BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0167.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 863 0.0 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 140 3e-34 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 126 5e-30 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 114 2e-26 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 68 2e-12 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 863 bits (2230), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/493 (84%), Positives = 450/493 (91%) Query: 1 MAYLLALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQ 60 MAYLLALDQGTSSSRSIIFD++GAIVATAQQEF QIFPQPGWVEHDPMDLW+SQLQT ++ Sbjct: 1 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQK 60 Query: 61 VLAQAGIKAADLAGLGITNQRETTVVWDRATGKPVHNAIVWQDRRTEAICAELRERGLTD 120 VLAQ+GIKA++L LGITNQRETTVVW+RATGKPV+NA+VWQDRR E +CA LRERGL + Sbjct: 61 VLAQSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE 120 Query: 121 AIQAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTGGRLHVTD 180 AIQ KTGLVLDPYFSGTKL WILD V GLR RA RGEL FGTVD+WL+WNLTGGRLHV+D Sbjct: 121 AIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 Query: 181 VSNASRTMLWNIHTGNWDDELLEWLDVPRSMLPEVHPSAHLYGHTDAGHLGAAVVIGGIA 240 VSNASRTMLWNIHTG WD+ELL+WL +P +MLP+V PSAHLYG DA HLGAAV IGGIA Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEIDAQHLGAAVKIGGIA 240 Query: 241 GDQQSALFGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGERKQYALE 300 GDQQSALFGQAC +PGMAKNTYGTGCFMLLHTGDQCA S NGLISTAACQV E+KQYALE Sbjct: 241 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQYALE 300 Query: 301 GSVFIGGAVVQWLRDGLKAIKNSTDVEELAASAPDSGGVVFVPSFTGLGAPYWNPSAKGA 360 GSVFIGGAVVQWLRDGLKAIK STDVE LA S DSGGVVFVPSFTGLGAPYWNPSAKGA Sbjct: 301 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNPSAKGA 360 Query: 361 IVGLSRGSTVAHIARAALESIAFQSTALLTAMVRDAVSPITELRVDGGASANNLLLQFQA 420 IVGLSRGSTVAHIARAALESIAFQSTALL AMVRDAVSPITELRVDGGA+ANNLLLQFQA Sbjct: 361 IVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGGAAANNLLLQFQA 420 Query: 421 DLLGIPVIRPQVTETTALGAASLAGLATGVYRDMAELSEHWRMERTFLPTMSRDEAQTKI 480 DLLGIPVIRPQVTETTALGAA LAG+ATGVYRD++ELS WRME TF P++ RD+AQ +I Sbjct: 421 DLLGIPVIRPQVTETTALGAAYLAGIATGVYRDLSELSSQWRMEHTFEPSIGRDQAQARI 480 Query: 481 QTWERAIKQVSAG 493 WE AI+QVSAG Sbjct: 481 HAWEAAIRQVSAG 493 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 140 bits (352), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 126/474 (26%), Positives = 201/474 (42%), Gaps = 32/474 (6%) Query: 3 YLLALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQVL 62 Y+L +D GT+S++S++F G +VA +E+ + +PG E DP + + L + + Sbjct: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAV 68 Query: 63 AQAGIKAADLAGLGITNQRETTVVWDRATGKPVHNAIVWQDRRTEAICAELRERGLTDAI 122 A ++A L + + + D A + + N+I W D R ++ +AI Sbjct: 69 KAAKAAPQEIALLSFSAAMHSVIALD-AENRLLSNSITWGDIRASVWAERIKHEHDGNAI 127 Query: 123 QAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTGGRLHVTDVS 182 +TG + P KL W+ P + RA R F + +L++ L G V D S Sbjct: 128 YRRTGTPVHPMSPLCKLMWMRHEKPDVFHRAAR----FVGIKEYLLYQLFGQW--VVDHS 181 Query: 183 NASRTMLWNIHTGNWDDELLEWLDVPRSMLPEVHPSAH---LYGHTDAGHLGAAVVIGGI 239 AS T ++N+ WD L L V LP P+ H A LG +V I Sbjct: 182 IASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFI 241 Query: 240 AGDQQSAL--FGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGERKQY 297 G L G + G T GT M + + L C K + Sbjct: 242 IGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRLF----CYALTEKHW 297 Query: 298 ALEGSVFIGGAVVQWLRD-------------GLKAIKNSTDVEELAASAPDSGGVVFVPS 344 + G V GG + +W+RD G+ + T + E P + G++F P Sbjct: 298 VVGGPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAE--KVRPGAEGLLFHPF 355 Query: 345 FTGLGAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLTAMVRDAVSPITELR 404 G AP WN +G+ GL+ H+ RAALE + F ++L A+ + V P + Sbjct: 356 MAGERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPAL-EELVGPTKRMM 414 Query: 405 VDGGASANNLLLQFQADLLGIPVIRPQVTETTALGAASLAGLATGVYRDMAELS 458 GG + + L Q AD+ V+ P+ E++ LGAA L A G+ ++ +S Sbjct: 415 ATGGFARSALWRQMMADIFNREVVVPESVESSCLGAAVLGAWALGLVPSLSVIS 468 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 126 bits (316), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 125/460 (27%), Positives = 202/460 (43%), Gaps = 33/460 (7%) Query: 1 MAYLLALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQ 60 M+YL +D GTS + ++ DE ++A+ R P P W E P WN+ L Sbjct: 1 