BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0145.p (694 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 752 0.0 gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 131 2e-31 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 87 4e-18 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 75 1e-14 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 67 4e-12 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 752 bits (1941), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/661 (61%), Positives = 511/661 (77%), Gaps = 4/661 (0%) Query: 30 DTGESSSLEHYVATQANFASIISAAMSLAMIIALFGVVVLISCYADDGYRKILADLISVQ 89 D S+S+E++ A+ A+++S+ + AM LAM + L G+VVL++ + YR++L+ L+ ++ Sbjct: 14 DIQNSASVEYFAASHAHYSSMATLAMGLAMAVILLGLVVLLASLLGEEYRRLLSGLLEMR 73 Query: 90 GSSAAGFILSGAALYLQCLNVGKISRKLALASRVLIVLLLLLSAGFLLQNLFGVKLLHGA 149 +A G IL +ALYL+C++ G +R+ L RV L +L A L G+ G Sbjct: 74 EMTAVGLILGASALYLRCIHGG--ARRSGL-RRVAAGLSWMLLALGLGTLAHGLMYALGW 130 Query: 150 FSVLSTAGSAFGAEIHPLAAGCFLLTALILLLQDLRIRGGHYPAEYLAFVLLGLSAIPLV 209 + I PL + F++ A +LLLQD R+ GG+YPAEYL+F L+GL+AIPLV Sbjct: 131 LPI-DQNHPLSPLVIPPLLSLGFVVMAAVLLLQDWRMLGGYYPAEYLSFALIGLAAIPLV 189 Query: 210 GYLYGINKLTFLRYSTGIPLLTALGFLLLGAALLMARPSHRLMSIIIGHAPGGNMLRRLL 269 GYLYG+++ +L + + LL+AL LL ALLMARP+H LM+II APGG MLR LL Sbjct: 190 GYLYGVSQFVYLDFPLPVSLLSALVMALLATALLMARPAHPLMAIITRIAPGGQMLRHLL 249 Query: 270 PSMLFLILLLNWLVSRGAERGLYSDQLMLPFLTLIDGALVVFVFWRFAGKLDREYGARMQ 329 P +FL+L + L++ G G +L+LP LTLI+G +V+F+FW A +LD EYG R + Sbjct: 250 PQTVFLLLAFSLLLNWGMTHGWILPELLLPTLTLINGVIVLFIFWGSASRLDSEYGERTR 309 Query: 330 SAAKLAEANSLLTAVSDSTREPIFVKNRQGLLVFANPALLQQVGKTWEEAMYRSSRELFT 389 +A KLAE ++LL AVS+ST +PIFVKNRQGL++FANPA L ++GKTWEE MYRSSRELF Sbjct: 310 NAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETMYRSSRELFL 369 Query: 390 EREEADRIDEDDKRVMAHGLPETIEQTLHLQHGIFTYQSTRVPWFDKAGNVLGVIGISTD 449 EEAD ID DD+RVMA G PE +EQTLHL G+ T+Q+ +VPWF K G+V GVIGISTD Sbjct: 370 VPEEADTIDRDDRRVMASGKPEKLEQTLHLPEGVVTFQTAKVPWFGKDGSVQGVIGISTD 429 Query: 450 ISERKRAEDSLREREAQLEKAVVERTALLRELTNHLETVREEEKRAIARELHDNMGASLT 509 I+ERK+AED LR+RE+QLEK V++RTALLRELTNHLETVREEEKRAIARELHDNMGASLT Sbjct: 430 ITERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIARELHDNMGASLT 489 Query: 510 ALSMHLESVYKILPEDEKWQERKPRMQGLMSSLVSTTRRIQTELRPNMLDLFGLKAAITE 569 ALSMHLE VY+ILP DEKW +RK RMQGLM SLV+TTRRIQTELRPN LDLFGLKAAI+E Sbjct: 490 ALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDLFGLKAAISE 549 Query: 570 QLDELAERTGITCKSSLPDEDVTIGHEMEIAIYRMLQEMLNNVTKHAKATQVDVILDVDE 629 QLDEL ERTGI C +SLPDEDV +GHEMEIAIYRMLQEMLNNVTKHAKA++VDVILD+DE Sbjct: 550 QLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASKVDVILDIDE 609 Query: 630 EQVSLTVRDNGVGIPQERQDNNKTYGLRGLRERATFFGGNVVIRSTPGNGSIISIRLPLA 689 + V+LTVRD+GVGIP+ER+DN+KTYGLRGLRERATFFGG V IRSTPG G++I+I LP+ Sbjct: 610 DHVALTVRDDGVGIPEERRDNHKTYGLRGLRERATFFGGEVDIRSTPGKGALITISLPIR 669 Query: 690 P 690 P Sbjct: 670 P 670 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 131 bits (329), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 111/378 (29%), Positives = 192/378 (50%), Gaps = 43/378 (11%) Query: 330 SAAKLAEANSLLTAVSDSTREPIFVKNRQGLLVFANPALLQQVGKTWEEAMYRSSRELFT 389 SA L E + A ++ P ++K+ Q + NPA G T + + EL + Sbjct: 121 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPGDMDGLTDLELMS 180 Query: 390 EREEADRIDEDDKRVMAHGLPETIEQTLHLQHGIFTYQST----RVPWFDKAGNVLG--V 443 A E+D++V+ LH Q +F + V F +G G V Sbjct: 181 SGAAAAS-QENDRKVLEQKC------ELHTQETVFDHNHNARILDVVRFPISGEGKGDMV 233 Query: 444 IGISTDISERKRAEDSLREREAQLEKAVVERTALLRELTNHLETVREEEKRAIARELHDN 503 G++ D++ER+++ L + ++R LL ++ + V+E E++ +AR LHD+ Sbjct: 234 AGLAIDVTEREQSRRDL--------ELAIQRQQLL---SSRVIEVQERERQHLARGLHDD 282 Query: 504 MGASLTALSMHLESVYKILPEDEKWQERKPRMQG---LMSSLVSTTRRIQTELRPNMLDL 560 +G SLTAL ++LE++ K+ + P +Q +++++++ R + +LRP LD Sbjct: 283 VGQSLTALKINLETIKKMGSLE------GPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDN 336 Query: 561 FGLKAAI---TEQLDELAERTGITCKSSLPDEDVTIGHEMEIAIYRMLQEMLNNVTKHAK 617 GL AA+ EQ +LA G +SS DE + H++E +R++QE + N+ +HA+ Sbjct: 337 LGLIAALRSYVEQKSKLANVNGW-FESSGHDE--QLHHDIENTAFRIVQEAVTNILRHAR 393 Query: 618 ATQVDVILDVDEEQVSLTVRDNGVGIPQERQDNN----KTYGLRGLRERATFFGGNVVIR 673 + V +D +QV L++RD+G G ER +N ++GL + ERA GG++ I Sbjct: 394 CQNIWVKIDRQHDQVYLSIRDDGRGFDIERARSNAISGTSFGLLNMEERAVLVGGSMEIT 453 Query: 674 STPGNGSIISIRLPLAPV 691 S PG G+ I + LP+AP+ Sbjct: 454 SAPGAGTEIVMHLPVAPI 471 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 87.0 bits (214), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 63/224 (28%), Positives = 113/224 (50%), Gaps = 19/224 (8%) Query: 476 ALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLESVYKIL---PEDEK----- 527 A LR+L + +EEE+ ++R+LHD + L ++ + +E+ L PE Sbjct: 233 AKLRQLAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAG 292 Query: 528 WQERKPRMQGLMSSLVSTTRRIQTELRPNMLDLFGLKAAITEQLDELAERTGITCK---- 583 +++ ++ G+++ + RRI +LRP +LD GL AA+ E + G + Sbjct: 293 FEKTATQINGVLAEV----RRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFE 348 Query: 584 -SSLPDEDVTIGHEMEIAIYRMLQEMLNNVTKHAKATQVDVILDVDEEQVSLTVRDNGVG 642 + D+ T+ + ++R+ QE L N+ +HA A+++ V L + L V+DNG G Sbjct: 349 NQAQADDGATLPDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHG 408 Query: 643 IPQERQDNNKTY--GLRGLRERATFFGGNVVIRSTPGNGSIISI 684 + D++ + GLR +RER GG + I S+P SI ++ Sbjct: 409 FDLGQVDDHSRHGIGLRNMRERIEAVGGELGIDSSPAGTSISAV 452 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 54/199 (27%), Positives = 105/199 (52%), Gaps = 6/199 (3%) Query: 492 EKRAIARELHDNMGASLTALSMHLESVYKILPED--EKWQERKPRMQGLMSSLVSTTRRI 549 E+ +A+ LHD++ L L++ ++ + L D ++ ++ P ++ + R + Sbjct: 446 ERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLDEIRDIAPLLRTGVQENYEDVREL 505 Query: 550 QTELRPNMLDLFGLKAAITEQLDELAERTGITCKSSLPDEDVTIGHEMEIAIYRMLQEML 609 R + D L + + + LD+L ++G+ + +L + + E ++ + +LQE L Sbjct: 506 LLNFRTRLQD-SNLDSEMRKALDKLQRQSGLQGQITLTGDGPPLAPEQQLQVLFILQEAL 564 Query: 610 NNVTKHAKATQVDVILDVDEEQVSLTVRDNGVGIPQE--RQDNNKTYGLRGLRERATFFG 667 +N+ KHA+A QV + +D + + +TV D+G G E R + GLR ++ERA G Sbjct: 565 SNIRKHARAQQVQISVD-NRQDFRMTVSDDGHGFTLESARAKGLEHVGLRIMQERAERLG 623 Query: 668 GNVVIRSTPGNGSIISIRL 686 N+ I S PG+G+ I++ L Sbjct: 624 ANLEIDSQPGHGTRIALTL 642 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 67.4 bits (163), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 56/212 (26%), Positives = 105/212 (49%), Gaps = 16/212 (7%) Query: 488 VREEEKRAIARELHDNMGASLTALSMHLESVYKIL-------PEDEKWQERKPRMQGLMS 540 +RE+E+ IAR+LHD++G L + M L + + L P E + R+ L+ Sbjct: 150 IREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQ-----LLQ 204 Query: 541 SLVSTTRRIQTELRPNMLDLFGLKAAITEQLDELAERTGITCKSSLPDEDVTIGHEMEIA 600 +++ I EL P +++ FGL A+ Q ++ + C+ P + + Sbjct: 205 QAMASMHSIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPTLEQEDRFLILG 263 Query: 601 IYRMLQEMLNNVTKHAKATQVDVILDVDEEQVSLTVRDNGVGI-PQERQD--NNKTYGLR 657 + R+L+E +N +H +A+ + V L + + ++ + + D+G GI P ++ +GLR Sbjct: 264 LLRILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVAPRHGHGLR 323 Query: 658 GLRERATFFGGNVVIRSTPGNGSIISIRLPLA 689 +RERA GG + I + G +I +P+A Sbjct: 324 NMRERAQALGGELFIEAGQDGGVVIRTLIPVA 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,767,091 Number of extensions: 62052 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 292 Number of HSP's successfully gapped: 6 Length of query: 694 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 602 Effective length of database: 1,184,250 Effective search space: 712918500 Effective search space used: 712918500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)