDRSRTAAIAKHTNAFKINEDVVIPLPRMGEY 576 + W R ++ H I D+ +P+ R+ E+ Sbjct: 329 QSRALWRLRESISSAQAHEGK-NIKHDISVPISRIAEF 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,045,917 Number of extensions: 167780 Number of successful extensions: 463 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 456 Number of HSP's successfully gapped: 4 Length of query: 1340 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1243 Effective length of database: 1,160,575 Effective search space: 1442594725 Effective search space used: 1442594725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 137 (57.4 bits)