BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0175.p (127 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogen... 151 1e-38 gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogen... 91 3e-20 >gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 151 bits (382), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 78/120 (65%), Positives = 91/120 (75%), Gaps = 3/120 (2%) Query: 4 TSGAPRWRIXPGDLEQPGKLVRRYDFARFLAGFSERHSWLPPALAKRYARSYGSRAERLL 63 T+GAP + GDLEQPGK+V RYDF +L F RH WLP LA RYARSYGSR E+++ Sbjct: 385 TAGAP---LPGGDLEQPGKMVARYDFDAYLRKFQARHPWLPAELAHRYARSYGSRVEQII 441 Query: 64 KGAAALADLGEELAPGLYESEARYLRDVEWACSAEDILWRRSKLGLYCRPQHVERLQQWL 123 A +LA LGEEL+PGLYE+EARYL EWA SA+DILWRRSKLGL+C PQH RL+ WL Sbjct: 442 GQADSLAALGEELSPGLYEAEARYLVQHEWARSADDILWRRSKLGLHCGPQHRGRLEAWL 501 >gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 90.9 bits (224), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 52/112 (46%), Positives = 71/112 (63%), Gaps = 5/112 (4%) Query: 15 GDL--EQP-GKLVRRYDFARFLAGFSERHSWLPPALAKRYARSYGSRAERLLKGAAALAD 71 GDL QP + VR++D +++ G +++ WLP AL +RYAR+YGSR LL+G + + Sbjct: 400 GDLYGAQPSNRSVRQFD--QYVQGLQQQYPWLPAALLQRYARAYGSRTHTLLEGRQNVEE 457 Query: 72 LGEELAPGLYESEARYLRDVEWACSAEDILWRRSKLGLYCRPQHVERLQQWL 123 +GEE+ GLY +E YL EWA +A DILWRRSKLGL+ L WL Sbjct: 458 MGEEILDGLYAAEVDYLLRHEWASNAADILWRRSKLGLHFPKGSEAVLDAWL 509 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 383,421 Number of extensions: 11392 Number of successful extensions: 29 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 29 Number of HSP's successfully gapped: 2 Length of query: 127 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 52 Effective length of database: 1,264,745 Effective search space: 65766740 Effective search space used: 65766740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)