BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0031.0052.p
         (317 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309886|ref|YP_003773978.1| divalent heavy-metal cations tr...   189   3e-49

>gi|300309886|ref|YP_003773978.1| divalent heavy-metal cations
           transporter protein [Herbaspirillum seropedicae SmR1]
          Length = 309

 Score =  189 bits (480), Expect = 3e-49,   Method: Compositional matrix adjust.
 Identities = 120/258 (46%), Positives = 158/258 (61%), Gaps = 5/258 (1%)

Query: 59  LTHPEVKMALLGSSAAAGLTALGALGVLFTRRISERTQDVLLGYGGGIMLAASVFSLIIP 118
           +  P +  A  G  AAA  TA G L + F+RR S R  D   G+G G+MLAASVFSLI+P
Sbjct: 56  VRDPRIWHAYCGGMAAAAATAAGTLPLFFSRRFSARMADCCTGFGAGVMLAASVFSLILP 115

Query: 119 AMNQAQALGYEKTGAVLLTATGIMLGGLFVLAINHLIPHQHFIAPHPAIQTMRTSRIWMF 178
           A+   +  G  +  A L+    ++ G L VL +          A   A++     R+W+F
Sbjct: 116 ALQSVRTAGMGQGQASLVIGASVVAGALLVLLLQRAGQGHDDAARQAALR-----RVWLF 170

Query: 179 VMAVTIHNFPEGLAIGVGFGGDDMGKAIALATGIGIQDIPEGLVVALALRTLGYSPWKSA 238
           V+ V +HN PEGLA+GV +GG    +A  L  GI +QDIPEG++VA ALR +GYS   + 
Sbjct: 171 VLTVGLHNLPEGLAVGVAYGGVPAEQASVLTFGIALQDIPEGMIVATALRGIGYSRADAI 230

Query: 239 GAGVLSGLVEPIGGVLGALATGVSATALPWALAGAGGAMLFVISHEVIPESHRQGHETQA 298
           G G+LSGLVEP+  V GA    +S+  LPWAL GA GAMLFV++HEVIP+ HR+     A
Sbjct: 231 GCGILSGLVEPLAAVAGAAVVALSSALLPWALGGAAGAMLFVLAHEVIPDPHRRHDALAA 290

Query: 299 TLGLLAGFVTMMLLDTLL 316
           T  L+AGFV MM+LDT L
Sbjct: 291 TCCLIAGFVLMMVLDTAL 308


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.139    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 850,063
Number of extensions: 33216
Number of successful extensions: 162
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 160
Number of HSP's successfully gapped: 2
Length of query: 317
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 232
Effective length of database: 1,217,395
Effective search space: 282435640
Effective search space used: 282435640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 130 (54.7 bits)