BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0050.p (183 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 337 3e-94 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 136 9e-34 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 122 3e-29 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 59 2e-10 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 337 bits (865), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 160/175 (91%), Positives = 167/175 (95%) Query: 1 MTTDYLQKILTARVYDVAMETPLEIANTLSQRMENRIYFKREDMQSVFSFKLRGAYNKMA 60 M+ DYL+KILTARVYDVA ETPLE+ANTLSQR++NRIYFKREDMQSVFSFKLRGAYNKMA Sbjct: 1 MSNDYLKKILTARVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMA 60 Query: 61 HLPPAQLKRGVICASAGNHAQGVALSAAKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLF 120 HL PAQLKRGVICASAGNHAQGVALSAA+LGCRAVIVMPTTTP VKI+AVK RGGEVVLF Sbjct: 61 HLTPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLF 120 Query: 121 GDSFTDAYNHALTLEKKLKLTFVHPFDDPYVIAGQGTVGMEILRQHADPIHAIFV 175 GDSFTDAY HALTLEKK KLTFVHPFDDPYVIAGQGTVGMEILRQH DPIHAIFV Sbjct: 121 GDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFV 175 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 136 bits (343), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 74/151 (49%), Positives = 96/151 (63%), Gaps = 1/151 (0%) Query: 19 METPLEIANTLSQRMENRIYFKREDMQSVFSFKLRGAYNKMAHLPPAQL-KRGVICASAG 77 +ETP + TLSQ + +++ K E++Q SFK RGA NK+ + + RGV+ SAG Sbjct: 19 LETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVDVAASGGGARGVVAMSAG 78 Query: 78 NHAQGVALSAAKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLFGDSFTDAYNHALTLEKK 137 NHAQGVA A +LG A IVMP TP VKIE +G G VVL GD+ A HA L +K Sbjct: 79 NHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHGDTLEAARTHAYELAEK 138 Query: 138 LKLTFVHPFDDPYVIAGQGTVGMEILRQHAD 168 LTFVHP+DD +IAGQGTV +E+++ D Sbjct: 139 EGLTFVHPYDDEAIIAGQGTVALEMMKAVPD 169 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 122 bits (305), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 66/163 (40%), Positives = 93/163 (57%), Gaps = 1/163 (0%) Query: 13 RVYDVAMETPLEIANTLSQRMENRIYFKREDMQSVFSFKLRGAYNKMAHLPPAQLKRGVI 72 R+ A TP+ + T + +++FK E+ Q + +FK RGA N + Q K GVI Sbjct: 22 RIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALTQFDATQRKHGVI 81 Query: 73 CASAGNHAQGVALSAAKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLFGDSFTDAYNHAL 132 S+GNHAQG AL+A LG AVIVMP P+ K+ A +G G EVV + D Sbjct: 82 TFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTYDRYTEDREAIGA 141 Query: 133 TLEKKLKLTFVHPFDDPYVIAGQGTVGMEILRQHADPIHAIFV 175 L + K+T + P+D P+V+AGQGT +E+L Q P+ A+FV Sbjct: 142 RLAAERKMTLIPPYDHPHVMAGQGTATLELL-QETGPLDALFV 183 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 58.9 bits (141), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 47/154 (30%), Positives = 77/154 (50%), Gaps = 5/154 (3%) Query: 29 LSQRMENRIYFKREDMQSVFSFKLRGAYNKMAHLPPAQLK-RGVICASAGNHAQGVALSA 87 L+Q + ++ K E+ +FK+RG + HL Q + GV+ A+ GNH Q V L+A Sbjct: 37 LNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAA 96 Query: 88 AKLGCRAVIVMPTTTPSVKIEAVKGRGGEVVLFGDSFTDAYNHALTLEKKLKLTFVHPFD 147 +LG A IV+P K A++ G E++ G F ++ +A L + +L + P Sbjct: 97 RRLGISATIVVPQGNSREKNAAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMI-PSL 155 Query: 148 DPYVIAGQGTVGMEILRQHADP-IHAIFVAIGGG 180 ++AG + ME+ A P + + V IG G Sbjct: 156 HRDLVAGVASYWMELFA--AQPGLDVVLVPIGQG 187 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 565,245 Number of extensions: 22247 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 55 Number of HSP's successfully gapped: 4 Length of query: 183 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 103 Effective length of database: 1,241,070 Effective search space: 127830210 Effective search space used: 127830210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)