BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0197.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 546 e-157 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 546 e-157 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 224 9e-60 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 161 6e-41 gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC t... 154 9e-39 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 546 bits (1407), Expect = e-157, Method: Compositional matrix adjust. Identities = 283/309 (91%), Positives = 302/309 (97%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDVLMIGAMAGLSILKLIQHV 60 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGD+LM+GAM GLS+LK++Q V Sbjct: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 Query: 61 APDLPGIVKLIIAIIGAIPVCVIVSMIIERVAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 AP LPGIV+L+IAI+GAIPVC++VS++IER+AYRPLRNAPRLAPLITAIGVSILLQTLAM Sbjct: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 Query: 121 MIWGRSPLPFPQIMPSDPVHIAGALISPTQIMLLLLALAAMIGLVLIVEKTKMGRAMRAT 180 MIWGRSPLPFPQ+MPSDPVHIAGALISPTQIMLL LA+ AM+GLVLIVEKTKMGRAMRAT Sbjct: 121 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 Query: 181 AENPRIAGLMGVDSNRVIVVTFIIGAALAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 AENPRIAGLMGVD+N+VIVVTF IGA LAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV Sbjct: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 Query: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGDFLGSNYQDIFAFIVLIIVLTLRPSGI 300 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTG+FLGSNYQDIFAFIVLIIVLTLRPSGI Sbjct: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI 300 Query: 301 MGERVADRA 309 MGERVADRA Sbjct: 301 MGERVADRA 309 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 546 bits (1407), Expect = e-157, Method: Compositional matrix adjust. Identities = 283/309 (91%), Positives = 302/309 (97%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDVLMIGAMAGLSILKLIQHV 60 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGD+LM+GAM GLS+LK++Q V Sbjct: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQV 60 Query: 61 APDLPGIVKLIIAIIGAIPVCVIVSMIIERVAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 AP LPGIV+L+IAI+GAIPVC++VS++IER+AYRPLRNAPRLAPLITAIGVSILLQTLAM Sbjct: 61 APGLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAM 120 Query: 121 MIWGRSPLPFPQIMPSDPVHIAGALISPTQIMLLLLALAAMIGLVLIVEKTKMGRAMRAT 180 MIWGRSPLPFPQ+MPSDPVHIAGALISPTQIMLL LA+ AM+GLVLIVEKTKMGRAMRAT Sbjct: 121 MIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 Query: 181 AENPRIAGLMGVDSNRVIVVTFIIGAALAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 AENPRIAGLMGVD+N+VIVVTF IGA LAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV Sbjct: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 Query: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGDFLGSNYQDIFAFIVLIIVLTLRPSGI 300 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTG+FLGSNYQDIFAFIVLIIVLTLRPSGI Sbjct: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLRPSGI 300 Query: 301 MGERVADRA 309 MGERVADRA Sbjct: 301 MGERVADRA 309 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 224 bits (570), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 137/314 (43%), Positives = 201/314 (64%), Gaps = 27/314 (8%) Query: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDVLMIGAMAGLSILKLIQHVAPDLP 65 QQ++NGL LG++YALIA+GYTMVYG++ +INFAHG++ MI + GL L I V P Sbjct: 10 QQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAI-GVQSGYP 68 Query: 66 GIVKLIIAIIGAIPVCVIVSMIIERVAYRPLRNAPRLAPLITAIGVSILLQTLAMMIWGR 125 + L A+I ++ V + +ERVAYRPLR PRL PLI+AIG+SI LQ + G Sbjct: 69 LPLLLGGALIVSVLVTGLYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQIGQGA 128 Query: 126 SPLPFPQIMPSDPVHIAGAL-----------ISPTQIMLLLLALAAMIGLVLIVEKTKMG 174 + S PV I+GAL I ++++++ + L M+ L L + +++MG Sbjct: 129 RDM-------SVPVLISGALEFQMGSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSRMG 181 Query: 175 RAMRATAENPRIAGLMGVDSNRVIVVTFIIGAALAAIAGVMWAANYSTAQFAMGFVPGLK 234 RA RA AE+ +A L+G+D+N+VI TF++GA LAA+ GV+ A +GF+ G+K Sbjct: 182 RACRACAEDMGMANLLGIDTNKVISFTFVLGAMLAAVGGVLIALTIGKLNPYIGFIAGIK 241 Query: 235 AFSAAVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGDFLGSNYQDIFAFIVLIIVLT 294 AF+AAVLGGIG+I GAMLGG+LLGL E+ AGY L S Y+D+ +F +L+++L Sbjct: 242 AFTAAVLGGIGSIPGAMLGGVLLGLAETFAAGY--------LPSEYKDVVSFGLLVLILL 293 Query: 295 LRPSGIMGERVADR 308 RP+G++G+ ++ Sbjct: 294 FRPTGLLGKPDVEK 307 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 161 bits (407), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 110/307 (35%), Positives = 187/307 (60%), Gaps = 20/307 (6%) Query: 6 QQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDVLMIGAMAGLSILKLIQHVAPDLP 65 QQ+IN L LGS+YAL ALG+T+V+GVL +IN +HG + M+G+ A L L++ +A LP Sbjct: 4 QQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAAL---LLVEQMA--LP 58 Query: 66 GIVKLIIAIIGAIPVCVIVSMIIERVAYRPLR--NAPRLAPLITAIGVSILLQTLAMMIW 123 V L++A++ ++ +II+ + +PLR NAP L P+I IGV+I++ ++ ++ Sbjct: 59 LWVALLLAMVA----TGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLF 114 Query: 124 GRSPLPFPQ-IMPSDPVHIAGALISPTQIMLLLLALAAMIGLVLIVEKTKMGRAMRATAE 182 G FPQ +P + V + I+ Q+ ++++A M+ L+ ++ KT++GRA+RA AE Sbjct: 115 GAENKRFPQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAE 174 Query: 183 NPRIAGLMGVDSNRVIVVTFIIGAALAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAVLG 242 +P+ A L+G++ + ++T AAL AGV+ +++ MG K + +LG Sbjct: 175 SPKAAYLLGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILG 234 Query: 243 GIGNIYGAMLGGILLGLIESLGAGYIGDLTGDFLGSNYQDIFAFIVLIIVLTLRPSGIMG 302 G+G+I GAM+GG+ LG E L YI S+++D AF +L ++L ++PSG+ G Sbjct: 235 GMGDIRGAMIGGLFLGFAEVLTVAYI--------SSDFRDAVAFGLLFLILLVKPSGMFG 286 Query: 303 ERVADRA 309 + + +A Sbjct: 287 KVLERKA 293 >gi|300311114|ref|YP_003775206.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 296 Score = 154 bits (389), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 100/310 (32%), Positives = 182/310 (58%), Gaps = 17/310 (5%) Query: 1 MDIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDVLMIGAMAGLSILKLIQHV 60 MDI +Q + +G+ LG +YA+IA GY + + +NF G+ LM+GA+ GLS++ I H Sbjct: 1 MDILLQLVFSGIALGMIYAVIAFGYQLTFATSGTLNFGQGEALMLGALVGLSVVGNI-HG 59 Query: 61 APDLPGIVKL-IIAIIGAIPVCVIVSMIIERVAYRP-LRNAPRLAPLITAIGVSILLQTL 118 P L + + I+ + GA+ M +E + RP ++ +++ I ++I+ + + Sbjct: 60 GPYLNYWLMIPIVLVFGALQ-----GMFVEWIGVRPAIKIKSEFGWIMSTIALAIIFKNV 114 Query: 119 AMMIWGRSPLPFPQIMPSDPVHIAGALISPTQIMLLLLALAAMIGLVLIVEKTKMGRAMR 178 A IWG+ LPFP + P I GA + P Q+++++ AL M + L K+ G+A+ Sbjct: 115 AENIWGKDDLPFPSPISGAPFQIFGANVQPMQVLVVVGALLIMAAVELFNRKSIYGKAVV 174 Query: 179 ATAENPRIAGLMGVDSNRVIVVTFIIGAALAAIAGVMWAANYSTAQFAMGFVPGLKAFSA 238 AT+ + AGLMG++++ VI ++ + +A AA AGV+ A + MG GLKAF+ Sbjct: 175 ATSNDRDAAGLMGINTSMVITFSYALSSATAAFAGVL-VAPLTLTGATMGAALGLKAFAV 233 Query: 239 AVLGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGDFLGSNYQDIFAFIVLIIVLTLRPS 298 A++GG+ + GA++GG++LG+ E+ LTG ++ + Y+++ ++L++VL ++P+ Sbjct: 234 AIIGGLTSGMGAIVGGLILGVAET--------LTGFYISTGYKEVPGLVLLLLVLAVKPA 285 Query: 299 GIMGERVADR 308 G+ G+ + Sbjct: 286 GLFGKTAIKK 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.145 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 717,608 Number of extensions: 22572 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 34 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)