BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0028.p (1561 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313212|ref|YP_003777304.1| glutamate synthase large subuni... 2960 0.0 gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspiril... 132 2e-31 >gi|300313212|ref|YP_003777304.1| glutamate synthase large subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1558 Score = 2960 bits (7673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1441/1568 (91%), Positives = 1497/1568 (95%), Gaps = 17/1568 (1%) Query: 1 MHAQGLYDPANEHDACGVGFIAHIKGKKNHSIVEQGLLILKNLDHRGAVGADPLMGDGAG 60 MHAQGLYDPANEHDACGVGFIAHIKGKK+HSIV+QGLLILKNLDHRGAVGADPLMGDGAG Sbjct: 1 MHAQGLYDPANEHDACGVGFIAHIKGKKSHSIVDQGLLILKNLDHRGAVGADPLMGDGAG 60 Query: 61 ILIQIPDQYYREEMSRQGVELPPPGEYGVGMVFLPKENASRIACEQEIERSVLAEGQVVL 120 ILIQIPDQYYREEM++QGV+LPPPGEYGVGMVFLPKENASRIACEQEIER+VLAEGQ+VL Sbjct: 61 ILIQIPDQYYREEMTKQGVDLPPPGEYGVGMVFLPKENASRIACEQEIERAVLAEGQIVL 120 Query: 121 GWRNVPVDMDMPMSPTVREKEPVIRQIFIGRGPDIMVTDALERKLYVIRKSSGHAIQALN 180 GWR+VPVD DMPMSPTVREKEPVIRQIFIGRGPD+MVTDALERKLYVIRKSSGHAIQALN Sbjct: 121 GWRDVPVDRDMPMSPTVREKEPVIRQIFIGRGPDVMVTDALERKLYVIRKSSGHAIQALN 180 Query: 181 LIHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQDPRCVSALALVHQX-------EWP 233 L+HGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQD RCVSALALVHQ EWP Sbjct: 181 LLHGKEFFVPSMSARTVVYKGLLLADQVGVYYKDLQDERCVSALALVHQRFSTNTFPEWP 240 Query: 234 LAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVLGDDLKKLFPLIYEGQSDTACFDNA 293 LAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVLG DLKKLFPLIYEGQSDTA FDNA Sbjct: 241 LAHPYRLIAHNGEINTVKGNFNWMRAREGVMQSAVLGADLKKLFPLIYEGQSDTASFDNA 300 Query: 294 LELLLMAGYPLAQAMMMMVPEAWENHTLMDDNRRAFYEYHAAMMEPWDGPAALAFTDGRH 353 LELL+MAGYPL QAMMMMVPEAWENHT MDDNRRAFYEYHAAMMEPWDGPAALAFTDGRH Sbjct: 301 LELLVMAGYPLPQAMMMMVPEAWENHTSMDDNRRAFYEYHAAMMEPWDGPAALAFTDGRH 360 Query: 354 IGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPESKIIQKWRLQPGKMFLIDLDAGRI 413 IGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPESKIIQKWRLQPGKMFLIDLDAGRI Sbjct: 361 IGGTLDRNGLRPARYIVTDDDLVVMASESGVLPIPESKIIQKWRLQPGKMFLIDLDAGRI 420 Query: 414 IDDKELKDTYANAKPYKQWISSVRVKLDELKAEPVESGSTIPLLDRQQVFGYTQEDVKFL 473 IDDKELKDTYANAKPYKQWI+SVRVKLDELK EP + S++ LLD QQVFGYTQEDVKFL Sbjct: 421 IDDKELKDTYANAKPYKQWINSVRVKLDELKEEPRQPSSSLKLLDLQQVFGYTQEDVKFL 480 Query: 474 MAPMASAGEEAIGSMGNDSPLAVMSNKNKTLYNYFKQLFAQVTNPPIDPIREAMVMSLVS 533 MAPMAS GEEAIGSMGNDSPLAVMSNKNK LYNYF+QLFAQVTNPPIDPIREA+VMSLVS Sbjct: 481 MAPMASGGEEAIGSMGNDSPLAVMSNKNKPLYNYFRQLFAQVTNPPIDPIREALVMSLVS 540 Query: 534 FIGPRPNLLDTNNINPPMRLEVSQPILDYADIAKLRNISAHTGGKFKSHELNICYPVAWG 593 FIGP+PNLLDTNNINPPMRLEVSQP+LDY DIAKLRNISAH+GGKFKS+ELNICYPVAWG Sbjct: 541 FIGPKPNLLDTNNINPPMRLEVSQPVLDYDDIAKLRNISAHSGGKFKSYELNICYPVAWG 600 Query: 594 