BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0153.p (599 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herba... 1184 0.0 gi|300312097|ref|YP_003776189.1| lysyl-tRNA synthetase [Herbaspi... 68 2e-12 >gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 599 Score = 1184 bits (3062), Expect = 0.0, Method: Compositional matrix adjust. Identities = 567/599 (94%), Positives = 585/599 (97%) Query: 1 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDRVDVFKNA 60 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDR DVFKNA Sbjct: 1 MRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDREGLVQVVCDPDRADVFKNA 60 Query: 61 EAVRNEFCLRITGIVRNRPDGTSNANLTSGKIEVLAHELEVLNPSVTPPFQLDDDNLSET 120 E+VRNEFCLRITG+VRNRP+GT+NANL SGKIEVL HELEVLNPSVTPPFQLDDD+LSET Sbjct: 61 ESVRNEFCLRITGLVRNRPEGTTNANLKSGKIEVLCHELEVLNPSVTPPFQLDDDSLSET 120 Query: 121 TRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLVP 180 TRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLVP Sbjct: 121 TRLTHRVLDLRRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTPEGARDYLVP 180 Query: 181 SRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFM 240 SRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSF+ Sbjct: 181 SRVNAGEFFALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL 240 Query: 241 NEQEIRDLFEGMIRLVFKNVLDIDLPDPFPVMDFATAMGMYGSDKPDMRVKLEFTELTDV 300 NEQEIRDLFEGMIRLVFKN LDIDLP+PFPVMDFATAMG YGSDKPDMRVKLEFTELTDV Sbjct: 241 NEQEIRDLFEGMIRLVFKNALDIDLPNPFPVMDFATAMGKYGSDKPDMRVKLEFTELTDV 300 Query: 301 MKDVEFKVFAGAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEKA 360 MKDVEFKVF+GAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEKA Sbjct: 301 MKDVEFKVFSGAANSEGGRVVGLRVPGGAEMPRSEIDAYTQFVAIYGAKGLAYIKVNEKA 360 Query: 361 KGRDGLQSPIVKNLHDAALAAIVEKTGAQDGDLIFFGADKTKVVNDAIGALRVKIGHSDF 420 KGRDGLQSPIVKN+HDAAL AI+EKTGAQDGDLIFFGADK KVVNDAIGALRVKIGHS+F Sbjct: 361 KGRDGLQSPIVKNIHDAALNAILEKTGAQDGDLIFFGADKAKVVNDAIGALRVKIGHSEF 420 Query: 421 GKKNGLLDDAWRPLWVVDFPMFEYDEDNQRWVALHHPFTSPKDGHEDFISTNPAAAIAKA 480 GKKNGL DDAWRPLWVVDFPMFEYDED QRWVALHHPFTSPKDGHEDFISTNP AAIAKA Sbjct: 421 GKKNGLFDDAWRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHEDFISTNPGAAIAKA 480 Query: 481 YDLVLNGWEMGGGSVRIHRAEVQSKVFRALKINDEEARLKFGFLLDALQYGAPPHGGIAF 540 YD+VLNGWE+GGGSVRIHRA+VQSKVFRALKI+DEEA+LKFGFLLDALQYGAPPHGGIAF Sbjct: 481 YDMVLNGWELGGGSVRIHRADVQSKVFRALKIDDEEAKLKFGFLLDALQYGAPPHGGIAF 540 Query: 541 GLDRLVTMMAGAESIRDVIAFPKTQRAQDLLTQAPSAVDEKQLRELHIRLRNAEPAIKA 599 GLDRLVTMMAGAESIRDVIAFPKTQRAQ LLT APS VDEKQLRELHIRLRN EPA+K+ Sbjct: 541 GLDRLVTMMAGAESIRDVIAFPKTQRAQCLLTHAPSEVDEKQLRELHIRLRNTEPAVKS 599 >gi|300312097|ref|YP_003776189.1| lysyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 513 Score = 68.2 bits (165), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 65/264 (24%), Positives = 121/264 (45%), Gaps = 19/264 (7%) Query: 1 MRTQYCGLTTEALLGQ--TVSLCGWVHRRRDHGGVIFIDLRDREGL-----VQVVCDPDR 53 + +Y G EAL + TV++ G + +R G F L+D G+ +Q+ D Sbjct: 49 LHAKYDGFENEALEAEPVTVTVAGRMMLKRVMGKAAFATLQDASGVRADGRIQLYITRDV 108 Query: 54 VDVFKNAEAVRNEFCL-RITGIVRNRPDGTSNANLTSGKIEVLAHELEVLNPSVTPPFQL 112 + A A + L I G +GT T G++ V +L+++ S+ P Sbjct: 109 TG--EEAMAAFKHYDLGDILG-----AEGTLFKTKT-GELSVKVTKLKLITKSLRPLPDK 160 Query: 113 DDDNLSETTRLTHRVLDL-RRPQMQNNLRLRYKVAMEVRKFLDAQGFIDIETPMLTKSTP 171 + + R +DL + + + R +R+F++ GF+++ETPML P Sbjct: 161 FHGLADQEMKYRQRYVDLIMSEETRRTFKARTAAMSSIRRFMETHGFMEVETPML-HPIP 219 Query: 172 EGARDYLVPSRVNAGEF-FALPQSPQLFKQLLMVANFDRYYQITKCFRDEDLRADRQPEF 230 GA + NA + + +P+L+ + L+V F+R ++I + FR+E + PEF Sbjct: 220 GGAAAKPFITHHNALDMQMFMRIAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEF 279 Query: 231 TQIDCETSFMNEQEIRDLFEGMIR 254 T ++ ++++ Q + E +IR Sbjct: 280 TMMEFYAAYVDYQWLMGFTEQVIR 303 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 47/172 (27%), Positives = 76/172 (44%), Gaps = 31/172 (18%) Query: 399 DKTKVVNDAIGALRVKIGHSDFGKKNGLLDDAWRPLWVVDFPMFEYDEDNQRWVALHHPF 458 D+ + IGAL++ + + W P +++D+P+ Sbjct: 367 DQAPLAGAGIGALQLALFEETAESQ------LWNPTYIIDYPVE---------------- 404 Query: 459 TSPKDGHEDFISTNPAAAIAKAYDLVLNGWEMGGGSVRIHRAEVQSKVFRAL----KIND 514 SP D + A I + ++L + G E+ G ++ AE Q+ F A D Sbjct: 405 VSPLARASDTV-----AGITERFELFITGREIANGFSELNDAEDQAARFHAQVAAKDAGD 459 Query: 515 EEARLKFGFLLDALQYGAPPHGGIAFGLDRLVTMMAGAESIRDVIAFPKTQR 566 EEA + AL+YG PP GG G+DRL+ ++ + +IRDVI FP +R Sbjct: 460 EEAMYYDADYIRALEYGMPPAGGCGIGIDRLMMLITDSPNIRDVILFPHLRR 511 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,844,655 Number of extensions: 77584 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 3 Length of query: 599 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 508 Effective length of database: 1,188,985 Effective search space: 604004380 Effective search space used: 604004380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)