BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0214.p (232 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310505|ref|YP_003774597.1| V-type H(+)-translocating pyrop... 230 6e-62 >gi|300310505|ref|YP_003774597.1| V-type H(+)-translocating pyrophosphatase [Herbaspirillum seropedicae SmR1] Length = 682 Score = 230 bits (587), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 112/128 (87%), Positives = 123/128 (96%) Query: 15 MAGALWFAVACGVVAVIYGLISRSWILKQDPGNQRMQDIATAIQQGAAAYLARQYRTIAL 74 MA ALWFA+ACG+VAVIYGL+SR+WILKQDPGN RMQDIA AIQQGA+AYLARQYRTIA+ Sbjct: 3 MALALWFAIACGIVAVIYGLVSRNWILKQDPGNPRMQDIALAIQQGASAYLARQYRTIAM 62 Query: 75 VGVVLFILIALIPGLGWITAVGFLIGAVLSGACGFIGMNVSVRANVRTAQAATKGMNEAL 134 VGVVL ILIALIPGLGW+TA+GFL+GAVLSGACGFIGMNVSVRANVRTAQAAT+GMN+AL Sbjct: 63 VGVVLLILIALIPGLGWLTAIGFLLGAVLSGACGFIGMNVSVRANVRTAQAATQGMNQAL 122 Query: 135 NVAFRGGA 142 VAFRGGA Sbjct: 123 AVAFRGGA 130 Score = 127 bits (319), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 65/79 (82%), Positives = 72/79 (91%), Gaps = 4/79 (5%) Query: 144 PITDNAGGIAEMSGMPESVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHAL 203 PITDNAGGIAEMSG+PESVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHAL Sbjct: 428 PITDNAGGIAEMSGLPESVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHAL 487 Query: 204 DAVGKYPRVAATTSATAPA 222 ++VGK +A+ ++PA Sbjct: 488 ESVGK----SASFDLSSPA 502 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 754,716 Number of extensions: 30840 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 2 Length of query: 232 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 150 Effective length of database: 1,231,600 Effective search space: 184740000 Effective search space used: 184740000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)