BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0186.p (886 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309523|ref|YP_003773615.1| bifunctional proline dehydrogen... 1182 0.0 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 85 3e-17 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 72 2e-13 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 69 1e-12 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 68 3e-12 >gi|300309523|ref|YP_003773615.1| bifunctional proline dehydrogenase/proline oxidase delta-1-pyrroline-5-carboxylate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 1230 Score = 1182 bits (3059), Expect = 0.0, Method: Compositional matrix adjust. Identities = 597/733 (81%), Positives = 640/733 (87%), Gaps = 2/733 (0%) Query: 18 FAPAFSVFQQEVMHAPTPLRAAITAVYRRDERGAVQWLLAQVQSNATWTEATQKLARKLV 77 A F FQ E++ P+PLRAAITA YRRDER AVQWLL QVQ W +ATQ+LARKLV Sbjct: 9 VAAPFGGFQAELLPTPSPLRAAITAAYRRDEREAVQWLLQQVQEEQPWKDATQQLARKLV 68 Query: 78 QAVRTKRTRSSGVDALMHEFSLSSEEGVALMCLAEALLRIPDHQTADRLIADKISKGDWR 137 Q VR KRTRSSGVDALMHEFSLSSEEGVALMCLAEALLRIPD QTADRLIADKISKGDWR Sbjct: 69 QQVREKRTRSSGVDALMHEFSLSSEEGVALMCLAEALLRIPDRQTADRLIADKISKGDWR 128 Query: 138 KHLGESPSLFVNAATWGLLITGKLVGTSSESGLASALTRLINKGGEPLIRKGVDLAMRML 197 KHLGESPSLFVNAATWGLLITGKLV TSSESGL A+TRLI KGGEPLIRKGVDLAMRML Sbjct: 129 KHLGESPSLFVNAATWGLLITGKLVSTSSESGLTQAITRLIGKGGEPLIRKGVDLAMRML 188 Query: 198 GNQFVTGQTIAEALANSRDNEKRGYRYSYDMLGEAALTGHDADFYYKSYEDAIHAIGRAS 257 GNQFVTGQTI EAL NSR+NEKRGYRYSYDMLGEAALT HDAD YY+SYE AIHAIGRAS Sbjct: 189 GNQFVTGQTIEEALDNSRENEKRGYRYSYDMLGEAALTMHDADAYYQSYESAIHAIGRAS 248 Query: 258 NGRGIKDGPGISVKLSALHPRYSRAQHARVMNELLPRLKQLLVLAKSYNIGLNIDAEEAD 317 NGRGIKDGPGISVKLSALHPRYSRAQHARVM+ELLPRLKQLL+LAK Y+IGLNIDAEEAD Sbjct: 249 NGRGIKDGPGISVKLSALHPRYSRAQHARVMSELLPRLKQLLLLAKQYDIGLNIDAEEAD 308 Query: 318 RLELSLDLMEALAFDRDLDGFEGIGFVVQAYQKRCPFVIDYLADLARRSGRKLMVRLVKG 377 RLELSLD+ME L D DL GF+G+GFVVQ YQKRCPFVIDYL DLARR+GR+LM+RLVKG Sbjct: 309 RLELSLDMMEVLVADPDLAGFDGLGFVVQGYQKRCPFVIDYLVDLARRNGRRLMIRLVKG 368 Query: 378 AYWDSEIKRAQVDGLSGYPVYTRKVHTDLSYLACAQKLLAATDVIYPQFATHNAHTLSAI 437 AYWDSEIKRAQVDGL GYPVYTRKVHTDLSYL CAQKLLAATDVIYPQFATHNAHTL+AI Sbjct: 369 AYWDSEIKRAQVDGLEGYPVYTRKVHTDLSYLTCAQKLLAATDVIYPQFATHNAHTLAAI 428 Query: 438 YTWAQQHKIDDYEFQCLHGMGETLYDQVVGADQLGKACRIYAPVGSHQTLLAYLVRRLLE 497 Y WA+QH+ID+YEFQCLHGMGETLYDQVVG D LGKACR+YAPVGSHQTLLAYLVRRLLE Sbjct: 429 YHWARQHQIDNYEFQCLHGMGETLYDQVVGPDNLGKACRVYAPVGSHQTLLAYLVRRLLE 488 Query: 498 NGANSSFVNQIVDEAVAIDTLITDPIVAVQKSGGEPHPQIALPRNLYGEERKNSAGVDLS 557 NGANSSFVNQIVDEAV +D L+ DPI V+ GG PHP IA+P LYGEERKNSAG+DLS Sbjct: 489 NGANSSFVNQIVDEAVPLDRLVGDPIETVRAQGGLPHPAIAVPHRLYGEERKNSAGIDLS 548 Query: 558 NEDTLRGLEHAFAQLSARQWQAAPLLHDGAVSIGETQA--ITNPADRRDLVGQVIEASHD 615 NED L+ L F ++ RQWQAAPLL + A + NPAD R++VGQV EA+ Sbjct: 549 NEDRLQQLGQLFISMADRQWQAAPLLAADTAAQSAQAAQLVRNPADLREVVGQVSEATVA 608 Query: 616 DVESALHAATEYAVQWQAVSSGDRAQALERAADLFETHQAELMALAVREAGKSLPNAIAE 675 DV++AL AAT+YA QWQ+ + +RA LERAADL E H AELMALAVREAGKSLPNAIAE Sbjct: 609 DVDTALRAATDYAPQWQSTPATERAAMLERAADLLEEHIAELMALAVREAGKSLPNAIAE 668 Query: 676 VREAVDFLRYYATQSRHDGNALAWGPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAE 735 VREAVDFLRYYA SRHDGN LAWGPVVCISPWNFPLAIFIGEVSA+LAAGNVVLAKPAE Sbjct: 669 VREAVDFLRYYAIASRHDGNVLAWGPVVCISPWNFPLAIFIGEVSAALAAGNVVLAKPAE 728 Query: 736 QTPLIAHRAVXFI 748 QT LIAHRAV + Sbjct: 729 QTALIAHRAVQLL 741 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 84.7 bits (208), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 