BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0046.p (180 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313198|ref|YP_003777290.1| hypothetical protein Hsero_3909... 181 3e-47 >gi|300313198|ref|YP_003777290.1| hypothetical protein Hsero_3909 [Herbaspirillum seropedicae SmR1] Length = 291 Score = 181 bits (459), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 86/124 (69%), Positives = 100/124 (80%), Gaps = 5/124 (4%) Query: 1 MNHAN-----TNNSPDTSPLGKSSAYPDVYDPALLFPIPRQPKRDELGVTGTLPFFGVDI 55 M+H N ++SP++SPLGKSS YP YDP+LLFPIPR PKR EL ++GTLPFFGVD+ Sbjct: 1 MDHTNPSASPASHSPESSPLGKSSQYPTHYDPSLLFPIPRAPKRAELQISGTLPFFGVDL 60 Query: 56 WNAYEVSWLNQRGKPQIAIATFMIQADSPNIIESKSFKLYLNSFNQTRLEDQQALVALLQ 115 W AYE+SWLN RGKPQIAIATFM+ ADSPNIIESKSFKLYLNSFNQ RLE L+ ++ Sbjct: 61 WTAYEISWLNLRGKPQIAIATFMVPADSPNIIESKSFKLYLNSFNQERLESADQLIDKMR 120 Query: 116 KDLS 119 DLS Sbjct: 121 TDLS 124 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 502,857 Number of extensions: 18758 Number of successful extensions: 41 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 41 Number of HSP's successfully gapped: 1 Length of query: 180 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 101 Effective length of database: 1,245,805 Effective search space: 125826305 Effective search space used: 125826305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)