BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0009.p (502 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310389|ref|YP_003774481.1| cyclopropane-fatty-acyl-phospho... 283 2e-77 gi|300310388|ref|YP_003774480.1| hypothetical protein Hsero_1055... 137 1e-33 gi|300310016|ref|YP_003774108.1| cyclopropane-fatty-acyl-phospho... 91 2e-19 >gi|300310389|ref|YP_003774481.1| cyclopropane-fatty-acyl-phospholipid synthase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 283 bits (725), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 152/266 (57%), Positives = 183/266 (68%), Gaps = 8/266 (3%) Query: 30 SAPPATRLFLRLLNCFSHGHLQLTTPHGNTLTFGDLHQPPSAHMVIRDWRACARMLQSGD 89 + P + RLFL +L SHGHL+L TPHG TLTFGDL QPPSA + I DWRAC +L++GD Sbjct: 14 AGPISARLFLAMLERLSHGHLELITPHGTTLTFGDLRQPPSAQLRILDWRACGAILRAGD 73 Query: 90 IGFAESYAAGWIDTPDLSALLRLCLRNQAELERMMFGGKLAGLWYRLRHLMRANTRAGSR 149 IGF E+Y GW+DTPDL+ALLRL +RN+ L + GG LA WYRLRHL+R+NTR+GSR Sbjct: 74 IGFGEAYGHGWVDTPDLAALLRLAMRNRQVLSSAVEGGWLASWWYRLRHLLRSNTRSGSR 133 Query: 150 KNIHAHYDIGNAFYHQWLDDSWTYSSAIFDGDHGQSLEAAQARKYQRIIDQLGLQAGDHV 209 +NIHAHYD+GN FY WLD + +YSSAIF GQSLE AQA KYQRI+DQLGL+AGD V Sbjct: 134 RNIHAHYDLGNDFYRLWLDPTMSYSSAIFSAP-GQSLEQAQAAKYQRIVDQLGLRAGDRV 192 Query: 210 LEIGCGWGGFAEHAASRGIR--DVRLNLARLSDANNAWFGVDRLRLMSLRTRDYGPRDGS 267 LEIGCGWGGFAEHAA GIR V ++ A+L+ A L L DY Sbjct: 193 LEIGCGWGGFAEHAARLGIRVHGVTISPAQLAYAQERMRTQQLDHLAHLELCDY-----R 247 Query: 268 DLDGWMRAQLADAGLPCDGEIWLQTF 293 DLDG A ++ GE + QT+ Sbjct: 248 DLDGQYDAIVSIEMFEAVGERFWQTY 273 Score = 256 bits (655), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 122/169 (72%), Positives = 141/169 (83%) Query: 334 LDQLARLELCDYRDLKGEYDAVVSIEMFEAVGERFWQQYFDTVSARLKKGGRAMIQSITI 393 LD LA LELCDYRDL G+YDA+VSIEMFEAVGERFWQ YFDTV ARLK GG+A+IQSITI Sbjct: 235 LDHLAHLELCDYRDLDGQYDAIVSIEMFEAVGERFWQTYFDTVCARLKAGGQALIQSITI 294 Query: 394 AEQYFERYRSTSDFIRQYIFPGGMLPSPERFCAKARQAGLQTLDQYGFGRDYAETLRRWN 453 E FERYR ++DFIR++IFPGGMLPS ERF A++AGL T +++ FGRDYAETLRRW+ Sbjct: 295 EEAVFERYRDSADFIREFIFPGGMLPSHERFSRCAQRAGLATRERFHFGRDYAETLRRWH 354 Query: 454 ASFLHARDAIRKQGFDERFMRLWQLYYAYCEVGFDEERTDVIQFMLQKN 502 SFL R+ I QGFDERF RLWQLYY +CEVGFDE +TDV+QF+L K Sbjct: 355 DSFLAQRETIAAQGFDERFRRLWQLYYVFCEVGFDEGQTDVVQFLLHKE 403 >gi|300310388|ref|YP_003774480.1| hypothetical protein Hsero_1055 [Herbaspirillum seropedicae SmR1] Length = 283 Score = 137 bits (346), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 