MSYL-GIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAA 59 Query: 61 VLAQAGIKAADLAGLGITNQRETTVVWDRATGKPVHNAIVWQDRRTEAICAELRERGLTD 120 + QA L G+G++ Q + DR G + AI+W D R A C EL E + D Sbjct: 60 LRTQAPQAFQALRGIGLSGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVEL-EALVPD 117 Query: 121 AIQAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTGGRLHVTD 180 A+ A TG P F+ KL W+ P + + L +L W LTG V++ Sbjct: 118 AV-AITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLP----KDFLGWKLTGE--FVSE 170 Query: 181 VSNASRTMLWNIHTGNWDDELLEWLDVPRSMLPEVHPSAHLYGHTDAGHL---------- 230 +S+A+ T+ ++ +W + +L + RS +P + + G AG L Sbjct: 171 MSDAAGTLWLDVARRDWSERMLGATGLDRSHMPRL-----VEGSAVAGQLRDELRREWGI 225 Query: 231 GAAVVIGGIAGDQQSALFGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQ 290 VV+ G AGD ++ G + G A + G+ + T ++ G+ + C Sbjct: 226 SGPVVVAGGAGDNAASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCL 285 Query: 291 VGERKQYALEGSVFIGGAVVQWLRDGLKA--IKNSTDVEELAASAPDSGGVVFVPSFTGL 348 G+ Q ++ + A + WL G+ A + E ++ + +F+P G Sbjct: 286 PGQWHQMSV---ILSAAASLHWL-SGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGE 341 Query: 349 GAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLTAMVRDAVSPITELRVDGG 408 P+ + +AKG + G++ AH+A A +E +AF + A A ++ A + + GG Sbjct: 342 RTPHNDAAAKGVLFGMTPAHESAHLAYAVMEGVAF-AMADGYAALQAAGTTLESAAFVGG 400 Query: 409 ASANNLLLQFQADLLGIPVIRPQVTET-TALGAASLAGLA 447 S + A LGIP+ R E ALGAA L LA Sbjct: 401 GSRSAFWGVLCATALGIPLRRHAGAEVGAALGAARLGRLA 440 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 136/497 (27%), Positives = 222/497 (44%), Gaps = 35/497 (7%) Query: 5 LALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQVLAQ 64 L +D GTS ++++ D G++VA A P P W E P D W + T ++ Q Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 65 AGI-KAADLAGLGITNQRETTVVWDRATGKPVHNAIVWQDRRTEAICAELRERGLTDAIQ 123 G + + +G++ Q V+ D + + AI+W D R+ CAEL R + Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDE-RDEVLRPAILWSDSRSAPECAELESR--APRLH 119 Query: 124 AKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTV---DAWLIWNLTGGRLHVTD 180 G + P F+ KL W+ P L R TV WL +TG + V+D Sbjct: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRLKMTGRK--VSD 170 Query: 181 VSNASRTMLWNIHTGNWDDELLEWLDVPRSMLPEVHPSAHLYGH-----TDAGHLGAAVV 235 S+A+ T+ ++ NW DELL + R +P + + + G A L + V+ Sbjct: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVI 230 Query: 236 IGGIAGDQQSALFGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGERK 295 + G AGD ++ G +PG + GT + + D+ + G A C ++ Sbjct: 231 VAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 Query: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKNS--TDVEELAASAPDSGGVVFVPSFTGLGAPY 352 + + SV + A ++W + S ++E+L A ++ +F+P +G P+ Sbjct: 289 WHQM--SVMLSAASCLRWFCRLCSVDEKSLLAEIEQLDEQACNN-APLFLPYLSGERTPH 345 Query: 353 WNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLTAMVRDAVSPITELRVDGGASAN 412 +P A G GL+ A + A LE +AF L A+ R A + + EL + GG + + Sbjct: 346 NDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDAL-RAAGTDVAELSLVGGGARS 404 Query: 413 NLLLQFQADLLGIPVIRPQVTET-TALGAASLAGLATGVYRDMAELSEHWRMERTFLPTM 471 Q AD L + ++ +E ALGAA LA LA G D AE+ + + P Sbjct: 405 AYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLADG--GDEAEVCRKPAQQALYQPDP 462 Query: 472 SRDEA-QTKIQTWERAI 487 +R A Q ++Q + RAI Sbjct: 463 ARHAALQARLQRY-RAI 478 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 68.2 bits (165), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 56/205 (27%), Positives = 90/205 (43%), Gaps = 21/205 (10%) Query: 3 YLLALDQGTSSSRSIIFDETGAIVATAQQE---FRQIFPQPGWVEHDPMDLWNSQLQTCR 59 YL+ +D GT S+R+ +FD G ++A+A+ + FR + VE +W + Q R Sbjct: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRD--ERRARVEQSSAQIWEAVCQAVR 61 Query: 60 QVLAQAGIKAADLAGLGITNQRETTVVWDRA----TGKPVHNAIVWQDRRTEAICAELRE 115 + ++GI A +AG+G+ V A G P + IVW D R A+ R Sbjct: 62 AAVTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHR--ALEQAQRI 119 Query: 116 RGLTDAIQAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTGGR 175 + A+ + G V+ P KL W+ +++P + A F + +L W TG Sbjct: 120 NRMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAH----FFDLTDFLTWKATGS- 174 Query: 176 LHVTDVSNASRTMLWNI--HTGNWD 198 S+ + T W H WD Sbjct: 175 ---LQRSSCTVTCKWTYLAHEAGWD 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,555,300 Number of extensions: 64483 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 6 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)