BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0133.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Her... 500 e-143 gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate A... 204 6e-54 gi|300312621|ref|YP_003776713.1| ABC transporter ATPase [Herbasp... 184 6e-48 gi|300310818|ref|YP_003774910.1| nitrate/sulfonate/bicarbonate A... 181 5e-47 gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase ... 179 2e-46 >gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 272 Score = 500 bits (1287), Expect = e-143, Method: Compositional matrix adjust. Identities = 241/272 (88%), Positives = 259/272 (95%), Gaps = 3/272 (1%) Query: 1 MDMHN---KFIDINDVEMVFHTKKGSFHALRDINLTVQKGEFITLIGHSGCGKSTLLNLI 57 M+M N KFIDI+ VEM+FHTKKG FHALR+INLTV KGEF+TLIGHSGCGKSTLLNLI Sbjct: 1 MNMDNINGKFIDIHGVEMLFHTKKGVFHALREINLTVAKGEFVTLIGHSGCGKSTLLNLI 60 Query: 58 AGLLKPSDGLLLCANREIAGPSPERSVVFQNHSLLPWLTCYENIFLGVERVFGATENRTQ 117 AGLLKPS+G+LLCANREI GP+PER+VVFQNHSLLPWL+CYENI+LGVERVFGATENRTQ Sbjct: 61 AGLLKPSEGVLLCANREIGGPAPERAVVFQNHSLLPWLSCYENIYLGVERVFGATENRTQ 120 Query: 118 LRDRTRAALALVGLTAAESKRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTR 177 LRDRTRAALALVGL+AAE+KRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTR Sbjct: 121 LRDRTRAALALVGLSAAENKRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTR 180 Query: 178 AHLQDELLKIVAKTESTVVMVTHDVDEAVLLSDRIVMMTNGPAATIGEILEVKLARPRER 237 AHLQDELLKIVAKT+STVVMVTHDVDEAVLLSDRIVMMTNGPAATIGE+LEVKLARPR+R Sbjct: 181 AHLQDELLKIVAKTDSTVVMVTHDVDEAVLLSDRIVMMTNGPAATIGEVLEVKLARPRDR 240 Query: 238 VALAQDARYSEYRTTVLEFLYRKQAHPAKEAA 269 V LAQD Y +YRT VLEFLYRKQAHPA+EAA Sbjct: 241 VVLAQDPEYGQYRTAVLEFLYRKQAHPAREAA 272 >gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 204 bits (519), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 113/262 (43%), Positives = 159/262 (60%), Gaps = 4/262 (1%) Query: 8 IDINDVEMVFHTKKGSFHALRDINLTVQKGEFITLIGHSGCGKSTLLNLIAGLLKPSDGL 67 I I V VF T AL+DINL + G+F+ L+G SGCGKSTLLN IAG PS G Sbjct: 10 IRIQGVHKVFKTDDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAIAGFALPSSGQ 69 Query: 68 LLCANREIAGPSPERSVVFQNHSLLPWLTCYENIFLGVERVFGATENRTQLRDRTRAALA 127 +L ++ ++ P P+R +VFQ ++L PW+T +N+ G+E + G + ++ R L Sbjct: 70 ILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLE-IKGTP--KAEIHQRVTQLLD 126 Query: 128 LVGLTAAESKRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTRAHLQDELLKI 187 +GL ++ P ++SGGM+QRV IAR LA++ ++LMDEPFGALDALTR +LQDELL+I Sbjct: 127 KLGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRRNLQDELLRI 186 Query: 188 VAKTESTVVMVTHDVDEAVLLSDRIVMMTNGPAATIGEILEVKLARPRERVALAQDARYS 247 + T+V VTH ++EA+ L+DRIV+MT P ++L V L R R+ +A Sbjct: 187 WDEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDML-VNLPRLRDPADPEFNALKR 245 Query: 248 EYRTTVLEFLYRKQAHPAKEAA 269 E V+E R K AA Sbjct: 246 ELGQLVMEEQQRHHNAEMKAAA 267 >gi|300312621|ref|YP_003776713.