BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0208.p
         (384 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310511|ref|YP_003774603.1| tRNA and rRNA cytosine-C5-methy...   630   0.0  
gi|300309418|ref|YP_003773510.1| RNA methyltransferase [Herbaspi...    79   6e-16

>gi|300310511|ref|YP_003774603.1| tRNA and rRNA
           cytosine-C5-methylase [Herbaspirillum seropedicae SmR1]
          Length = 419

 Score =  630 bits (1626), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 306/403 (75%), Positives = 341/403 (84%), Gaps = 20/403 (4%)

Query: 1   LREILRFAGPADVTLSRYFRENPKLGGRERGVIAEAVYGLLRNKSVFTNFAESGVGPMMR 60
           LRE+LRF GPADVTLSRYFR+NP+LG RERGVIAEAVYGLLRNK+V TNFAESG GPMMR
Sbjct: 15  LREVLRFTGPADVTLSRYFRDNPRLGSRERGVIAEAVYGLLRNKTVLTNFAESGSGPMMR 74

Query: 61  RMALLGLVDTVSIESIAGLSPEEVEWLTRTAQIDRNKLPLAMRANMPPWLLDKLIARDGE 120
           R+ALLGL D V  ESIAGL   E +WL R AQIDR +L   MR+N+PPWL DKL+AR GE
Sbjct: 75  RLALLGLADAVGAESIAGLQEGEADWLERVAQIDRTQLAAQMRSNLPPWLWDKLVARMGE 134

Query: 121 AAAMEMAEALNQPAPLDLRVNAIKANREDVMAALAAAPILCEATPYSPLGLRVIKKPALQ 180
           AA +E+AEA+N+PAPLDLRVNA+KA+R+ V+A LA AP+ CE TPYSPLGLRV+KKPALQ
Sbjct: 135 AATLELAEAMNRPAPLDLRVNALKADRDTVIAELAKAPVACEPTPYSPLGLRVLKKPALQ 194

Query: 181 NLPLFKDGAIEVQDEGSQLLAQIVGVKRGEMVVDFCA--------------------XFD 220
           NLPLFKDGAIEVQDEGSQLLAQIVG KRGEMVVDFCA                     FD
Sbjct: 195 NLPLFKDGAIEVQDEGSQLLAQIVGAKRGEMVVDFCAGAGGKTLALGALMRNTGRLYAFD 254

Query: 221 ISEKRLAKLKPRLARSGLSNVHPVMIAHENDAKVKRLAGKIDRVLVDAPCSGLGTLRRNP 280
           +SEKRLAKLKPRLARSGLSNVHPV+IAHENDAKVKRLAGK+DRVLVDAPCSGLGTLRRNP
Sbjct: 255 VSEKRLAKLKPRLARSGLSNVHPVVIAHENDAKVKRLAGKLDRVLVDAPCSGLGTLRRNP 314

Query: 281 DMKWRQTVETVAEMNVKQISILTSASRLVKAGGRLVYGTCSFLNEENEDIVAQFLQANPD 340
           DMKWRQTVET+AEM  KQ SIL SA+RLV+AGGRLVYGTCSFL EEN++++A FLQ++PD
Sbjct: 315 DMKWRQTVETLAEMRAKQSSILASAARLVRAGGRLVYGTCSFLEEENDEVIADFLQSHPD 374

Query: 341 FTLVPMNTVLAEQKIDLDTGDYLKMLPHVHHTDGFFAAVLERK 383
           F L PM  VLAEQKI L+ GDYLK+LPH H TDGFFAAVLER+
Sbjct: 375 FILRPMGEVLAEQKIALEMGDYLKLLPHQHQTDGFFAAVLERR 417


>gi|300309418|ref|YP_003773510.1| RNA methyltransferase
           [Herbaspirillum seropedicae SmR1]
          Length = 424

 Score = 79.0 bits (193), Expect = 6e-16,   Method: Compositional matrix adjust.
 Identities = 79/300 (26%), Positives = 126/300 (42%), Gaps = 45/300 (15%)

Query: 105 NMPPWLLDKLIARDGEAAAMEMAEAL----NQPAPLDLRVNAIKANREDVMAALAAAPIL 160
           N P W +D+L     +AA  E  +A+    NQ  PL LRVN  +      +  L  A + 
Sbjct: 145 NYPTWWIDRL-----KAAYPEQWQAILHAGNQAPPLTLRVNRRRTTVAQYLDTLQQAGM- 198

Query: 161 CEATPYSPLGLRVIKKPALQNLPLFKDGAIEVQDEGSQLLAQIVGVKRGEMVVDFCAX-- 218
             A    P  +R+ +   +Q LP F +G + VQD  +QL A ++ V  G  V+D CA   
Sbjct: 199 -AARQVGPAAVRLHRPVPVQQLPGFAEGVVSVQDAAAQLAAPLLDVADGMRVLDACAAPG 257

Query: 219 ----------------FDISEKRLAKLKPRLARSGLSNVHPVMIAHENDAKVKRLAGKID 262
                            D   +RL ++   L R  L        A  +D    +     D
Sbjct: 258 GKTGHLLELADLDLQALDHDTRRLQRIAENLDRLQLKARLTTGDARADDWWDGQ---PFD 314

Query: 263 RVLVDAPCSGLGTLRRNPDMKWRQTVETVAEMNVKQISILTSASRLVKAGGRLVYGTCSF 322
           R+L D PC+  G +RR+PD++W +     A++      IL    ++++  G+L+  TCS 
Sbjct: 315 RILADVPCTASGIVRRHPDIRWLRRKTDTAQLATLSTQILDKLWQMLRPDGKLLLVTCSL 374

Query: 323 LNEENEDIVAQFLQANPDFTLVPMNTVLAEQKIDLDTGDYLKMLPHVHHTDGFFAAVLER 382
             +E+E     F Q +    L     +L       +T D+          DG F A+ ++
Sbjct: 375 WPQESEQQAVAFAQKHNAIRLPAPGQLLPTAS---ETADH----------DGLFYALFQK 421


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,112,596
Number of extensions: 42867
Number of successful extensions: 148
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 146
Number of HSP's successfully gapped: 2
Length of query: 384
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 297
Effective length of database: 1,207,925
Effective search space: 358753725
Effective search space used: 358753725
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)