KEGIEARLASLCAKAVDAVKSGHNILIISDRKVDSEQVAIPALLATSAIHLHLVSKGLRT 653 KEGIEARLASLCAKAVDAVKSGHNILIISDRKVD+EQ+ IPALLATSAIH HLVSKGLRT Sbjct: 601 KEGIEARLASLCAKAVDAVKSGHNILIISDRKVDAEQLPIPALLATSAIHQHLVSKGLRT 660 Query: 654 STGLVVDTGSARETHHFALLAGYGAEAIHPYLAMDTLSEMAKGLPGELSGEKAIYNFQKA 713 STGLVV+TGSARETHHFALLAGYGAEAIHPYLAMDTL++MAKGLPG+LS EKAIYNFQKA Sbjct: 661 STGLVVETGSARETHHFALLAGYGAEAIHPYLAMDTLADMAKGLPGDLSPEKAIYNFQKA 720 Query: 714 IGKGLLKVMSKMGISTYMSYCGAQIFEAIGLNKALVDKYFKGTASNVEGIGVFEVAEEAL 773 +GKGLLKVMSKMGISTYMSYCGAQIFEAIGL+KALVDKYFKGTASNVEGIGVFEVAEEAL Sbjct: 721 VGKGLLKVMSKMGISTYMSYCGAQIFEAIGLSKALVDKYFKGTASNVEGIGVFEVAEEAL 780 Query: 774 RLHRAAFSADPVLANALDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRANNFNSYKEYAQI 833 RLH AAFSADPVLANALDAGGEYAFRIRGEEHMWTPDAIAKLQHSTR+N++N+YKEYAQI Sbjct: 781 RLHTAAFSADPVLANALDAGGEYAFRIRGEEHMWTPDAIAKLQHSTRSNSYNTYKEYAQI 840 Query: 834 INDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAI 893 INDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAI Sbjct: 841 INDQSKRHMTLRGLFEFKIDPSKAISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAI 900 Query: 894 AMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATLASEIGKDVIEVDIPLQAGDSLRSR 953 AMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATLASEIGK+ IEVDIPL GDSLRSR Sbjct: 901 AMNRIGGKSNTGEGGEDVNRYRNELKGIPIKQGATLASEIGKEHIEVDIPLLEGDSLRSR 960 Query: 954 IKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGGQLPGHKVTDYIATLRVSVPGVGLI 1013 IKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGGQLPGHKV++YIA LRVSVPGVGLI Sbjct: 961 IKQVASGRFGVSAEYLISADQIQIKMAQGAKPGEGGQLPGHKVSEYIAKLRVSVPGVGLI 1020 Query: 1014 SPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAKSDHVVIAGH 1073 SPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAKSDHVVIAGH Sbjct: 1021 SPPPHHDIYSIEDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAKSDHVVIAGH 1080 Query: 1074 DGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGALLG 1133 DGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGA+LG Sbjct: 1081 DGGTGASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGAMLG 1140 Query: 1134 ADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEHVVNFFFFIAEEV 1193 ADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEHVVNFFFFIAEE Sbjct: 1141 ADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEHVVNFFFFIAEEA 1200 Query: 1194 RQIMAQLGIRTFNELIGRSDLLDRTKAIQHWKAQGLDFSKIFYQPVSATPYFHTENQDHG 1253 RQIMAQLGIR F+ELIGR+DLLDR+KAI HWKA+GLDFS+IF+QP S P +HT++QDHG Sbjct: 1201 RQIMAQLGIRKFDELIGRADLLDRSKAIAHWKAKGLDFSRIFHQPESKLPVYHTDHQDHG 1260 Query: 1254 LDKALDHKLIAQARIALEKGEKVSFISPIRNLNRTVGAMLSGEVAKRYGNEGLPDDTIHI 1313 LDKALDHKLIAQA+ ALEKGEKVSFISPI+NLNRTVGAMLSGEVAKRYG+EGLPDDTIHI Sbjct: 1261 LDKALDHKLIAQAKTALEKGEKVSFISPIKNLNRTVGAMLSGEVAKRYGDEGLPDDTIHI 1320 Query: 1314 