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 184 bits (468), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 95/219 (43%), Positives = 136/219 (62%), Gaps = 7/219 (3%) Query: 8 IDINDVEMVFHTKKGSFHALRDINLTVQKGEFITLIGHSGCGKSTLLNLIAGLLKPSDGL 67 + N+V F G H + + + GE + L+G SGCGKSTLL++ AGL KPS G Sbjct: 3 LSFNNVAKHF----GDLHVIEEFTREIASGELVALVGPSGCGKSTLLHMAAGLEKPSSGS 58 Query: 68 LLCANREIAGPSPERSVVFQNHSLLPWLTCYENIFLGVERVFGATENRTQLRDRTRAALA 127 + + I GP PER+++FQ ++L PWLT +N+ L +E F + + + + ++ R L Sbjct: 59 VAGDGQTIRGPHPERTLMFQENALYPWLTLQQNVALALE--FQSVDKK-KAAEQARVWLE 115 Query: 128 LVGLTAAESKRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTRAHLQDELLKI 187 V L E P+++SGGM+QR +ARA +PK LL+DEPFGALDALTR LQD L + Sbjct: 116 NVKLKGFEHYYPHQVSGGMRQRAALARAFISQPKALLLDEPFGALDALTRMTLQDALRDL 175 Query: 188 VAKTESTVVMVTHDVDEAVLLSDRIVMMTNGPAATIGEI 226 + + TVV+VTHDVDEA+ L+DRI + + PA + E Sbjct: 176 IRQQGPTVVLVTHDVDEALFLADRIFVFSPRPAKVLKEF 214 >gi|300310818|ref|YP_003774910.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 265 Score = 181 bits (459), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 108/233 (46%), Positives = 140/233 (60%), Gaps = 5/233 (2%) Query: 22 GSFHALRDINLTVQKGEFITLIGHSGCGKSTLLNLIAGLLKPSDGLLLCANREIAGPSPE 81 GS + I+L ++ GEF+ L+G SGCGKSTLLN IAG S G + + + GP + Sbjct: 17 GSNDIMAGIDLDIRAGEFVCLLGPSGCGKSTLLNAIAGFQDVS-GEISVDGKRVKGPGLD 75 Query: 82 RSVVFQN-HSLLPWLTCYENIFLGVERVFGATENRTQLRDRTRAALALVGLTAAESKRPN 140 R VVFQ+ +L PWL+ EN+ G+ ++ G + ++LVGL A K P Sbjct: 76 RGVVFQSSEALFPWLSVRENVEYGL-KLRGVASAERRAA--AERYVSLVGLKHAIDKFPA 132 Query: 141 EISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTRAHLQDELLKIVAKTESTVVMVTH 200 E+SGGM+QR IAR L EP V+LMDEPFGALDA TR LQ EL +I T T+V VTH Sbjct: 133 ELSGGMRQRAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIWKSTGCTIVFVTH 192 Query: 201 DVDEAVLLSDRIVMMTNGPAATIGEILEVKLARPRERVALAQDARYSEYRTTV 253 D+ EA+LL+DR+V MT GP A I I EV L PR+ A A Y+ R + Sbjct: 193 DIWEAILLADRVVTMTAGPRAHIKTIEEVHLPHPRDPADPACMALYARIRDDI 245 >gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 179 bits (455), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 90/217 (41%), Positives = 136/217 (62%), Gaps = 4/217 (1%) Query: 8 IDINDVEMVFHTK-KGSFHALRDINLTVQKGEFITLIGHSGCGKSTLLNLIAGLLKPSDG 66 + + + + F + K HAL ++L + G F+ +G SGCGKSTLLN +AG L S+G Sbjct: 2 LKVENASVYFSGRDKQRVHALDRVSLDIPNGGFVVALGTSGCGKSTLLNAMAGFLPLSEG 61 Query: 67 LLLCANREIAGPSPERSVVFQNHSLLPWLTCYENIFLGVERVFGATENRTQLRDRTRAAL 126 + + ++GP ER VVFQ SLLPW + +N+ LG++ A +++ R+R + L Sbjct: 62 RISLDGQPVSGPGAERGVVFQKDSLLPWKSVQDNVALGLKF---AGVSKSDSRERAQELL 118 Query: 127 ALVGLTAAESKRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTRAHLQDELLK 186 LVGL P E+SGGM+QRVG+ARALA PK+LLMDEPFGALD++TR +Q+ L+ Sbjct: 119 RLVGLQDFARAAPYELSGGMRQRVGLARALAPNPKILLMDEPFGALDSMTREQMQELLVS 178 Query: 187 IVAKTESTVVMVTHDVDEAVLLSDRIVMMTNGPAATI 223 + A+T V +TH ++EA+ L +++M+ P + Sbjct: 179 VWARTGKQVFFITHSIEEALFLGTEVIVMSPRPGRIV 215 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 768,799 Number of extensions: 29577 Number of successful extensions: 527 Number of sequences better than 1.0e-08: 131 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 144 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)