64/84 (76%), Positives = 71/84 (84%) Query: 231 VRLNLARLSDANNAWFGVDRLRLMSLRTRDYGPRDGSDLDGWMRAQLADAGLPCDGEIWL 290 VRL+LARL++A NAWFGVDR RLM +RTRDYGPR+GSDL W+R L AGLP DG IWL Sbjct: 38 VRLDLARLAEAGNAWFGVDRRRLMCVRTRDYGPRNGSDLMQWVRGHLQQAGLPDDGAIWL 97 Query: 291 QTFPRLFGFVFNPVSFWLCHDSAG 314 QTFPRLFGFVFNPVSF+LCHD G Sbjct: 98 QTFPRLFGFVFNPVSFFLCHDREG 121 >gi|300310016|ref|YP_003774108.1| cyclopropane-fatty-acyl-phospholipid synthase [Herbaspirillum seropedicae SmR1] Length = 407 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 59/180 (32%), Positives = 86/180 (47%), Gaps = 3/180 (1%) Query: 323 LARLELXAARGLDQLARLELCDYRDLKGEYDAVVSIEMFEAVGERFWQQYFDTVSARLKK 382 LAR + AA GL + L DYRD+ G +D + S+ MFE VG + YF + L Sbjct: 212 LARERVEAA-GLSDRIEIRLQDYRDVTGSFDRITSVGMFEHVGIKNLPSYFAQIRKLLAD 270 Query: 383 GGRAMIQSITIAE-QYFERYRSTSDFIRQYIFPGGMLPSPERFCAKARQAGLQTLDQYGF 441 GG AM IT + + E +FI +Y+FP G LP ++ GL+ D Sbjct: 271 GGMAMNHGITSTDIENGESPYGAGEFIDKYVFPAGELPHISTVLKTMQEGGLEAFDVENL 330 Query: 442 GRDYAETLRRWNASFLHARDAIRKQGFDERFMRLWQLYYAYCEVGFDEERTDVIQFMLQK 501 R YA T W +F +AI + DE+ R+W++Y A GF+ + + Q + K Sbjct: 331 RRHYARTCGIWADNFEANTEAIHRVT-DEKHYRIWRVYLAGSAYGFERDWISLYQVVCTK 389 Score = 69.7 bits (169), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 54/191 (28%), Positives = 88/191 (46%), Gaps = 14/191 (7%) Query: 50 LQLTTPHGNTLTFGDLHQPPSAHMVIRDWRACARMLQSGDIGFAESYAAGWIDTP-DLSA 108 L+L +G FG P + + A +L E+Y G I+ + + Sbjct: 28 LRLVLWNGQQYDFGS--GTPEVTLKVPHASAITSLLTPSLANLGEAYVEGKIEVEGKVKS 85 Query: 109 LLRLCLRNQAELERMMFGGKLAGLWYRLRHLMRANTRAGSRKNIHAHYDIGNAFYHQWLD 168 ++++ N + G+L + + H TR + I HYD+ N FY +LD Sbjct: 86 IIQVA--NSLASNTLKAEGRLGRMVRSVTH-----TRKRDAEAIQYHYDVSNDFYKLFLD 138 Query: 169 DSWTYSSAIFD-GDHGQSLEAAQARKYQRIIDQLGLQAGDHVLEIGCGWGGFAEHAASR- 226 + YS A F+ GD + L AQ +K I++++ L+ G +L+IGCGWG AA + Sbjct: 139 EHMVYSCAYFENGD--EDLATAQIKKIDHILEKIQLRPGQTLLDIGCGWGALVLRAAQKY 196 Query: 227 GIRDVRLNLAR 237 G R V + L+R Sbjct: 197 GARCVGVTLSR 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,682,839 Number of extensions: 72865 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 271 Number of HSP's successfully gapped: 5 Length of query: 502 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 413 Effective length of database: 1,198,455 Effective search space: 494961915 Effective search space used: 494961915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)