59/183 (32%), Positives = 91/183 (49%), Gaps = 18/183 (9%) Query: 591 GETQAITNPADRRDLVGQVIEASHDDVESALHAATEYAVQWQAVSSGDRAQALERAADLF 650 G T + NPA +++G++ A D++ AL AA++ W+ +S+ +R++ + RAADL Sbjct: 19 GRTIDVINPATE-EVIGKIAHADRADLDRALEAASKGFETWRNMSAFERSKIMRRAADLL 77 Query: 651 ETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQSRHDGNALA------------ 698 E+ L E GK L A E A D + ++A ++R L Sbjct: 78 RERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPARVPGAYQMVI 137 Query: 699 ---WGPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQTPLIAHRAVXFIAARGAAI 755 GPV +PWNFPL + ++SA+LAAG ++ K E+TP A A A A + Sbjct: 138 KEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP--ASPAELIRAYADAGV 195 Query: 756 PAG 758 PAG Sbjct: 196 PAG 198 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 72.0 bits (175), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 52/157 (33%), Positives = 76/157 (48%), Gaps = 15/157 (9%) Query: 608 QVIEASHDDVESALHAATEYAVQWQAVSSGDRAQALERAADLFETHQAELMALAVREAGK 667 +V +S +DVE AL AA W S +RA L + AD E + L + GK Sbjct: 49 EVARSSAEDVELALDAAHAAKKSWGKTSPTERANMLLKIADRMEANLELLATAETLDNGK 108 Query: 668 SLPNAIA-EVREAVDFLRYYATQSRH--------DGNALAW------GPVVCISPWNFPL 712 + +A ++ A+D RY+A R D + A+ G V I PWNFP+ Sbjct: 109 PIRETMAADIPLAIDHFRYFAAAVRTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPI 168 Query: 713 AIFIGEVSASLAAGNVVLAKPAEQTPLIAHRAVXFIA 749 + + +++ +LAAGN V+ KPAEQTP + IA Sbjct: 169 LMAVWKLAPALAAGNCVVLKPAEQTPASIMVLIELIA 205 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 69.3 bits (168), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 57/183 (31%), Positives = 87/183 (47%), Gaps = 18/183 (9%) Query: 591 GETQAITNPADRRDLVGQVIEASHDDVESALHAATEYAVQWQAVSSGDRAQALERAADLF 650 GE A+ NPA+ +L+G V + A+ AA W+ ++ +R+ L R +L Sbjct: 26 GERLAVHNPANG-ELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERSAILRRWYELM 84 Query: 651 ETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQS-RHDGNAL------------ 697 + +L + E GK L A E+ A F+ ++A + R G+ + Sbjct: 85 LANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVI 144 Query: 698 --AWGPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQTPLIAHRAVXFIAARGAAI 755 A G I+PWNFP A+ + +LAAG ++ KPAE TP A A+ +A R A I Sbjct: 145 KEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA-LALAVLAER-AGI 202 Query: 756 PAG 758 PAG Sbjct: 203 PAG 205 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 68.2 bits (165), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 46/135 (34%), Positives = 68/135 (50%), Gaps = 17/135 (12%) Query: 639 RAQALERAADLFETHQAELMALAVREAGKSLPNAIAEVREAVDFLRYYATQ-SRHDGNAL 697 RA ++R DL HQ +L L RE GK L A EV A ++ ++ + +R +G+ + Sbjct: 75 RAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDII 134 Query: 698 A--------------WGPVVCISPWNFPLAIFIGEVSASLAAGNVVLAKPAEQTPLIAHR 743 G V I+PWNFP A+ +++ +LAAG V+ KPAE TPL + Sbjct: 135 PAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLA 194 Query: 744 AVXFIAARGAAIPAG 758 V A+ A +P G Sbjct: 195 LVRL--AQEAGVPVG 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,741,108 Number of extensions: 117255 Number of successful extensions: 398 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 393 Number of HSP's successfully gapped: 11 Length of query: 886 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 792 Effective length of database: 1,174,780 Effective search space: 930425760 Effective search space used: 930425760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)