QLQGTAGQSAGAFLAHGVTLDLVGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNMISGNT 1373 QLQGTAGQSAGAFLA GVTLDLVGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNMISGNT Sbjct: 1321 QLQGTAGQSAGAFLAKGVTLDLVGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNMISGNT 1380 Query: 1374 VLYGATSGEAFINGVVGERFAVRNSGAIAVVEGTGDHGCEYMTGGSVVVLGATGRNFAAG 1433 VLYGA SGEAFINGV GERFAVRNSGA AVVEGTGDHGCEYMTGG+VVVLG TGRNFAAG Sbjct: 1381 VLYGAISGEAFINGVAGERFAVRNSGATAVVEGTGDHGCEYMTGGTVVVLGNTGRNFAAG 1440 Query: 1434 MSGGIAYVYDEAGDFAGKCNLAMVALDKVLSDTEQEASTDRAIWHSLQRGGDGQTDEVIL 1493 MSGGIAYVYD GDFAGKCN +MV+L+KVLSDTEQE + R IWHSLQRGG+ QTDE IL Sbjct: 1441 MSGGIAYVYDPEGDFAGKCNTSMVSLEKVLSDTEQEQTLGRDIWHSLQRGGERQTDEAIL 1500 Query: 1494 RGLIERHFKYTGSTRARARARXSTRARYLLDNWAASRGKFVKVFPTEYKRALGELHAAKA 1553 RGLIERHFKYTG STRARYLLDNWAASR KFVKVFPTEYKRAL EL AA Sbjct: 1501 RGLIERHFKYTG----------STRARYLLDNWAASRAKFVKVFPTEYKRALAELAAAAQ 1550 Query: 1554 APKDKVPA 1561 K+KV A Sbjct: 1551 TKKEKVAA 1558 >gi|300312905|ref|YP_003776997.1| glutamate synthase [Herbaspirillum seropedicae SmR1] Length = 531 Score = 132 bits (333), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 104/330 (31%), Positives = 144/330 (43%), Gaps = 56/330 (16%) Query: 858 ISIDEVEPAKEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDVNRYRNE 917 + D +P V F AMS GS+S A L + +TGEG V+ Y E Sbjct: 142 VGADRAQPYSMSV--FNVSAMSFGSLSANAIRALNQGAKKGNFAHDTGEG--SVSEYHRE 197 Query: 918 LKGIPIKQGATLASEIGKDVIEVDIPLQAGDSLRSRIKQVASGRFGV-------SAEYLI 970 G + Q+ASG FG + E Sbjct: 198 FGG-------------------------------DLVWQIASGYFGCRNADGSFNDEKFT 226 Query: 971 SADQ------IQIKMAQGAKPGEGGQLPGHKVTDYIATLRVSVPGVGLISPPPHHDIYSI 1024 + Q I++K++QGAKPG GG LP K+T IA R GV ISP H + Sbjct: 227 AQAQSPQIKMIEVKLSQGAKPGHGGVLPAAKITPEIAATRGIPMGVDCISPAVHSSFSTP 286 Query: 1025 EDLAQLIHDLKNVNPRASISVKLVSEVGVGTVAAGVAKAK------SDHVVIAGHDGGTG 1078 L + I L+ ++ + +KL VG G+AKA D + + G +GGTG Sbjct: 287 IGLLEFIEKLRGLSGGKPVGIKLC--VGHPWEFFGIAKAMLKTDIVPDFITVDGSEGGTG 344 Query: 1079 ASPLSSIKHAGSPWELGLAETQQTLVLNGLRNRIRVQADGQMKTGRDVVIGALLGADEFG 1138 A+PL + H G P + GL TLV GLR+++++ A G++ T DV LGAD Sbjct: 345 AAPLEFVDHVGMPLQEGLVMVHNTLVGIGLRDKVKIGASGKIITAFDVARTLALGADWCN 404 Query: 1139 FATAPLVVEGCIMMRKCHLNTCPVGVATQD 1168 A + GCI + CH + CP GVATQ+ Sbjct: 405 SARGFMFALGCIQSQSCHTDRCPTGVATQN 434 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,950,304 Number of extensions: 219055 Number of successful extensions: 639 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 632 Number of HSP's successfully gapped: 2 Length of query: 1561 Length of database: 1,619,870 Length adjustment: 98 Effective length of query: 1463 Effective length of database: 1,155,840 Effective search space: 1690993920 Effective search space used: 1690993920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 137 (57.4 bits)